Inappropriate farm management practices can lead to increased agricultural inputs and changes in atmospheric greenhouse gas (GHG) emissions, impacting climate change. This study assessed the potential of straw retention to mitigate the negative environmental impact of different cropping systems on the Songnen Plain using the life cycle assessment (LCA) method combined with field survey data. Straw retention (STR) and straw removal (STM) treatments were established in continuous corn (CC) and corn-soybean rotation (CS) systems in a split-plot experiment. The effects of straw retention on the carbon footprint (CF) of cropland under different cropping systems were compared. The CF under CC was 2434.0–2706.9 kg CO 2 ha -1 yr -1 , 49.3%–57.3% higher than that under CS. Nitrogen fertilizer produced the most CO 2 , accounting for 66.2%-80.4% of the CF. The carbon balances of the CC and CS systems with STR were positive, with annual carbon sequestrations of 9632.5 and 2715.9 kg CO 2 ha -1 yr -1 , respectively. The carbon balances of the CC and CS systems with STM was negative, with annual carbon sequestrations of -3589.2 and -3006.2 kg CO 2 ha -1 yr -1 , respectively. This study demonstrates that STR under CC cultivation is an environmentally friendly practice for agricultural production, can help achieve high-yield and low-carbon production in rainfed cropland, and can support the sustainable development of grain production in Northeast China.