Engineering a mucin coating to promote osteogenic differentiation of BMSCs in vitro and bone formation in vivo through the Wnt/β-catenin pathway

Bai, Zhibiao; Hu, Kai; Shou, Zeyu; Zhou, Han; Yu, Jiahuan; Meng, Hongming; Chen, Liangyan; Yu, Tiantian; Lu, Ruofei; Li, Na; Chen, Chun

doi:10.1016/j.colsurfb.2022.113000

Cited by 5 publications

(3 citation statements)

References 52 publications

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“…Comparing the fold change with day 3 to day 6 and day 3 to day 28 we could observe similar data with RNA sequencing that mucin is getting upregulated in day 6 compared to day 28 (Figure 1e) and after having clear idea at the genetic level we validated the importance of mucin protein during bone differentiation. [ 14,18 ] Magnetic bead based pull down of mucin protein was done using protein G beads from 3 days and 14 days differentiated MC3T3‐E1 cells (mouse origin), from which we could confirm the role of mucin during bone differentiation (Figure S2, Supporting Information). We decided to take mouse into consideration because of the similarity in sequences between human, mouse and bovine (Figure S1, Supporting Information and table ST1) which showed about 50–80% similarity in sequences.…”

Section: Resultsmentioning

confidence: 87%

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Glycoprotein Injectable Hydrogels Promote Accelerated Bone Regeneration through Angiogenesis and Innervation

Barik,

Shyamal,

Das

et al. 2023

Adv Healthcare Materials

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Glycoproteins are gaining prominence as multifunctional biomaterials. The study reports development of glycoprotein mucin as biomaterial promoting bone regeneration. Mucin 1 deletion has resulted in stiffer femoral bones with scarce presence of osteoblasts in trabecular linings and its role has been established in determining bone mass and mineralization. Limited information about its structure limited its processability, exploration as biomaterial, which we have successfully overcome in this study. We report the role of mucin in ECM (extracellular cellular matrix) formation validated by RNA sequencing analysis of human bone marrow derived mesenchymal stem cells. The structure and stability of mucins is dependent on the presence of glycans in its structure. A thermosensitive hydrogel acquired from thermosensitive PNIPAM‐modified mucin and collagen is developed. The hydrogel demonstrates porous structure and mechanical strength. Newly formed bone tissue was observed at 8 weeks post‐implantation in the hydrogel treated groups. The formation of blood vessels, nerves and bone is observed with upregulation of angiopoietin, neurofilament heavy chain and osteoadherin respectively in rat calvarial defects. The outcome demonstrates that the thermosensitive injectable hydrogel accelerated repair and healing in calvarial bone defects making it a promising biodegradable biomaterial capable of regenerating bone by promoting angiogenesis and innervation.This article is protected by copyright. All rights reserved

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Section: Resultsmentioning

confidence: 87%

“…More recently, mucin has been studied to play role in ECM of bone and aiding in bone mineralization. [29,30,31] Mucins are composed of glycosaminoglycan (GAG) residues covalently bound to the protein core. The glycoproteins get attached to the matrix leading to the initiation of matrix formation and eventually mineralization of bones.…”

Section: Discussionmentioning

confidence: 99%

Glycoprotein Injectable Hydrogels Promote Accelerated Bone Regeneration through Angiogenesis and Innervation

Barik,

Shyamal,

Das

et al. 2023

Adv Healthcare Materials

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“…Many proteins have been used to construct LbL functional coatings with polyphenols, e.g., gelatin has been assembled with Tannic acid (TA) to obtain antioxidant LbL coatings, 23 collagen has been assembled with TA to obtain antimicrobial LbL lms, 24 and LbL coatings have been assembled with mucin and procyanidin and exhibited excellent osteogenic differentiation of bone marrow mesenchymal stem cells. 25 Recently, we have used the interaction between lysozyme and TA to construct LbL coatings that possess good antioxidant properties. 26 Although the driving force between the lysozyme and TA is the enthalpy change due to noncovalent interactions, speci c noncovalent interaction types and contributions remain unexplained, which is attributed to high molecular weights, complex structures, and unclear interaction regions of proteins.…”

Section: Introductionmentioning

confidence: 99%

Facile Universal Strategy of Presenting Multifunctional Short Peptides for Customizing Desired Surfaces

Lu,

Zhao,

Huo

et al. 2024

Preprint

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Interfacial properties determine biomaterial performances, such as cell adhesion, signal exchange, and biomineralization, which affect the tissue repair cycle and efficiency of clinical applications. Peptides, as short protein sequences that have defined functionalities, are highly stable and easy to synthesize and have enormous potential to reshape interfacial properties. However, the lack of a universal strategy for presenting peptides on various substrates substantially hinders the application of peptides. In this study, we report a facile and universal strategy for customizing desired interfacial functionalities by a well-known layer-by-layer (LbL) technique through the assembly polyphenols with positively charged short peptide-coupling functional sequences. Polyphenol–peptide interactions were elucidated in detail by assembling polyphenols and peptides possessing different characteristics (charged, uncharged, hydrophobic, and sequence length) in combination with molecular dynamics simulations, and isothermal titration calorimetry further revealed the favorable enthalpy change due to electrostatic interactions is the main driving force for assembling peptides with polyphenols. LbL coatings assembled from polyphenols and positively charged peptides exhibited good substrate generalization, stability, cell proliferation, and antioxidant properties, when prepared as hollow capsules by sacrificing the template, exhibited significant pH and ultrasound stimulation responses, which could be suitable candidates for drug carriers. Most importantly, the LbL assembly strategy of positively charged peptides could be utilized to present various functional molecules (such as arginyl–glycyl–aspartic acid (RGD), a cell adhesion motif; CM15, an antibacterial peptide; and PEG, an antifouling surface) on various substrates for customizing desired surfaces. This study not only provides new insights into the understanding and regulation of interactions between proteins/peptides and polyphenols but also paves the way toward the interfacial functionalization of biomaterials.

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A strategy of de novo peptides for customizing supramolecular self-assembly coating with desired biological functionalities

Li,

Shou,

Liu

et al. 2024

Chemical Engineering Journal

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Engineering a mucin coating to promote osteogenic differentiation of BMSCs in vitro and bone formation in vivo through the Wnt/β-catenin pathway

Cited by 5 publications

References 52 publications

Glycoprotein Injectable Hydrogels Promote Accelerated Bone Regeneration through Angiogenesis and Innervation

Glycoprotein Injectable Hydrogels Promote Accelerated Bone Regeneration through Angiogenesis and Innervation

Facile Universal Strategy of Presenting Multifunctional Short Peptides for Customizing Desired Surfaces

A strategy of de novo peptides for customizing supramolecular self-assembly coating with desired biological functionalities

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