Endothelial nitric oxide synthase is a molecular vascular target for the Chinese herb Danshen in hypertension

Kim, David D.; Sánchez, Fabiola A.; Durán, Ricardo G.; Kanetaka, T.; Durán, Walter N.

doi:10.1152/ajpheart.01027.2006

Cited by 68 publications

(57 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is effective in removing blood stasis and promoting Qi. Herbs such as Chishao (red peony root) [137], Danshen ( Salvia miltiorrhiza ) [138], Yimucao ( Leonurus japonicus ), and Chuanxiong ( Ligusticum chuanxiong Hort) [139] could also lower BP. When aiming to remove qi stagnation and blood stasis, use herbs to promote qi circulation by taking herbs to remove blood stasis.…”

Section: Discussion and Perspectivesmentioning

confidence: 99%

Traditional Chinese Medicine Syndromes for Essential Hypertension: A Literature Analysis of 13,272 Patients

Wang

Xiong

Liu

2014

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Background. To simplify traditional Chinese medicine syndrome differentiation and allow researchers to master syndrome differentiation for hypertension, this paper retrospectively studied the literature and analyzed syndrome elements corresponding to hypertension syndromes. Methods. Six databases including PubMed, EMBASE, Chinese Bio-Medical Literature Database, Chinese National Knowledge Infrastructure, Chinese Scientific Journal Database, and Wan-fang Data were searched from 1/January/2003 to 30/October/2013. We included all clinical literature testing hypertension syndromes and retrospectively studied the hypertension literature published from 2003 to 2013. Descriptive statistics calculated frequencies and percentages. Results. 13,272 patients with essential hypertension were included. Clinical features of hypertension could be attributed to 11 kinds of syndrome factors. Among them, seven syndrome factors were excess, while four syndrome factors were deficient. Syndrome targets were mainly in the liver and related to the kidney and spleen. There were 33 combination syndromes. Frequency of single-factor syndromes was 31.77% and frequency of two-factor syndromes was 62.26%. Conclusions. Excess syndrome factors of hypertension patients include yang hyperactivity, blood stasis, phlegm turbidity, internal dampness, and internal fire. Deficient syndrome factors of hypertension patients are yin deficiency and yang deficiency. Yin deficiency with yang hyperactivity, phlegm-dampness retention, and deficiency of both yin and yang were the three most common syndromes in clinical combination.

show abstract

Section: Discussion and Perspectivesmentioning

confidence: 99%

Traditional Chinese Medicine Syndromes for Essential Hypertension: A Literature Analysis of 13,272 Patients

Wang

Xiong

Liu

2014

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…SD male rats (160 -230 g, Charles River) were anaesthetized with sodium pentobarbital (50 mg/kg ip). The right cremaster muscle was prepared for intravital microscopy (10,26). Pentobarbital sodium was continuously administered (0.03 mg/min, 0.1 ml/h iv) via catheter implanted in right jugular veins to maintain anesthesia.…”

Section: Methodsmentioning

confidence: 99%

Soluble guanylyl cyclase is a target of angiotensin II-induced nitrosative stress in a hypertensive rat model

Crassous

Couloubaly

Huang

et al. 2012

American Journal of Physiology-Heart and Circulatory Physiology

Self Cite

View full text Add to dashboard Cite

Soluble guanylyl cyclase is a target of angiotensin II-induced nitrosative stress in a hypertensive rat model. Am J Physiol Heart Circ Physiol 303: H597-H604, 2012. First published June 22, 2012; doi:10.1152/ajpheart.00138.2012 by activating soluble guanylyl cyclase (sGC) is involved in vascular homeostasis via induction of smooth muscle relaxation. In cardiovascular diseases (CVDs), endothelial dysfunction with altered vascular reactivity is mostly attributed to decreased NO bioavailability via oxidative stress. However, in several studies, relaxation to NO is only partially restored by exogenous NO donors, suggesting sGC impairment. Conflicting results have been reported regarding the nature of this impairment, ranging from decreased expression of one or both subunits of sGC to heme oxidation. We showed that sGC activity is impaired by thiol S-nitrosation. Recently, angiotensin II (ANG II) chronic treatment, which induces hypertension, was shown to generate nitrosative stress in addition to oxidative stress. We hypothesized that S-nitrosation of sGC occurs in ANG II-induced hypertension, thereby leading to desensitization of sGC to NO hence vascular dysfunction. As expected, ANG II infusion increases blood pressure, aorta remodeling, and protein S-nitrosation. Intravital microscopy indicated that cremaster arterioles are resistant to NO-induced vasodilation in vivo in anesthetized ANG II-treated rats. Concomitantly, NO-induced cGMP production decreases, which correlated with S-nitrosation of sGC in hypertensive rats. This study suggests that S-nitrosation of sGC by ANG II contributes to vascular dysfunction. This was confirmed in vitro by using A7r5 smooth muscle cells infected with adenoviruses expressing sGC or cysteine mutants: ANG II decreases NO-stimulated activity in the wild-type but not in one mutant, C516A. This result indicates that cysteine 516 of sGC mediates ANG II-induced desensitization to NO in cells. nitric oxide resistance; vascular dysfunction; oxidative stress; hypertension SOLUBLE GUANYLYL CYCLASE (sGC) is the main receptor for nitric oxide (NO) and the enzyme responsible for the conversion of GTP into cGMP. As such, the NO-receptor sGC is crucially involved in the physiology of the cardiovascular system by modulating vessel tone. Indeed, mice lacking this receptor are hypertensive (5). Oxidative stress is associated with cardiovascular diseases (CVDs) such as hypertension, atherosclerosis, and diabetes. Most oxidative CVDs are accompanied by endothelial dysfunction and impaired vascular reactivity with decreased NO bioavailability. Nonetheless, it should be pointed out that oxidative stress affects as well the smooth muscle cell (SMC) layers where sGC is expressed. It has been reported that reactive oxygen species (ROS) alter sGC expression and activity (19,25,29). We (21) have previously shown that in vitro and in vivo S-nitrosation of sGC impairs its ability to be activated by NO. In particular, we established that infusion of low therapeutic doses of nitroglycerin in rats for 3 days i...

show abstract

“…Salvia miltiorrhiza (Red sage, Danshen): has been reported to stimulate endothelial NO synthase (eNOS) production and reduced BP in the two-kidney, one-clip (2KIC) renovascular HTN model in hamsters (Kim et al, 2007). …”

Section: Plants With Cardiovascular Effectsmentioning

confidence: 99%

Effect of Tulbaghia violacea on the blood pressure and heart rate in male spontaneously hypertensive Wistar rats

Raji

Mugabo

Obikeze

2012

Journal of Ethnopharmacology

View full text Add to dashboard Cite

Effect of Tulbaghia violacea on the blood pressure and heart rate in male spontaneously hypertensive Wistar rats I. A. RAJITulbaghia violacea Harv. (Alliaceae) is a small bulbous herb which belongs to the family, Alliaceae, most commonly associated with onions and garlic. In South Africa (SA), this herb has been traditionally used in the treatment of various ailments, including fever, colds, asthma, paralysis, hypertension (HTN) and stomach problems. The aim of this study was to evaluate the effect of methanol leaf extracts (MLE) of T. violacea on the blood pressure (BP) and heart rate (HR) in anaesthetized male spontaneously hypertensive rats; and to find out the mechanism(s) by which it acts.The MLE of T. violacea (5 -150 mg/kg), angiotensin I (ang I, 3.1 -100 µg/kg), captopril (10 mg/kg), angiotensin II (ang II, 3.1 -50 µg/kg), losartan (30 mg/kg), phenylephrine T. violacea (60 mg/kg) and captopril (10 mg/kg) were injected intraperitoneally into iii some SHR for 21 days to investigate the chronic effect of these agents on plasma levels of aldosterone. The mean change, the mean of the individual percentage changes and the percentage difference (in mean) observed with each intervention was calculated and statistically analyzed using the Student's t test for significant difference (p < 0.05). The Microsoft Excel software was used for statistical analysis.T. violacea significantly (p < 0.05) reduced the systolic, diastolic, and mean arterial BP;and HR dose-dependently. In a dose-dependent manner, ang I, ang II, phenylephrine significantly (p < 0.05) increased the BP, while propranolol, muscarine and atropine reduced the BP. The increases in BP due to dobutamine were not dose-dependent. In a dose dependent manner, phenylephrine and propranolol reduced the HR, while dobutamine increased the HR. The effect of ang I, ang II, muscarine and atropine on HR were not dose-dependent; with both increases as well as decreases observed with ang I, and II and atropine, while decreases were seen with muscarine. Captopril produced significant (p < 0.05) reduction in BP which were not associated with any change in HR.The co-infusion of ang I with the MLE produced significant (p < 0.05) reduction in BP, which were not associated with significant changes in HR. The co-infusion of ang II with the MLE did not produce any significant changes in BP or HR when compared to the infusion of the standard drug alone. The co-infusion of phenylephrine with the MLE did

show abstract

Endothelial nitric oxide synthase is a molecular vascular target for the Chinese herb Danshen in hypertension

Cited by 68 publications

References 40 publications

Traditional Chinese Medicine Syndromes for Essential Hypertension: A Literature Analysis of 13,272 Patients

Traditional Chinese Medicine Syndromes for Essential Hypertension: A Literature Analysis of 13,272 Patients

Soluble guanylyl cyclase is a target of angiotensin II-induced nitrosative stress in a hypertensive rat model

Effect of Tulbaghia violacea on the blood pressure and heart rate in male spontaneously hypertensive Wistar rats

Contact Info

Product

Resources

About