Endothelium-dependent relaxant effect of thrombocytin, a serine proteinase from Bothrops atrox snake venom, on isolated pig coronary arteries

Glusa, Erika; Brauns, H.; Stocker, Kurt

doi:10.1016/0041-0101(91)90064-x

Cited by 10 publications

(4 citation statements)

References 24 publications

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“…In addition, SVSPs components in D. siamensis venom may act directly on the vasculature to increase systemic vasorelaxation and immediate hypotension via the release of vasoactive mediators. It has been demonstrated that a serine proteinase isolated from the venoms of different snake species can induce vasorelaxation via the release of vasoactive mediators, for example, releasing kinin activities by Bitis arietans venom [50] and the vascular endothelium-derived relaxing factor by Bothrops atrox venom [52]. However, further studies are required to elucidate the function of SVSP from D. siamensis venom more precisely.…”

Section: Comparative Venomics and Cardiovascular Effectsmentioning

confidence: 99%

Comparative compositional and functional venomic profiles among venom specimens from juvenile, subadult and adult Russell’s viper ( Daboia siamensis ): correlation with renal pathophysiology in experimental rabbits

Chaiyabutr¹,

Chanhome²,

Vasaruchapong³

et al. 2022

J. Venom. Anim. Toxins incl. Trop.

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Background: Eastern Russell's viper (Daboia siamensis) is one of the most medically significant snakes responsible for the development of acute renal failure. However, variation of the clinical picture and renal pathophysiology following bites by young and adult D. siamensis have not been elucidated. Methods: In this study, we analyzed the venomic profiles of D. siamensis at different maturation stages of juvenile, subadult and adult groups. The same pooled venom from each group was subjected to enzymatic, electrophoretic and proteomic analysis, including sublethal toxicity (0.1 mg/kg iv.) examined on bodily functions by comparing the venom compositional and functional profiles among venom specimens from juvenile, subadult and adult D. siamensis by correlating them with the renal pathophysiology in experimental rabbits. Results:The comparative studies revealed that juvenile venom possessed higher phospholipase A 2 , metalloproteinase and serine proteinase levels, while subadult and adult venoms contained more L-amino acid oxidase, phosphodiesterase, the Kunitz-type serine protease inhibitor, disintegrin families and endothelial growth factor. An in vivo study revealed that the adult and subadult venoms caused persistent hypotension and bradycardia, while thrombocytopenia was a more characteristic effect of juvenile venom. All venom age groups showed significant reductions in renal hemodynamics and electrolyte excretions. The juvenile venom caused a higher tubulonephrosis lesion score than adult and subadult venoms. Conclusions: The D. siamensis venom shows an ontogenetic shift in its compositions and activities. Renal function alterations after envenomation depend on either the synergistic actions of different venom components or the disproportionate expression between the concentrations of enzymatic and non-enzymatic proteins in each age venom group. The high proportion of enzymatic toxin proteins in the juvenile venom results in greater nephrotoxicity.

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Section: Comparative Venomics and Cardiovascular Effectsmentioning

confidence: 99%

Comparative compositional and functional venomic profiles among venom specimens from juvenile, subadult and adult Russell’s viper ( Daboia siamensis ): correlation with renal pathophysiology in experimental rabbits

Chaiyabutr¹,

Chanhome²,

Vasaruchapong³

et al. 2022

J. Venom. Anim. Toxins incl. Trop.

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“…Like thrombin, thrombin-like toxins can be multifunctional enzymes. There are thrombin-like enzymes reported to have FV activator activity [58], such as Thrombocytin from Bothrops atrox venom, which can also hydrolyze prothrombin, activate FXIII, FVIII and platelets [61], and cause an endothelium-dependent relaxation on arteries [62]. Despite most snake venom thrombin-like enzymes that only hydrolyze fibrinogen and have no effect on the other clotting factors, some thrombin-like toxins have shown activity in nervous and complement systems [25].…”

Section: Thrombin-like Enzymesmentioning

confidence: 99%

Exogenous Procoagulant Factors as Therapeutic and Biotechnological Tools

Chudzinski-Tavassi

Carvalho²,

Andrade³

et al. 2014

J Blood Disord Transfus

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White (2005) listed the snakes of medical importance that affect the hemostatic system. The venom of these reptiles can cause several types of coagulopathy including procoagulant and anticoagulant actions, fibrinogen clotting, fibrinolysis, platelet-activation, prothrombotic and hemorrhagic states [14]. A diversity of toxins from snake venoms has been described: hemorragins, clotting factor activators, clotting

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“…Many studies have reported the responses of the vascular system to thrombin. Thrombin causes vasodilation of the coronary (pig, dog, and human) [1][2][3][4] and pulmonary (newborn pig) arteries 5 and vasoconstriction of the pulmonary (pig) 6 and cerebral (dog) arteries. 7,8 In the porcine renal interlobar artery, thrombin also causes vasorelaxation at low concentrations and vasoconstriction at high concentrations.…”

Section: Introductionmentioning

confidence: 99%

Thrombin-Induced Responses via Protease-Activated Receptor 1 Blocked by the Endothelium on Isolated Porcine Retinal Arterioles

Takahashi

Omae

Ono

et al. 2018

Current Eye Research

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Endothelium-dependent relaxant effect of thrombocytin, a serine proteinase from Bothrops atrox snake venom, on isolated pig coronary arteries

Cited by 10 publications

References 24 publications

Comparative compositional and functional venomic profiles among venom specimens from juvenile, subadult and adult Russell’s viper ( Daboia siamensis ): correlation with renal pathophysiology in experimental rabbits

Comparative compositional and functional venomic profiles among venom specimens from juvenile, subadult and adult Russell’s viper ( Daboia siamensis ): correlation with renal pathophysiology in experimental rabbits

Exogenous Procoagulant Factors as Therapeutic and Biotechnological Tools

Thrombin-Induced Responses via Protease-Activated Receptor 1 Blocked by the Endothelium on Isolated Porcine Retinal Arterioles

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