Engineered versus hybrid cellular vesicles as efficient drug delivery systems: a comparative study with brain targeted vesicles

Kannavou, Maria; Marazioti, Antonia; Stathopoulos, Georgios T.; Antimisiaris, Sophia G.

doi:10.1007/s13346-021-00900-1

Cited by 10 publications

(13 citation statements)

References 41 publications

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“…To quantify the cellular uptake of DOX, all imaging and processing settings were kept constant and the relative fluorescence intensities (FIs) were calculated with Image J (software version 1.8.0) according to the methodology contributed by Luke Hammond (QBI, University of Queensland, Australia) in the open lab book, as described in detail before 70 . https://theolb.readthedocs.io/en/latest/imaging/measuring-cell-fluorescence-using-imagej.html .…”

Section: Methodsmentioning

confidence: 99%

Synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells

Pefani-Antimisiari

Athanasopoulos

Marazioti

et al. 2021

Sci Rep

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The aim of the present study was to investigate combined effects of cold atmospheric plasma (CAP) and the chemotherapeutic drug doxorubicin (DOX) on murine and human melanoma cells, and normal cells. In addition to free drug, the combination of CAP with a liposomal drug (DOX-LIP) was also studied for the first time. Thiazolyl blue tetrazolium bromide (MTT) and Trypan Blue exclusion assays were used to evaluate cell viability; the mechanism of cell death was evaluated by flow cytometry. Combined treatment effects on the clonogenic capability of melanoma cells, was also tested with soft agar colony formation assay. Furthermore the effect of CAP on the cellular uptake of DOX or DOX-LIP was examined. Results showed a strong synergistic effect of CAP and DOX or DOX-LIP on selectively decreasing cell viability of melanoma cells. CAP accelerated the apoptotic effect of DOX (or DOX-LIP) and dramatically reduced the aggressiveness of melanoma cells, as the combination treatment significantly decreased their anchorage independent growth. Moreover, CAP did not result in increased cellular uptake of DOX under the present experimental conditions. In conclusion, CAP facilitates DOX cytotoxic effects on melanoma cells, and affects their metastatic potential by reducing their clonogenicity, as shown for the first time.

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Section: Methodsmentioning

confidence: 99%

Synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells

Pefani-Antimisiari

Athanasopoulos

Marazioti

et al. 2021

Sci Rep

Self Cite

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“…• Simple & Fast • Impairment of drug activity (Sato et al, 2016;Lv et al, 2020;Cheng et al, 2021;Kannavou et al, 2021;Singh et al, 2021) • Relatively high efficiency • Disruption of EVs membrane…”

Section: Methodsmentioning

confidence: 99%

Research progress in membrane fusion-based hybrid exosomes for drug delivery systems

Liu

Yang

Liu

et al. 2022

Front. Bioeng. Biotechnol.

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Liposomes are the earliest and most widely used nanoparticles for targeted drug delivery. Exosomes are nanosized membrane-bound particles and important mediators of intercellular communication. Combining liposomes and exosomes using various membrane fusion methods gives rise to a novel potential drug delivery system called membrane fusion-based hybrid exosomes (MFHE). These novel MFHEs not only exhibit potential advantageous features, such as high drug loading rate and targeted cellular uptake via surface modification, but are also endowed with high biocompatibility and low immunogenicity. Here, we provide an overview of MFHEs’ various preparation methods, characterization strategies, and their applications for disease treatment and scientific research.

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“…Thus, the affinity of ligands that are a component of the exosomal membrane proteome and lipids to specific receptors, and its physical attributes of size and surface charge allow it to move across biological barriers and entangle/trap in tightly packed tissues with poor drainage such as the tumor matrix. For e.g., exosomes isolated from the brain derived endothelial cells (hCMEC/D3 and bEnd.3 cells) have been shown to cross the BBB and target the brain after intravenous administration ( Wu et al, 2021a ; Kannavou et al, 2021 ). Plasma exosomes which are inherently derived from multiple tissues have also been used for targeted delivery towards the brain and improving drug bioavailability ( Qi et al, 2020 ).…”

Section: Programming Exosomes For Modulating Its Biodistribution In Vivomentioning

confidence: 99%

“…For example, intranasal and/or intracerebral administration of exosomes could be utilized to circumvent other organs and maximize exosomal delivery to the brain ( Perets et al, 2018 ). Biomimetic vesicles that are a hybrid of exosomes and exogenous lipids and polymers have also been shown to possess a superior pharmacokinetic profile ( Wu et al, 2021a ; Kannavou et al, 2021 ).…”

Section: Programming Exosomes For Modulating Its Biodistribution In Vivomentioning

confidence: 99%

“…This method did not affect exosome morphology, size distribution, or protein composition and increased the specific binding of exosomes to EGFR-overexpressing tumor cells. However, inconsistent results with PEGylation have also been reported and it might not always ensure an improvement in circulation for other types of exosomes ( Kannavou et al, 2021 ).…”

Section: Programming Exosomes For Modulating Its Biodistribution In Vivomentioning

confidence: 99%

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Exosomes: Biological Pharmaceutical Nanovectors for Theranostics

Thomas

Kim

Pauletti

et al. 2022

Front. Bioeng. Biotechnol.

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Exosomes are natural cell-derived nanovesicles of endocytic origin that enable cellular crosstalk by transferring encapsulated molecular cargos across biological barriers, thereby holding significantly complex implications in the etiology and progression of diverse disease states. Consequently, the development of exosomes-based nano-theranostic strategies has received immense consideration for advancing therapeutic interventions and disease prognosis. Their favorable biopharmaceutical properties make exosomes a unique nanoparticulate carrier for pharmaceutical drug delivery. This review provides an update on the contemporary strategies utilizing exosomes for theranostic applications in nanomedicine. In addition, we provide a synopsis of exosomal features and insights into strategic modifications that control in vivo biodistribution. We further discuss their opportunities, merits and pitfalls for cell/tissue targeted drug delivery in personalized nanotherapy.

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Engineered versus hybrid cellular vesicles as efficient drug delivery systems: a comparative study with brain targeted vesicles

Cited by 10 publications

References 41 publications

Synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells

Synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells

Research progress in membrane fusion-based hybrid exosomes for drug delivery systems

Exosomes: Biological Pharmaceutical Nanovectors for Theranostics

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