Endothelium-Mimicking Multifunctional Coating Modified Cardiovascular Stents via a Stepwise Metal-Catechol-(Amine) Surface Engineering Strategy

Yang, Ying; Gao, Peng; Wang, Juan; Tu, Qiufen; Bai, Long; Xiong, Kaiqin; Qiu, Hua; Zhao, Xin; Maitz, Manfred F.; Wang, Huaiyu; Li, Xiangyang; Zhao, Qiang; Xiao, Yin; Huang, Nan; Yang, Zhilu

doi:10.34133/2020/9203906

Cited by 98 publications

(78 citation statements)

References 73 publications

(108 reference statements)

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“…Herein, we report a layer-by-layer grafting strategy to create a biomimetic surface with endothelium-like dual functions on the cardiovascular scaffolds (Figure 1). In this study, the NOgenerating Cu 2+ -chelated DOTA and the typical glycocalyx component HA were sequentially immobilized on plasma polymeric allylamine (PPAm)-coated stents [16] through water-phase amidation. The layer-by-layer grafting strategy enables independent control of the biomolecular density and content on each grafting layer, thus facilitating the adjusting and optimizing surface functions in multicomponent modifications.…”

Section: Introductionmentioning

confidence: 99%

Mimicking the Nitric Oxide‐Releasing and Glycocalyx Functions of Endothelium on Vascular Stent Surfaces

Lyu

Qiu

et al. 2020

Advanced Science

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Endothelium can secrete vasoactive mediators and produce specific extracellular matrix, which contribute jointly to the thromboresistance and regulation of vascular cell behaviors. From a bionic point of view, introducing endothelium‐like functions onto cardiovascular stents represents the most effective means to improve hemocompatibility and reduce late stent restenosis. However, current surface strategies for vascular stents still have limitations, like the lack of multifunctionality, especially the monotony in endothelial‐mimic functions. Herein, a layer‐by‐layer grafting strategy to create endothelium‐like dual‐functional surface on cardiovascular scaffolds is reported. Typically, a nitric oxide (NO, vasoactive mediator)‐generating compound and an endothelial polysaccharide matrix molecule hyaluronan (HA) are sequentially immobilized on allylamine‐plasma‐deposited stents through aqueous amidation. In this case, the stents could be well‐engineered with dual endothelial functions capable of remote and close‐range regulation of the vascular microenvironment. The synergy of NO and endothelial glycocalyx molecules leads to efficient antithrombosis, smooth muscle cell (SMC) inhibition, and perfect endothelial cell (EC)‐compatibility of the stents in vitro. Moreover, the dual‐functional stents show efficient antithrombogenesis ex vivo, rapid endothelialization, and long‐term prevention of restenosis in vivo. Therefore, this study will provide new solutions for not only multicomponent surface functionalization but also the bioengineering of endothelium‐mimic vascular scaffolds with improved clinical outcomes.

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Section: Introductionmentioning

confidence: 99%

Mimicking the Nitric Oxide‐Releasing and Glycocalyx Functions of Endothelium on Vascular Stent Surfaces

Lyu

Qiu

et al. 2020

Advanced Science

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“…In addition, a second-step conjugation with bioactive molecules through amino- or thiol-mediated Michael addition allows a variety of biofunctionalizations. Undoubtedly, mussel-inspired molecular adhesion can provide a potentially universal strategy for surface bioengineering [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

A Versatile Surface Bioengineering Strategy Based on Mussel-Inspired and Bioclickable Peptide Mimic

et al. 2020

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In this work, we present a versatile surface engineering strategy by the combination of mussel adhesive peptide mimicking and bioorthogonal click chemistry. The main idea reflected in this work derived from a novel mussel-inspired peptide mimic with a bioclickable azide group (i.e., DOPA4-azide). Similar to the adhesion mechanism of the mussel foot protein (i.e., covalent/noncovalent comediated surface adhesion), the bioinspired and bioclickable peptide mimic DOPA4-azide enables stable binding on a broad range of materials, such as metallic, inorganic, and organic polymer substrates. In addition to the material universality, the azide residues of DOPA4-azide are also capable of a specific conjugation of dibenzylcyclooctyne- (DBCO-) modified bioactive ligands through bioorthogonal click reaction in a second step. To demonstrate the applicability of this strategy for diversified biofunctionalization, we bioorthogonally conjugated several typical bioactive molecules with DBCO functionalization on different substrates to fabricate functional surfaces which fulfil essential requirements of biomedically used implants. For instance, antibiofouling, antibacterial, and antithrombogenic properties could be easily applied to the relevant biomaterial surfaces, by grafting antifouling polymer, antibacterial peptide, and NO-generating catalyst, respectively. Overall, the novel surface bioengineering strategy has shown broad applicability for both the types of substrate materials and the expected biofunctionalities. Conceivably, the “clean” molecular modification of bioorthogonal chemistry and the universality of mussel-inspired surface adhesion may synergically provide a versatile surface bioengineering strategy for a wide range of biomedical materials.

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“…In addition to the above strategies to regulate the NO flux by using different coating compositions, Chandrawati et al incorporated zinc oxide (ZnO) particles with EPT (Yang et al, 2020c ). ZnO possess innate glutathione peroxidase and glycosidase activities, which allowed ZnO to catalytically decompose both endogenous (RSNOs) and exogenous (β-Gal-NONOate) donors to produce NO at physiological conditions.…”

Section: No-releasing and -Generating Integrating Coatingsmentioning

confidence: 99%

Nitric Oxide-Producing Cardiovascular Stent Coatings for Prevention of Thrombosis and Restenosis

Rao

Bei

Yang

et al. 2020

Front. Bioeng. Biotechnol.

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Cardiovascular stenting is an effective method for treating cardiovascular diseases (CVDs), yet thrombosis and restenosis are the two major clinical complications that often lead to device failure. Nitric oxide (NO) has been proposed as a promising small molecule in improving the clinical performance of cardiovascular stents thanks to its anti-thrombosis and anti-restenosis ability, but its short half-life limits the full use of NO. To produce NO at lesion site with sufficient amount, NO-producing coatings (including NO-releasing and NO-generating coatings) are fashioned. Its releasing strategy is achieved by introducing exogenous NO storage materials like NO donors, while the generating strategy utilizes the in vivo substances such as S-nitrosothiols (RSNOs) to generate NO flux. NO-producing stents are particularly promising in future clinical use due to their ability to store NO resources or to generate large NO flux in a controlled and efficient manner. In this review, we first introduce NO-releasing and-generating coatings for prevention of thrombosis and restenosis. We then discuss the advantages and drawbacks on releasing and generating aspects, where possible further developments are suggested.

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Endothelium-Mimicking Multifunctional Coating Modified Cardiovascular Stents via a Stepwise Metal-Catechol-(Amine) Surface Engineering Strategy

Cited by 98 publications

References 73 publications

Mimicking the Nitric Oxide‐Releasing and Glycocalyx Functions of Endothelium on Vascular Stent Surfaces

Mimicking the Nitric Oxide‐Releasing and Glycocalyx Functions of Endothelium on Vascular Stent Surfaces

A Versatile Surface Bioengineering Strategy Based on Mussel-Inspired and Bioclickable Peptide Mimic

Nitric Oxide-Producing Cardiovascular Stent Coatings for Prevention of Thrombosis and Restenosis

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