Engineering a heterologously expressed fructosyltransferase from Aspergillus oryzae N74 in Komagataella phaffii (Pichia pastoris) for kestose production

Alvarado-Obando, Manuela; Contreras, Nicolás; León, Diana Carolina Rubio; Botero, Lina M.; Beltran, Laura; Suescún-Díaz, Daniel; Rodríguez-López, Alexander; Reyes, Luis H.; Alméciga-Díaz, Carlos J.; Sánchez, Oscar F.

doi:10.1016/j.nbt.2022.02.005

Cited by 7 publications

(2 citation statements)

References 42 publications

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“…The yield of A. oryzae NCH-42 was low at 20 • C, while the yields of GlcN were high at 25 • C and 30 • C [93]. Additionally, a study using A. oryzae to produce fructo-oligosaccharides revealed that the enhanced activity of mutant V242E is not affected by reaction temperature or other environmental factors [107]. Therefore, not all secondary metabolites are sensitive to temperature, and there are different mechanisms in response to temperature stress.…”

Section: The Impact Of Temperaturementioning

confidence: 99%

Strategies for the Enhancement of Secondary Metabolite Production via Biosynthesis Gene Cluster Regulation in Aspergillus oryzae

Jia,

Song,

et al. 2024

JoF

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The filamentous fungus Aspergillus oryzae (A. oryzae) has been extensively used for the biosynthesis of numerous secondary metabolites with significant applications in agriculture and food and medical industries, among others. However, the identification and functional prediction of metabolites through genome mining in A. oryzae are hindered by the complex regulatory mechanisms of secondary metabolite biosynthesis and the inactivity of most of the biosynthetic gene clusters involved. The global regulatory factors, pathway-specific regulatory factors, epigenetics, and environmental signals significantly impact the production of secondary metabolites, indicating that appropriate gene-level modulations are expected to promote the biosynthesis of secondary metabolites in A. oryzae. This review mainly focuses on illuminating the molecular regulatory mechanisms for the activation of potentially unexpressed pathways, possibly revealing the effects of transcriptional, epigenetic, and environmental signal regulation. By gaining a comprehensive understanding of the regulatory mechanisms of secondary metabolite biosynthesis, strategies can be developed to enhance the production and utilization of these metabolites, and potential functions can be fully exploited.

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Section: The Impact Of Temperaturementioning

confidence: 99%

Strategies for the Enhancement of Secondary Metabolite Production via Biosynthesis Gene Cluster Regulation in Aspergillus oryzae

Jia,

Song,

et al. 2024

JoF

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“…The development of bioinformatics tools have provided a better understanding of the catalytic mechanism of different FTases and enabled their in silico evaluation as efficient FOS producers [23,26,27]. Very recently, some authors combined the results of molecular modeling with site-directed mutagenesis and enzyme overproduction [23,28]. Xia et al developed an FTase with an about six times higher specific activity level than that of the wild-type enzyme and improved affinity as well as thermal stability [23].…”

Section: Introductionmentioning

confidence: 99%

Production of Fructooligosaccharides Using a Commercial Heterologously Expressed Aspergillus sp. Fructosyltransferase

Karkeszová

Polakovič

2023

Catalysts

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The catalytic properties of Seqenzym® FT, a fungal fructosyltransferase heterologously expressed in yeasts, were investigated at a temperature of 55 °C and pH 5.5. The initial rate measurements showed that the transfructosylation rate was only slightly inhibited by sucrose above the concentration of 1.5 M. A rather low level of hydrolytic side activity was observed even at sucrose concentrations as low as 0.25 M. In progress curve experiments, the mass yield of fructooligosaccharides (FOS) reached a maximum value of 57% at this sucrose concentration, although it dropped to about 35% later on. At high initial sucrose concentrations up to 2 M, the FOS yield reached a maximum value of approximately 63% at a sucrose conversion of approximately 90%. Although neither the yield nor the conversion changed much later on, the progress of the reaction was manifested by the gradual depletion of shorter chain FOS, 1-kestose and nystose, and the accumulation of 1-β-fructofuranosyl nystose. At initial sucrose concentrations of 2 M, the degree of polymerization expressed through the number of fructosyl units grew from 2.3 at a conversion degree of 87% to 3.1 at a conversion degree of 94%. Compared to other commercial preparations, Seqenzym® FT can better produce FOS with a higher degree of polymerization.

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Fructooligosaccharides: Production by recombinant fructosyltransferase from Festuca arundinacea in a continuous reactor and kinetic modeling profile

Jaswal,

Elangovan,

Mishra

2024

Carbohydrate Polymer Technologies and Applications

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Engineering a heterologously expressed fructosyltransferase from Aspergillus oryzae N74 in Komagataella phaffii (Pichia pastoris) for kestose production

Cited by 7 publications

References 42 publications

Strategies for the Enhancement of Secondary Metabolite Production via Biosynthesis Gene Cluster Regulation in Aspergillus oryzae

Strategies for the Enhancement of Secondary Metabolite Production via Biosynthesis Gene Cluster Regulation in Aspergillus oryzae

Production of Fructooligosaccharides Using a Commercial Heterologously Expressed Aspergillus sp. Fructosyltransferase

Fructooligosaccharides: Production by recombinant fructosyltransferase from Festuca arundinacea in a continuous reactor and kinetic modeling profile

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