Endothelial dysfunction and arterial stiffness in pre-eclampsia demonstrated by the EndoPAT method

Meeme, Allen; Ga, Buga; Mammen, M.; Namugowa, Ambrose Vincent

doi:10.5830/cvja-2016-047

Cited by 27 publications

(22 citation statements)

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“…Multiple investigations of PE reveal that poor placentation is linked to endothelial dysfunction, endoplasmic reticulum stress, apoptosis, and most importantly, OS of the placenta. [23][24][25] Inhibition of the p38MAPK signaling pathway could reduce OS both in vivo and in vitro in PE was verified. 17 The purpose of the present study is to assess the roles of NCAM1d in OS in pregnancy complicated by PE with the participation of the p38MAPK signaling pathway.…”

Section: Discussionmentioning

confidence: 87%

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siRNA‐mediated NCAM1 gene silencing suppresses oxidative stress in pre‐eclampsia by inhibiting the p38MAPK signaling pathway

Zhang

Han

2019

J of Cellular Biochemistry

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Pre‐eclampsia (PE), whose pathophysiology and etiology remain undefined, represents a leading consequence of fetal and maternal mortality and morbidity. Oxidative stress (OS) is recognized to involve in this disorder. In this study, we hypothesized that neural cell adhesion molecule 1 (NCAM1) gene silencing would suppress the OS in the pregnancy complicated by PE. Initially, clinical samples were collected for determination of NCAM1 expression in placental tissues and levels of OS products in blood. To assess the regulatory mechanism of NCAM1 knockdown on OS, we used small interfering RNA (siRNA) to silence NCAM1 expression in human umbilical vein endothelial cells (HUVECs). Next, cells were treated with or without hypoxia/reoxygenation to observe the level changes of OS products and p38 mitogen‐activated protein kinase (p38MAPK) pathway‐related genes. Finally, an evaluation of HUVEC migration and invasion abilities was conducted by wound‐healing and transwell assays. Placenta of pregnancy with PE presented significantly increased NCAM1 expression in comparison to placenta of normal pregnancy. Meanwhile, enhanced OS in blood of pregnant women with PE was observed relative to women with normal pregnancy. siRNA‐mediated knockdown of NCAM1 gene could inhibit the p38MAPK signaling pathway, repress OS, and promote cell migration and invasion in HUVECs, indicating that NCAM1 inhibition could reduce the influence of PE. Importantly, blocking the p38MAPK signaling pathway reversed the inhibitory role of NCAM1 gene silencing on PE. Collectively, this study defines potential role of NCAM1 gene silencing as a therapeutic target in PE through inhibiting OS and enhancing HUVEC migration and invasion by disrupting the p38MAPK signaling pathway.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 89%

siRNA‐mediated NCAM1 gene silencing suppresses oxidative stress in pre‐eclampsia by inhibiting the p38MAPK signaling pathway

Zhang

Han

2019

J of Cellular Biochemistry

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“…The majority of studies on the assessment of vascular endothelial function using peripheral arterial tonometry and EndoPAT 2000 device was performed in women during gestation with the aim to describe potential association between vascular endothelial dysfunction and pregnancy-induced hypertension or preeclampsia [20,23,[50][51][52][53], sleep-disordered breathing in women with preeclamptic toxaemia [54], and smoking status [55].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Vascular Endothelial Function in Young and Middle-Aged Women with Respect to a History of Pregnancy, Pregnancy-Related Complications, Classical Cardiovascular Risk Factors, and Epigenetics

Hromadníková

Dvořáková

Krofta

2020

IJMS

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The aim of the study was to examine the effect of previous pregnancies and classical cardiovascular risk factors on vascular endothelial function in a group of 264 young and middle-aged women 3 to 11 years postpartum. We examined microvascular functions by peripheral arterial tonometry and EndoPAT 2000 device with respect to a history of gestational hypertension, preeclampsia, fetal growth restriction, the severity of the disease with regard to the degree of clinical signs and delivery date. Besides, we compared Reactive Hyperemia Index (RHI) values and the prevalence of vascular endothelial dysfunction among the groups of women with normal and abnormal values of BMI, waist circumference, systolic and diastolic blood pressures, heart rate, total serum cholesterol levels, serum high-density lipoprotein cholesterol levels, serum low-density lipoprotein cholesterol levels, serum triglycerides levels, serum lipoprotein A levels, serum C-reactive protein levels, serum uric acid levels, and plasma homocysteine levels. Furthermore, we determined the effect of total number of pregnancies and total parity per woman, infertility and blood pressure treatment, presence of trombophilic gene mutations, current smoking of cigarettes, and current hormonal contraceptive use on the vascular endothelial function. We also examined the association between the vascular endothelial function and postpartum whole peripheral blood expression of microRNAs involved in pathogenesis of cardiovascular/cerebrovascular diseases (miR-1-3p, miR-16-5p, miR-17-5p, miR-20a-5p, miR-20b-5p, miR-21-5p, miR-23a-3p, miR-24-3p, miR-26a-5p, miR-29a-3p, miR-92a-3p, miR-100-5p, miR-103a-3p, miR-125b-5p, miR-126-3p, miR-130b-3p, miR-133a-3p, miR-143-3p, miR-145-5p, miR-146a-5p, miR-155-5p, miR-181a-5p, miR-195-5p, miR-199a-5p, miR-210-3p, miR-221-3p, miR-342-3p, miR-499a-5p, and miR-574-3p). A proportion of overweight women (17.94% and 20.59%) and women with central obesity (18.64% and 21.19%) had significantly lower RHI values at 10.0% false positive rate (FPR) both before and after adjustment of the data for the age of patients. At 10.0% FPR, a proportion of women with vascular endothelial dysfunction (RHI ≤ 1.67) was identified to have up-regulated expression profile of miR-1-3p (11.76%), miR-23a-3p (17.65%), and miR-499a-5p (18.82%) in whole peripheral blood. RHI values also negatively correlated with expression of miR-1-3p, miR-23a-3p, and miR-499a-5p in whole peripheral blood. Otherwise, no significant impact of other studied factors on vascular endothelial function was found. We suppose that screening of these particular microRNAs associated with vascular endothelial dysfunction may help to stratify a highly risky group of young and middle-aged women that would benefit from early implementation of primary prevention strategies. Nevertheless, it is obvious, that vascular endothelial dysfunction is just one out of multiple cardiovascular risk factors which has only a partial impact on abnormal expression of cardiovascular and cerebrovascular disease associated microRNAs in whole peripheral blood of young and middle-aged women.

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“…They reported that women with preeclampsia show impaired endothelial function (significantly low RHI scores [OR: 1.70, 95% CI: 1.04-3.61]) compared with normotensive pregnant women (OR: 1.81, 95% CI: 1.18-4.62; p < 0.05). 8 Although these results seemed to confirm endothelial dysfunction in preeclampsia women, they only evaluated women at the time of diagnosis. They did not study the predictive ability of assessments of endothelial dysfunction.…”

Section: Discussionmentioning

confidence: 92%

“…Although this method has been used extensively in nonpregnant patients, few studies have examined its utility in predicting preeclampsia. 6,8,9 In one retrospective study, researchers measured postdelivery endothelial function of women who experienced preeclampsia to determine whether residual endothelial dysfunction may contribute to future cardiovascular issues, and found that there was persistent microcirculatory dysfunction in women with previous pregnancies complicated by preeclampsia. 4,5 Whether this technique can be used to predict preeclampsia in women with high-risk factors, by detecting early signs of endothelial dysfunction, is currently not known.…”

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confidence: 99%

Predicting Preeclampsia with Noninvasive Measures of Endothelial Dysfunction: A Pilot Study

Emeasoba

McLaren

Landau

et al. 2020

AJP Rep

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Objective This study evaluates the assessment of endothelial function and its prediction for preeclampsia among women with high-risk factors. Study Design A prospective cohort study of 107 pregnant women at 20 weeks or greater gestation with risk factors for developing preeclampsia. Endothelial dysfunction was assessed using peripheral arterial tonometry by generating a reactive hyperemia index (RHI) score. An index score of <1.67 was defined as endothelial dysfunction. The primary outcome was preeclampsia. Logistic regression was used to predict preeclampsia from RHI scores, body mass index, gestational age at RHI evaluation, history of preeclampsia, history of pregestational diabetes mellitus, chronic hypertension, and fetal number. A receiver operating characteristic plot was constructed to predict preeclampsia from the RHI score. Results Among 107 women, 99 had interpretable RHI scores. Among those with an abnormal RHI (n = 61), 17 (28%) developed preeclampsia. Among women with a normal score (n = 38), six (16%) developed preeclampsia (p = 0.166). After logistic regression, there was no significant association. A receiver operating characteristic plot also revealed no association between RHI score and preeclampsia. Conclusion An abnormal RHI score using peripheral arterial tonometry indicating endothelial dysfunction was not predictive of developing preeclampsia in this cohort. Future studies are needed to further evaluate this relationship.

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Endothelial dysfunction and arterial stiffness in pre-eclampsia demonstrated by the EndoPAT method

Cited by 27 publications

References 47 publications

siRNA‐mediated NCAM1 gene silencing suppresses oxidative stress in pre‐eclampsia by inhibiting the p38MAPK signaling pathway

siRNA‐mediated NCAM1 gene silencing suppresses oxidative stress in pre‐eclampsia by inhibiting the p38MAPK signaling pathway

Evaluation of Vascular Endothelial Function in Young and Middle-Aged Women with Respect to a History of Pregnancy, Pregnancy-Related Complications, Classical Cardiovascular Risk Factors, and Epigenetics

Predicting Preeclampsia with Noninvasive Measures of Endothelial Dysfunction: A Pilot Study

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