Engineered Cartilage, Bone, Joints, and Menisci

Glowacki, Julie

doi:10.1159/000047895

Cited by 27 publications

(7 citation statements)

References 25 publications

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“…Other potential applications are for replacement of damaged intervertebral discs, knee menisci, anatomical disorders of the temporomandibular joint, and severe deformities of the midface and ears. 14,15 Cartilage is well-suited for tissue engineering because it lacks a blood supply, is nourished by diffusion, and has a low cell-to-matrix ratio. Although there is some organization of zones, it is composed of a single cell type, the chondrocyte.…”

Section: Tissue Engineering For Musculoskeletal Deficienciesmentioning

confidence: 99%

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Collagen scaffolds for tissue engineering

2007

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There are two major approaches to tissue engineering for regeneration of tissues and organs. One involves cell‐free materials and/or factors and one involves delivering cells to contribute to the regeneraion process. Of the many scaffold materials being investigated, collagen type I, with selective removal of its telopeptides, has been shown to have many advantageous features for both of these approaches. Highly porous collagen lattice sponges have been used to support in vitro growth of many types of tissues. Use of bioreactors to control in vitro perfusion of medium and to apply hydrostatic fluid pressure has been shown to enhance histogenesis in collagen scaffolds. Collagen sponges have also been developed to contain differentiating‐inducing materials like demineralized bone to stimulate differentiation of cartilage tissue both in vitro and in vivo. © 2007 Wiley Periodicals, Inc. Biopolymers 89: 338–344, 2008.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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Section: Tissue Engineering For Musculoskeletal Deficienciesmentioning

confidence: 99%

“…Bone tissue engineering seeks to augment or accelerate bone formation to meet functional demands. 15,19 To do so, there are additional demands beyond those for cartilage because bone is a more rigid tissue and is vascularized.…”

Section: Tissue Engineering For Musculoskeletal Deficienciesmentioning

confidence: 99%

Collagen scaffolds for tissue engineering

2007

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“…For bone regeneration, slowly degrading biomaterials maintaining implant integrity following implantation while continually transferring the load-bearing burden to the developing and functional host tissue are considered ideal [20–22]. Many natural and synthetic biomaterials have been explored for load-bearing applications in vivo .…”

Section: Introductionmentioning

confidence: 99%

Remodeling of tissue-engineered bone structures in vivo

Hofmann

Hilbe

Fajardo

et al. 2013

European Journal of Pharmaceutics and Biopharmaceutics

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Implant design for bone regeneration is expected to be optimized when implant structures resemble the anatomical situation of the defect site. We tested the validity of this hypothesis by exploring the feasibility of generating different in vitro engineered bone-like structures originating from porous silk fibroin scaffolds decorated with RGD sequences (SF-RGD), seeded with human mesenchymal stem cells (hMSC). Scaffolds with small (106 – 212 μm), medium (212 – 300 μm) and large pore diameter ranges (300 – 425 μm) were seeded with hMSC and subsequently differentiated in vitro into bone-like tissue resembling initial scaffold geometries and featuring bone-like structures. Eight weeks after implantation into calvarial defects in mice, the in vitro engineered bone-like tissues had remodeled into bone featuring different proportions of woven/lamellar bone bridging the defects. Regardless of pore diameter all implants integrated well, vascularization was advanced and, bone marrow ingrowth had started. Ultimately, in this defect model, the geometry of the in vitro generated tissue-engineered bone structure, trabecular- or plate-like, had no significant impact on the healing of the defect, owing to an efficient remodeling of its structure after implantation.

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“…As more information is obtained about the molecular pathways that mediate joint cavitation during embryogenesis, 6,22 it may be possible to have greater control of the elements of engineered musculoskeletal tissues. 23,24 …”

Section: Discussionmentioning

confidence: 99%

Engineering a Joint: A Chimeric Construct with Bovine Chondrocytes in a Devitalized Chick Knee

Zaleske

Peretti²,

Allemann³

et al. 2003

Tissue Engineering

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This study assessed the feasibility of a devitalized knee as a scaffold for an engineered chimeric joint. Embryonic chick knees (19 days old), devitalized by lyophilization or multiple freeze-thaw cycles, were tested as scaffolds for repopulation with bovine articular chondrocytes (bACs). bACs were seeded into porous three-dimensional collagen sponges and were cultured for 1 day before fabrication of chimeric constructs. A pair of cell-seeded sponges was inserted into the joint space to contact preshaved articular surfaces. In some constructs, a sterile membrane of expanded polytetrafluoroethylene (ePTFE) was inserted between the collagen sponges. Histologic analysis showed that at 1 week, sponges with bACs were adherent to the shaved articular surfaces of the joint with accumulation of metachromatic extracellular matrix. Penetration of bACs and neomatrix into the devitalized matrix appeared to begin in preexistent epiphyseal canals and was observed to some extent in all specimens. Membranes of ePTFE maintained a joint space at 2 and 3 weeks, whereas there was fusion across the two sponges in many specimens lacking the membrane. Gene expression analysis demonstrated that lyophilization, but not multiple freeze-thaw cycles, completely devitalized the chick knees. These studies identified several design parameters crucial for successful engineering of a chimeric joint.

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Engineered Cartilage, Bone, Joints, and Menisci

Cited by 27 publications

References 25 publications

Collagen scaffolds for tissue engineering

Collagen scaffolds for tissue engineering

Remodeling of tissue-engineered bone structures in vivo

Engineering a Joint: A Chimeric Construct with Bovine Chondrocytes in a Devitalized Chick Knee

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