Engineered mesenchymal stem cell-derived exosomes with high CXCR4 levels for targeted siRNA gene therapy against cancer

Cui, Daxiang; Xu, Shuyue; Fan, Junyi; Xue, Cuili; Lü, Yi; Liu, Bin; Li, Can

doi:10.1039/d1nr08170e

Cited by 33 publications

(23 citation statements)

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“…In recent years, research on nonviral gene delivery has become popular. From the nanoscale (e.g., liposomes ( Wang et al, 2021 ; Yu et al, 2022 ), complex micelles ( Yoshinaga et al, 2021 ; Yoshinaga et al, 2022 ), and exosomes ( Cui et al, 2022 ; Xu et al, 2022 )) to the micron scale (such as microspheres ( McMillan et al, 2018 ; Hinkelmann et al, 2022 )) or combined forms of nanoparticles and microspheres ( Feng et al, 2017 ; Feng et al, 2020 ; Chang et al, 2022 ), more options for nonviral vectors have been provided. Importantly, genetic factors contribute to the worsening of IVDD, and gene therapy approaches are expected to improve the poor genetic cascade regulation of IVD.…”

Section: Application Of Microsphere-based Delivery Systems In Ivddmentioning

confidence: 99%

New Hope for Treating Intervertebral Disc Degeneration: Microsphere-Based Delivery System

Guo

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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Intervertebral disc (IVD) degeneration (IVDD) has been considered the dominant factor in low back pain (LBP), and its etiological mechanisms are complex and not yet fully elucidated. To date, the treatment of IVDD has mainly focused on relieving clinical symptoms and cannot fundamentally solve the problem. Recently, a novel microsphere-based therapeutic strategy has held promise for IVD regeneration and has yielded encouraging results with in vitro experiments and animal models. With excellent injectability, biocompatibility, and biodegradability, this microsphere carrier allows for targeted delivery and controlled release of drugs, gene regulatory sequences, and other bioactive substances and supports cell implantation and directed differentiation, aiming to improve the disease state of IVD at the source. This review discusses the possible mechanisms of IVDD and the limitations of current therapies, focusing on the application of microsphere delivery systems in IVDD, including targeted delivery of active substances and drugs, cellular therapy, and gene therapy, and attempts to provide a new understanding for the treatment of IVDD.

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Section: Application Of Microsphere-based Delivery Systems In Ivddmentioning

confidence: 99%

New Hope for Treating Intervertebral Disc Degeneration: Microsphere-Based Delivery System

Guo

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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“…By encapsulating si-survivin by electroporation, MSC EVs expressing CXC chemokine receptor type 4 (CXCR4) can specifically bind to the highly expressed stromal cell-derived factor-1 (SDF-1) on the tumor surface, reach the tumor site, knock down the survivin gene, and achieve a tumor-killing effect [ 120 ]. KRAS G12D , the most common oncogene KRAS mutation, has received extensive attention as a promising target for the treatment of solid tumors.…”

Section: Nucleic Acid Drugs Delivered By Evs For Cancer Therapymentioning

confidence: 99%

Extracellular Vesicles as Delivery Vehicles for Therapeutic Nucleic Acids in Cancer Gene Therapy: Progress and Challenges

Wang

Zhu

et al. 2022

Pharmaceutics

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Extracellular vesicles (EVs) are nanoscale vesicles secreted by most types of cells as natural vehicles to transfer molecular information between cells. Due to their low toxicity and high biocompatibility, EVs have attracted increasing attention as drug delivery systems. Many studies have demonstrated that EV-loaded nucleic acids, including RNA-based nucleic acid drugs and CRISPR/Cas gene-editing systems, can alter gene expressions and functions of recipient cells for cancer gene therapy. Here in this review, we discuss the advantages and challenges of EV-based nucleic acid delivery systems in cancer therapy. We summarize the techniques and methods to increase EV yield, enhance nucleic acid loading efficiency, extend circulation time, and improve targeted delivery, as well as their applications in gene therapy and combination with other cancer therapies. Finally, we discuss the current status, challenges, and prospects of EVs as a therapeutic tool for the clinical application of nucleic acid drugs.

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“…As standard EVs, exosomes seem like a good strategy for bone regeneration therapy (60,61). Compared to direct autologous transplantation of cells, treatment based on exosomes has benefits such as accurate targeting, stability, low immunogenicity, quickly crossing biological barriers, and low immune reaction (62,63). The use of hADSCs-Exos for regenerative medicine and tissue engineering is a rich field of research, which is stated by a large number of studies supporting the efficiency of hADSCs-Exos on tissue repair and wound healing in many physiological systems (64).…”

Section: Exosomes Secreted By Cells and Promote Osteogenesismentioning

confidence: 99%

Exosomes as Biochemistry Tools for Stem Cell Differentiation: A Novel Cell-Based Treatment for Diseases

Azandeh

Nejad

Karimi³

et al. 2022

Jentashapir J Cell Mol Biol

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: For tissue engineering and clinical translation strategies, it is essential to have a reliable and safe lineage-specific differentiation of stem cells. To deal with several problems caused by growth factor delivery systems and growth factors, exosomes have been used as biomimetic tools to trigger the differentiation of stem cells. It is believed that cell type-specific exosomes can induce lineage-specific differentiation of stem cells. Exosomes trigger cell viability, cell proliferation and differentiation, embryonic implantation, and migration. They have been used successfully in regenerative medicine, such as liver fibrosis, renal diseases, cardiac ischemia, stroke, and skin injuries. The findings highlighted the necessity to take into account the condition and source of exosome donor cells before selecting them for therapeutic use.

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Engineered mesenchymal stem cell-derived exosomes with high CXCR4 levels for targeted siRNA gene therapy against cancer

Abstract: Gene therapy has been used in a variety of diseases and shows brilliant anti-cancer or cancer suppression effects. Gene therapy is gradually evolving as the most compelling frontier hotspot in...

Cited by 33 publications

References 50 publications

New Hope for Treating Intervertebral Disc Degeneration: Microsphere-Based Delivery System

New Hope for Treating Intervertebral Disc Degeneration: Microsphere-Based Delivery System

Extracellular Vesicles as Delivery Vehicles for Therapeutic Nucleic Acids in Cancer Gene Therapy: Progress and Challenges

Exosomes as Biochemistry Tools for Stem Cell Differentiation: A Novel Cell-Based Treatment for Diseases

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