Engineering apomixis in crops

Mahlandt, Alexander; Singh, Dipesh Kumar; Mercier, Raphaël

doi:10.1007/s00122-023-04357-3

Cited by 14 publications

(6 citation statements)

References 177 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, genes responsible for meiosis progression and fertilization in model plants have been used for mimicking apomixis in sexual plants, including crops (see details below), and could be targeted in potato. Synthetic apomixis is now being induced in different crops like rice, lettuce ( Lactuca sativa L.), and tomato ( Solanum lycopersicum ), through the combination of multiple mutants using gene-editing technologies [ 34 ]. Yet, a recurrent problem with many of these mutants is that fertility is inevitably compromised and restoring fertility to a level comparable to that of sexual/wild type plants that can be used commercially is an important issue.…”

Section: Breeding Strategies and Challenges To Using Apomixis In Potatomentioning

confidence: 99%

“…While much is known about developmental pathways and reproductive changes, the mechanism underlying the emergence of apomixis is complex and still unknown, but is expected to involve several genes that integrate biological pathways and networks [ 31 ]. Genes underlying apomixis have only recently been found [ 32 , 33 ], but reproductive genes from sexual model plants that generate apomixis-like mutant phenotypes (see Section 5 ) have been known for a while (reviewed in [ 34 ]).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

New Frontiers in Potato Breeding: Tinkering with Reproductive Genes and Apomixis

Hojsgaard,

Nagel,

Feingold

et al. 2024

Biomolecules

View full text Add to dashboard Cite

Potato is the most important non-cereal crop worldwide, and, yet, genetic gains in potato have been traditionally delayed by the crop’s biology, mostly the genetic heterozygosity of autotetraploid cultivars and the intricacies of the reproductive system. Novel site-directed genetic modification techniques provide opportunities for designing climate-smart cultivars, but they also pose new possibilities (and challenges) for breeding potato. As potato species show a remarkable reproductive diversity, and their ovules have a propensity to develop apomixis-like phenotypes, tinkering with reproductive genes in potato is opening new frontiers in potato breeding. Developing diploid varieties instead of tetraploid ones has been proposed as an alternative way to fill the gap in genetic gain, that is being achieved by using gene-edited self-compatible genotypes and inbred lines to exploit hybrid seed technology. In a similar way, modulating the formation of unreduced gametes and synthesizing apomixis in diploid or tetraploid potatoes may help to reinforce the transition to a diploid hybrid crop or enhance introgression schemes and fix highly heterozygous genotypes in tetraploid varieties. In any case, the induction of apomixis-like phenotypes will shorten the time and costs of developing new varieties by allowing the multi-generational propagation through true seeds. In this review, we summarize the current knowledge on potato reproductive phenotypes and underlying genes, discuss the advantages and disadvantages of using potato’s natural variability to modulate reproductive steps during seed formation, and consider strategies to synthesize apomixis. However, before we can fully modulate the reproductive phenotypes, we need to understand the genetic basis of such diversity. Finally, we visualize an active, central role for genebanks in this endeavor by phenotyping properly genotyped genebank accessions and new introductions to provide scientists and breeders with reliable data and resources for developing innovations to exploit market opportunities.

show abstract

Section: Breeding Strategies and Challenges To Using Apomixis In Potatomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

New Frontiers in Potato Breeding: Tinkering with Reproductive Genes and Apomixis

Hojsgaard,

Nagel,

Feingold

et al. 2024

Biomolecules

View full text Add to dashboard Cite

show abstract

“…However, this performance is easily lost due to the random segregation of genetic information in the offspring generations. Apomixis has the potential to allow offspring to retain valuable traits through asexual reproduction, which can lower seed production costs and is important for crop breeding, but apomixis is absent in major crops [122,123]. Thus, how to generate apomixis in crops has become a cutting-edge research hotspot in the field of botany.…”

Section: Promoting Hybrid Rice Asexual Reproductionmentioning

confidence: 99%

CRISPR/Cas Technology Revolutionizes Crop Breeding

Tang,

Wang,

Jin

et al. 2023

Plants

View full text Add to dashboard Cite

Crop breeding is an important global strategy to meet sustainable food demand. CRISPR/Cas is a most promising gene-editing technology for rapid and precise generation of novel germplasm and promoting the development of a series of new breeding techniques, which will certainly lead to the transformation of agricultural innovation. In this review, we summarize recent advances of CRISPR/Cas technology in gene function analyses and the generation of new germplasms with increased yield, improved product quality, and enhanced resistance to biotic and abiotic stress. We highlight their applications and breakthroughs in agriculture, including crop de novo domestication, decoupling the gene pleiotropy tradeoff, crop hybrid seed conventional production, hybrid rice asexual reproduction, and double haploid breeding; the continuous development and application of these technologies will undoubtedly usher in a new era for crop breeding. Moreover, the challenges and development of CRISPR/Cas technology in crops are also discussed.

show abstract

“…Apomictic plants possess the ability to generate seeds genetically identical to the maternal plant [7]. Introducing apomixis into crop plants to perpetuate superior hybrids stands as a goal linked to comprehending the genes and pathways inherent in this process [8].…”

Section: Introductionmentioning

confidence: 99%

Expression Profiling of MSP1 Gene Involved in Mega-gametophyte Development in Ploidy Series of Guinea Grass (Panicum maximum Jacq.)

Choudhary,

Dwivedi,

Dwivedi

et al. 2024

J. Adv. Biol. Biotechnol.

View full text Add to dashboard Cite

Hybrid seeds are playing important role in increasing agricultural production. However, process of hybrid seed development involves high cost for most of the major crops. Under such situation apomixis can play crucial role in creation of one-time hybrid and maintain it for multiple generations. Apomixis and polyploidy are interrelated with each other and expression of apomixis completely relies on polyploidy. Guinea grass (Panicum maximum Jacq.) provides ideal system for research on apomixis and polyploidy because of its extensive diversity in morphological, cytological, biochemical, and molecular characteristics. The experiment was carried out in the eight-ploidy series i.e. 3x, 4x, 5x, 6x, 7x, 8x, 9x, 11x and one obligate sexual Guinea grass genotype SRP75. Considering role of MSP1 gene in apomeiosis, its expression was studied in guinea grass at three developmental stages of spikelet viz., pre-meiotic, meiotic and post-meiotic. Findings of this study highlight the complex regulation of MSP-1 expression during different developmental stages and across various ploidy levels in guinea grass. At pre-meiotic stage, except 4x, downregulation pattern of MSP1 was observed in all the ploidy levels. But opposite was observed in the post-meiotic stage.

show abstract

Engineering apomixis in crops

Cited by 14 publications

References 177 publications

New Frontiers in Potato Breeding: Tinkering with Reproductive Genes and Apomixis

New Frontiers in Potato Breeding: Tinkering with Reproductive Genes and Apomixis

CRISPR/Cas Technology Revolutionizes Crop Breeding

Expression Profiling of MSP1 Gene Involved in Mega-gametophyte Development in Ploidy Series of Guinea Grass (Panicum maximum Jacq.)

Contact Info

Product

Resources

About