Endothelial nitric oxide synthase in the vascular wall: Mechanisms regulating its expression and enzymatic function

Demosthenous, Michael; Antoniades, Charalambos; Tousoulis, Dimitris; Margaritis, Marios; Marinou, Kyriakoula; Stefanadis, Christodoulos

doi:10.1016/j.artres.2011.03.003

Cited by 4 publications

(3 citation statements)

References 141 publications

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“…In the absence of its substrate (L-arginine), the eNOS generates O 2 − instead of NO. NO exerts a number of beneficial antiatherogenic effects and reacts with a variety of other targets, acting to modulate ion channel function, intracellular signal transduction and gene expression ( 7 , 16 ). Therefore, the loss of NO bioavailability is a key feature of endothelial dysfunction, and can occur due to multiple pathways altering NO synthesis or biodegradation.…”

Section: Discussionmentioning

confidence: 99%

“…Guanxin Shutong capsule (GXSTC) is a Chinese medicinal formula that is widely used clinically for the treatment of numerous symptoms, including palpitation, restlessness, dyspnea, chest pain, dizziness and fatigue. In addition, GXSTC is used to promote blood circulation, remove blood stasis and prevent CVDs ( 7 , 8 ). Our previous study suggested that oral GXSTC could protect the heart against oxidative stress and apoptosis in rats with myocardial infarction ( 9 ).…”

Section: Introductionmentioning

confidence: 99%

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Protective effects of Guanxin Shutong capsule drug-containing serum on tumor necrosis factor-α-induced endothelial dysfunction through nicotinamide adenine dinucleotide phosphate oxidase and the nitric oxide pathway

Cao

Liu

Huang

et al. 2014

Experimental and Therapeutic Medicine

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The Chinese medicinal formula Guanxin Shutong capsule (GXSTC) has been used for almost 10 years as a clinical treatment for chest pain, depression, palpitation and cardiovascular diseases. The aim of this study was to investigate the effects of GXSTC drug-containing serum on tumor necrosis factor-α (TNF-α)-stimulated endothelial cells. Cell viability was measured by MTT assay, and nitric oxide (NO) levels and NO synthase (NOS) activity were measured as standards of endothelial dysfunction. Malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity were evaluated using commercial kits. In addition, the protein expression of endothelial NOS (eNOS), AKT and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunits was examined to evaluate the effect of GXSTC drug-containing serum on ECV304 cells. GXSTC significantly reversed the decrease in NO production induced by TNF-α (5 ng/ml) in ECV304 cells. The expression of NADPH oxidase subunits was increased by TNF-α treatment, but markedly inhibited by treatment with GXSTC in TNF-α-stimulated cells. In summary, GXSTC increased the production of NO in ECV304 cells and exerted a protective effect on ECV304 cells stimulated with TNF-α by upregulating the mRNA and protein expression of eNOS. This was accompanied by increased SOD activity and reduced MDA levels. These results suggested that GXSTC protects the endothelium via the NO pathway and exhibits antioxidant effects.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protective effects of Guanxin Shutong capsule drug-containing serum on tumor necrosis factor-α-induced endothelial dysfunction through nicotinamide adenine dinucleotide phosphate oxidase and the nitric oxide pathway

Cao

Liu

Huang

et al. 2014

Experimental and Therapeutic Medicine

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“…The − 786T > C polymorphism of NOS3 gene in the promoter region is a missense mutation, which is located in exon 7 of the gene and replaces thymine with cytosine at position 786. The 896G > T polymorphism of NOS3 gene in the coding region, also localized in exon 7, replaces guanine with thymine at position 894, resulting in a change of the amino acid sequence Glu298Asp [ 11 ]. Human EDN-1 gene is located on chromosome 6p23-p24 and consists of 6836 nucleotides [ 12 ], [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Potential role of eNOS and EDN-1 gene polymorphisms in the development and progression of retinopathy of prematurity

Choręziak-Michalak

Gotz‐Więckowska

Chmielarz-Czarnocińska

et al. 2023

BMC Ophthalmol

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The aim of this study was to investigate the association between selected polymorphisms of nitric oxide synthetase (eNOS) and endothelin-1 (EDN-1) with the occurrence and progression of retinopathy of prematurity (ROP). A prospective study was conducted on 90 preterm infants (44 female), comparing 39 cases with ROP and 51 controls without ROP. Patients who developed ROP were further divided into two subgroups—those with spontaneous regression of the disease and those with ROP requiring treatment. We found that preterm infants with TT genotype eNOS 894G > T had a 12.8-fold higher risk of developing ROP requiring treatment (p = 0.02). Our results showed that allele T of eNOS894G > T polymorphism was significantly more prevalent in ROP patients requiring treatment (p = 0.029). We also investigated preterm infants with TC genotype eNOS − 786 T > C and found an 8.8-fold higher risk developing of ROP requiring treatment (p = 0.021). Our results didn’t show any association between EDN-1 5665G > T polymorphism and ROP development. The eNOS polymorphisms appears to influence incidence of ROP requiring treatment in preterm infants. Future research on single nucleotide polymorphisms may provide important information about the pathogenetic mechanisms underlying the development of ROP.

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Role of NO and NO synthases in oncogenesis

2015

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Endothelial nitric oxide synthase in the vascular wall: Mechanisms regulating its expression and enzymatic function

Cited by 4 publications

References 141 publications

Protective effects of Guanxin Shutong capsule drug-containing serum on tumor necrosis factor-α-induced endothelial dysfunction through nicotinamide adenine dinucleotide phosphate oxidase and the nitric oxide pathway

Protective effects of Guanxin Shutong capsule drug-containing serum on tumor necrosis factor-α-induced endothelial dysfunction through nicotinamide adenine dinucleotide phosphate oxidase and the nitric oxide pathway

Potential role of eNOS and EDN-1 gene polymorphisms in the development and progression of retinopathy of prematurity

Role of NO and NO synthases in oncogenesis

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