Energy Metabolism : Methods in Isolated Smooth Muscle and Methods at Cellular and Subcellular Levels

Stephens, N. L.; Kroeger, Edwin A.; Wrogemann, Klaus

doi:10.1007/978-1-4684-2751-6_31

Cited by 9 publications

(4 citation statements)

References 79 publications

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“…The preparation was incubated in the Tyrode solution for approximately 60 min until the muscle became stabilized. An oxygen consumption was measured by a similar method as described earlier [13] using a Clark-type polarograph electrode connected with a biological oxygen monitor (model 53, YSI).…”

Section: Extraction and Fractionation From The Leaves Of Gymnema Inodmentioning

confidence: 99%

Suppression of Glucose Absorption by Extracts from the Leaves of Gymnema inodorum.

Shimizu

Ozeki

Tanaka

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. Gymnema sylvestre (GS) is one of the Asclepiad strains that grows in South-east Asia. Their therapeutic effects for treating diabetes mellitus, rheumatic arthritis and gout have been well known for a long time. However, the problem is that GS suppresses sweetness and tastes bitter. For this study, we chose Gymnema inodorum (GI) instead of GS, since it has an advantage that it does not suppress sweetness nor is it bitter in taste. In this paper, effects of glucose availability of some saponin fractions (F-I to F-IV) extracted from GI leaves, which were obtained by high-performance liquid chromatography were studied on a high K + -induced contraction of guinea-pig intestinal smooth muscle, O 2 consumption on guinea-pig ileum, glucose-evoked transmural potential difference (∆PD) of guinea-pig everted intestine and blood glucose level in glucose tolerance tests on rats. The extracts of GI leaves suppressed the intestinal smooth muscle contraction, decreased the O 2 consumption, inhibited the glucose evoked-transmural potential, and prevented the blood glucose level. Our studies suggest that the component of GI inhibits the increase in the blood glucose level by interfering with the intestinal glucose absorption process. -KEY WORDS: glucose tolerance test, Gymnema inodorum leaf, muscle contraction, oxygen consumption, transmural potential.

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Section: Extraction and Fractionation From The Leaves Of Gymnema Inodmentioning

confidence: 99%

Suppression of Glucose Absorption by Extracts from the Leaves of Gymnema inodorum.

Shimizu

Ozeki

Tanaka

et al. 1997

The Journal of Veterinary Medical Science

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“…The CK activity of a mitochondrial fraction isolated from taenia caeci was approximately 1 gmol/min/mg mitochondrial protein at 37 ~ C [37,48]. Assuming a mitochondrial content of 5 mg/gwet wt, as estimated in the canine tracheal smooth muscle [63], the Mi-CK activity seems to well match the oxygen consumption rate in the taenia caeci.…”

Section: Physiological Significance Of Ck Compartmentation In Smooth mentioning

confidence: 99%

Compartmentation of ATP synthesis and utilization in smooth muscle: roles of aerobic glycolysis and creatine kinase

Ishida

Riesinger²,

Wallimann³

et al. 1994

Mol Cell Biochem

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The phosphocreatine content of smooth muscle is of similar magnitude to ATP. Thus the function of the creatine kinase system in this tissue cannot simply be regarded as an energy buffer. Thus an understanding of its role in smooth muscle behavior can point to CK function in other systems. From our perspective CK function in smooth muscle is one example of a more general phenomenon, that of the co-localization of ATP synthesis and utilization. In an interesting and analogous fashion distinct glycolytic cascades are also localized in regions of the cell with specialized energy requirements. Similar to CK, glycolytic enzymes are known to be localized on thin filaments, sarcoplasmic reticulum and plasma membrane. In this chapter we will describe the relations between glycolysis and smooth muscle function and compare and contrast to that of the CK system. Our goal is to more fully understand the significance of the compartmentation of distinct pathways for ATP synthesis with specific functions in smooth muscle. This organization of metabolism and function seen most clearly in smooth muscle is likely representative of many other cell types.

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“…Oxygen consumption was measured by a method similar to that described in the paper by Stephens et al (13), using a Clark-type polarograph electrode (YSI) connected to a biological oxygen monitor (YSI model 53).…”

Section: Ratiomentioning

confidence: 99%

A comparative study on the contraction lnduced by high K+/Na+ deficient solution in rat uterus or urinary bladder.

et al. 1988

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Abstract-In the present paper, effects of high K+/Na+ deficient solution on the rat uterus were examined on the mechanical response, wet weight of the tissue or rate of oxygen consumption, and they were compared with those in rat urinary bladder. In the uterus, isosmotic substitution of K+ for Na+ in a physiological saltsolution (PSS) induced a contraction followed by a small sustained contraction, while hyperosmotic addition of KCl to PSS induced a sustained contraction.The hyperosmotic KCl addition increased the rate of oxygen consumption in comparison with that in PSS, and the substituted high K+/Na+ deficient solution decreased it as compared with that in the hyperosmotic KCl addition. Similar results were shown in the urinary bladder.At 120 min after application of the substituted high K+/Na+ deficient solution, the relative wet weight increased in the uterus, but did not change in the urinary bladder.Both the decrease in the developed tension and the increase in the wet weight of the uterus were prevented by the hyperosmotic addition of sucrose.In the urinary bladder, the decreased tension was significantly prevented by the hyperosmotic addition of NaCl to the PSS or substitution of pyruvate or oxalacetate for glucose, whereas it was slightly prevented by the hyperosmotic addition of sucrose.From these results, it is suggested that the decrease of the developed tension in isosmotically substituted high K+/Na+ deficient solution in rat uterus is probably due to cell swelling and that the inhibition of contraction in urinary bladder is mainly caused by the inhibition of glucose utilization by Na+ deficiency in the medium.

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Energy Metabolism : Methods in Isolated Smooth Muscle and Methods at Cellular and Subcellular Levels

Cited by 9 publications

References 79 publications

Suppression of Glucose Absorption by Extracts from the Leaves of Gymnema inodorum.

Suppression of Glucose Absorption by Extracts from the Leaves of Gymnema inodorum.

Compartmentation of ATP synthesis and utilization in smooth muscle: roles of aerobic glycolysis and creatine kinase

A comparative study on the contraction lnduced by high K+/Na+ deficient solution in rat uterus or urinary bladder.

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