Energy transfer pathways in the CAC light-harvesting complex of Rhodomonas salina

Šebelík, Václav; West, Robert G.; Trsková, Eliška Kuthanová; Kaňa, Radek; Polı́vka, Tomáš

doi:10.1016/j.bbabio.2020.148280

Cited by 13 publications

(7 citation statements)

References 48 publications

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“…The Rs PSII-PSI-CAC megacomplex and PsaQ may provide a platform for binding lumen-localized PBPs, and chlorophylls in PsaQ and CACs could ensure the excitation energy harvested by PBPs being transferred to the core, as shown by our Rs PE545-PSII-PSI-CAC megacomplex model. This model is in agreement with an earlier suggestion that PE545 transfers the energy to photosystems through CACs 28,29 . Moreover, our model explains previous data measured by the steady-state and time-resolved fluorescence anisotropy, showing that PBPs funnel the excitation energy to both photosystems with similar efficiency 25,27 .…”

Section: Discussionsupporting

confidence: 93%

Growth phase-dependent reorganization of cryptophyte photosystem I antennae

Zhang,

Si,

et al. 2024

Preprint

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Photosynthetic cryptophytes are eukaryotic algae that utilize membrane-embedded chlorophyll a/c binding proteins (CACs) and lumen-localized phycobiliproteins (PBPs) as their light-harvesting antennae. Cryptophytes go through logarithmic and stationary growth phases, and may adjust their light-harvesting capability according to their particular growth state. How cryptophytes change the type/arrangement of the photosynthetic antenna proteins to regulate their light-harvesting remains unknown. Here we solved four structures of cryptophyte photosystem I (PSI) bound with CACs that show the rearrangement of CACs at different growth phases. We identified a cryptophyte-unique protein, PsaQ, which harbors three chlorophyll molecules. PsaQ specifically binds to the lumenal region of PSI during logarithmic growth phase and may assist the association of PBPs with photosystems and energy transfer from PBPs to photosystems.

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Section: Discussionsupporting

confidence: 93%

Growth phase-dependent reorganization of cryptophyte photosystem I antennae

Zhang,

Si,

et al. 2024

Preprint

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“…The light‐harvesting complex is a cluster of protein and chlorophyll molecules embedded in the thylakoid membrane of plants (Šebelík, West, Trsková, Kaňa, & Polívka, 2020). This complex transfers light energy to the chlorophyll molecules.…”

Section: Discussionmentioning

confidence: 99%

iTRAQ‐based quantitative proteomic analysis ofSargassum fusiformein response to high temperature stress

Liu

Lin

et al. 2020

Aquac. Res.

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Global warming increases seawater temperature, causing high temperature stress to marine organisms, including algae. This study aimed to explore the global proteomic response of Sargassum fusiforme under high temperature stress. Sargassum fusiforme seedlings were cultured in natural seawater for 24 hr and subjected to different temperatures (22°C, control group; 27°C and 32°C, high temperature stress group) for 1, 3, 5 and 7 days. Changes in their membrane lipid peroxidation after high temperature stress were investigated. Proteomic changes in the air bladders of S. fusiforme were analysed using isobaric tags for relative and absolute quantification, along with liquid chromatography-tandem mass spectrometry. Data were analysed using bioinformatics methods. Results showed that high temperature stress destroyed the cell membrane of the air bladders. Further, 28 and 53 differentially expressed proteins (DEPs) were found in the 27°C and 32°C treatment groups respectively. These DEPs were mainly involved in glycolysis, single-organism catabolism, purine nucleoside diphosphate metabolism and carbohydrate catabolism. In addition, DEPs were significantly enriched in 10 pathways, such as glycolytic process, biosynthesis of antibiotics, ribosome, biosynthesis of secondary metabolites and biosynthesis of amino acids. Proteomics analyses indicated that proteins associated with synthesis, folding, degradation, photosynthesis and energy and carbohydrate metabolism are differentially expressed under high temperature stress and normal conditions.

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“…One way phytoplankton can respond to shifts in the spectral environment is by adjusting the ratio of various pigments in response to the spectral environment (Sanfilippo et al, 2019; Sebelik et al, 2020). Known as chromatic acclimation (or chromatic ‘adaptation’), this is a form of reversible phenotypic plasticity where photophysiology is adjusted to maximize light absorption (Bennett & Bogorad, 1973; Engelmann, 1883; Gaidukov, 1903).…”

Section: Introductionmentioning

confidence: 99%

Consequences of light spectra for pigment composition and gene expression in the cryptophyte Rhodomonas salina

Schomaker,

Richardson,

Dudycha

2023

Environmental Microbiology

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Algae with a more diverse suite of pigments can, in principle, exploit a broader swath of the light spectrum through chromatic acclimation, the ability to maximize light capture via plasticity of pigment composition. We grew Rhodomonas salina in wide‐spectrum, red, green, and blue environments and measured how pigment composition differed. We also measured expression of key light‐capture and photosynthesis‐related genes and performed a transcriptome‐wide expression analysis. We observed the highest concentration of phycoerythrin in green light, consistent with chromatic acclimation. Other pigments showed trends inconsistent with chromatic acclimation, possibly due to feedback loops among pigments or high‐energy light acclimation. Expression of some photosynthesis‐related genes was sensitive to spectrum, although expression of most was not. The phycoerythrin α‐subunit was expressed two‐orders of magnitude greater than the β‐subunit even though the peptides are needed in an equimolar ratio. Expression of genes related to chlorophyll‐binding and phycoerythrin concentration were correlated, indicating a potential synthesis relationship. Pigment concentrations and expression of related genes were generally uncorrelated, implying post‐transcriptional regulation of pigments. Overall, most differentially expressed genes were not related to photosynthesis; thus, examining associations between light spectrum and other organismal functions, including sexual reproduction and glycolysis, may be important.

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Energy transfer pathways in the CAC light-harvesting complex of Rhodomonas salina

Cited by 13 publications

References 48 publications

Growth phase-dependent reorganization of cryptophyte photosystem I antennae

Growth phase-dependent reorganization of cryptophyte photosystem I antennae

iTRAQ‐based quantitative proteomic analysis ofSargassum fusiformein response to high temperature stress

Consequences of light spectra for pigment composition and gene expression in the cryptophyte Rhodomonas salina

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