Engineered Geobacillus lipolytic enzymes – Attractive polyesterases that degrade polycaprolactones and simultaneously produce esters

Malunavicius, Vilius; Padaiga, Antanas; Stankeviciute, Jonita; Pakalniskis, Andrius; Gudiukaite, Renata

doi:10.1016/j.ijbiomac.2023.127656

Cited by 2 publications

(1 citation statement)

References 130 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the different bacterial genera identified in the community (Fig. 4 A), the genus Bacillus (9% relative abundance) [ 76 , 77 ] and Geobacillus (2%) [ 78 ] hav been previously associated with PCL biodegradation.…”

Section: Resultsmentioning

confidence: 99%

Microplastics Biodegradation by Estuarine and Landfill Microbiomes

Pires,

Costa,

Barbosa

et al. 2024

Microb Ecol

View full text Add to dashboard Cite

Plastic pollution poses a worldwide environmental challenge, affecting wildlife and human health. Assessing the biodegradation capabilities of natural microbiomes in environments contaminated with microplastics is crucial for mitigating the effects of plastic pollution. In this work, we evaluated the potential of landfill leachate (LL) and estuarine sediments (ES) to biodegrade polyethylene (PE), polyethylene terephthalate (PET), and polycaprolactone (PCL), under aerobic, anaerobic, thermophilic, and mesophilic conditions. PCL underwent extensive aerobic biodegradation with LL (99 ± 7%) and ES (78 ± 3%) within 50–60 days. Under anaerobic conditions, LL degraded 87 ± 19% of PCL in 60 days, whereas ES showed minimal biodegradation (3 ± 0.3%). PE and PET showed no notable degradation. Metataxonomics results (16S rRNA sequencing) revealed the presence of highly abundant thermophilic microorganisms assigned to Coprothermobacter sp. (6.8% and 28% relative abundance in anaerobic and aerobic incubations, respectively). Coprothermobacter spp. contain genes encoding two enzymes, an esterase and a thermostable monoacylglycerol lipase, that can potentially catalyze PCL hydrolysis. These results suggest that Coprothermobacter sp. may be pivotal in landfill leachate microbiomes for thermophilic PCL biodegradation across varying conditions. The anaerobic microbial community was dominated by hydrogenotrophic methanogens assigned to Methanothermobacter sp. (21%), pointing at possible syntrophic interactions with Coprothermobacter sp. (a H2-producer) during PCL biodegradation. In the aerobic experiments, fungi dominated the eukaryotic microbial community (e.g., Exophiala (41%), Penicillium (17%), and Mucor (18%)), suggesting that aerobic PCL biodegradation by LL involves collaboration between fungi and bacteria. Our findings bring insights on the microbial communities and microbial interactions mediating plastic biodegradation, offering valuable perspectives for plastic pollution mitigation.

show abstract

Section: Resultsmentioning

confidence: 99%

Microplastics Biodegradation by Estuarine and Landfill Microbiomes

Pires,

Costa,

Barbosa

et al. 2024

Microb Ecol

View full text Add to dashboard Cite

show abstract

Solvent Tolerance Improvement of Lipases Enhanced Their Applications: State of the Art

Chen,

Jin,

Nian

et al. 2024

Molecules

View full text Add to dashboard Cite

Lipases, crucial catalysts in biochemical synthesis, find extensive applications across industries such as food, medicine, and cosmetics. The efficiency of lipase-catalyzed reactions is significantly influenced by the choice of solvents. Polar organic solvents often result in a decrease, or even loss, of lipase activity. Conversely, nonpolar organic solvents induce excessive rigidity in lipases, thereby affecting their activity. While the advent of new solvents like ionic liquids and deep eutectic solvents has somewhat improved the activity and stability of lipases, it fails to address the fundamental issue of lipases’ poor solvent tolerance. Hence, the rational design of lipases for enhanced solvent tolerance can significantly boost their industrial performance. This review provides a comprehensive summary of the structural characteristics and properties of lipases in various solvent systems and emphasizes various strategies of protein engineering for non-aqueous media to improve lipases’ solvent tolerance. This study provides a theoretical foundation for further enhancing the solvent tolerance and industrial properties of lipases.

show abstract

Engineered Geobacillus lipolytic enzymes – Attractive polyesterases that degrade polycaprolactones and simultaneously produce esters

Cited by 2 publications

References 130 publications

Microplastics Biodegradation by Estuarine and Landfill Microbiomes

Microplastics Biodegradation by Estuarine and Landfill Microbiomes

Solvent Tolerance Improvement of Lipases Enhanced Their Applications: State of the Art

Contact Info

Product

Resources

About