Engineering bioluminescent bacteria to boost photodynamic therapy and systemic anti-tumor immunity for synergistic cancer treatment

Yang, Zhijuan; Zhu, Yujie; Dong, Ziliang; Yu, Hao; Wang, Chunjie; Li, Quguang; Wu, Yue; Feng, Liangzhu; Liu, Zhuang

doi:10.1016/j.biomaterials.2021.121332

Cited by 80 publications

(59 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As previously reported, the lysates of tumor and bacterial cells can function as tumor vaccines and initiate potent antitumor immunity 33 , 54 . First, we evaluated the in vivo DCs maturation triggered by Trojan bacteria system.…”

Section: Resultsmentioning

confidence: 69%

“…3f , G422 cells with different treatments are placed in the upper chamber of the transwell system, and DCs collected from the bone marrow of female Balb/c mice about 6–8 weeks old are seeded in the lower chamber. After that, the maturation of DCs (CD11c + , MHC II + ) are evaluated by flow cytometry 53 , 54 . The details of antibody panel for spectral flow cytometry analyses were listed in Supplementary Table 1 .…”

Section: Resultsmentioning

confidence: 99%

“…Meanwhile, it was shown that pure bacteria (EC or VNP) treatment could also prime similar antitumor immunity, but mostly lower than those elicited by the Trojan bacteria-induced photothermal treatment. We concluded that the Trojan bacteria under laser irradiation achieved the thermolysis of GBM cells, promoting the release of tumor-associated antigens (TAAs) 33 , 54 , 61 . Meanwhile, Trojan bacteria under laser irradiation could kill the host bacterial cells to promote the release of diverse pathogen-associated molecular patterns (PAMPs), which could promote the activation of innate immune cells, such as macrophages and NK cells 33 , 54 , 60 , 62 .…”

Section: Resultsmentioning

confidence: 99%

“…We concluded that the Trojan bacteria under laser irradiation achieved the thermolysis of GBM cells, promoting the release of tumor-associated antigens (TAAs) 33 , 54 , 61 . Meanwhile, Trojan bacteria under laser irradiation could kill the host bacterial cells to promote the release of diverse pathogen-associated molecular patterns (PAMPs), which could promote the activation of innate immune cells, such as macrophages and NK cells 33 , 54 , 60 , 62 . On the other aspect, these PAMPs would elicit potent adaptive antitumor immune response by promoting tumor-infiltrating frequencies of activated CD8 + T cells.…”

Section: Resultsmentioning

confidence: 99%

See 3 more Smart Citations

Bacteria loaded with glucose polymer and photosensitive ICG silicon-nanoparticles for glioblastoma photothermal immunotherapy

Sun

Liu

et al. 2022

Nat Commun

View full text Add to dashboard Cite

Bacteria can bypass the blood-brain barrier (BBB), suggesting the possibility of employment of bacteria for combating central nervous system diseases. Herein, we develop a bacteria-based drug delivery system for glioblastoma (GBM) photothermal immunotherapy. The system, which we name as ‘Trojan bacteria’, consists of bacteria loaded with glucose polymer and photosensitive ICG silicon-nanoparticles. In an orthotopic GBM mouse model, we demonstrate that the intravenously injected bacteria bypass the BBB, targeting and penetrating GBM tissues. Upon 808 nm-laser irradiation, the photothermal effects produced by ICG allow the destruction of bacterial cells and the adjacent tumour cells. Furthermore, the bacterial debris as well as the tumour-associated antigens promote antitumor immune responses that prolong the survival of GBM-bearing mice. Moreover, we demonstrate the residual bacteria are effectively eliminated from the body, supporting the potential therapeutic use of this system.

show abstract

Section: Resultsmentioning

confidence: 69%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Bacteria loaded with glucose polymer and photosensitive ICG silicon-nanoparticles for glioblastoma photothermal immunotherapy

Sun

Liu

et al. 2022

Nat Commun

View full text Add to dashboard Cite

show abstract

“…During the past few decades, PDT, as a noninvasive strategy for treating esophageal tumors, skin tumors, and lung tumors in the clinic with light, photosensitizer (PS) and oxygen, has been regarded as an extremely promising for high-efficient cancer therapeutic modality ( Yang et al, 2022 ). However, the efficacies of PDT are far from satisfactory because PS poorly accumulates and can hardly penetrate tumor sites.…”

Section: Applications Of Bacteria-mediated Strategies In Cancer Therapymentioning

confidence: 99%

Recent advances in bacteria-mediated cancer therapy

Liang

Wang

Shao

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Cancer is among the leading cause of deaths worldwide. Although conventional therapies have been applied in the fight against the cancer, the poor oxygen, low extracellular pH, and high interstitial fluid pressure of the tumor microenvironment mean that these treatments fail to completely eradicate cancer cells. Recently, bacteria have increasingly been considered to be a promising platform for cancer therapy thanks to their many unique properties, such as specific tumor-targeting ability, high motility, immunogenicity, and their use as gene or drug carriers. Several types of bacteria have already been used for solid and metastatic tumor therapies, with promising results. With the development of synthetic biology, engineered bacteria have been endowed with the controllable expression of therapeutic proteins. Meanwhile, nanomaterials have been widely used to modify bacteria for targeted drug delivery, photothermal therapy, magnetothermal therapy, and photodynamic therapy, while promoting the antitumor efficiency of synergistic cancer therapies. This review will provide a brief introduction to the foundation of bacterial biotherapy. We begin by summarizing the recent advances in the use of many different types of bacteria in multiple targeted tumor therapies. We will then discuss the future prospects of bacteria-mediated cancer therapies.

show abstract

Self‐Promoted Targeting Delivery of Nanodrug Through Chemotherapeutic Upregulation of CD47 for Triple Negative Breast Cancer Therapy

Liu,

Chen,

Jiang

et al. 2023

Adv Funct Materials

View full text Add to dashboard Cite

The high heterogeneity of receptor expression and varied chemotherapeutic sensitivity seriously compromise the therapeutic outcome of triple‐negative breast cancer (TNBC)‐targeting nanodrugs. In this work, a TNBC‐targeting nanodrug (designated as CPU) equipped with self‐promoted targeting property through chemotherapeutic upregulation of CD47 is constructed, enabling synergistic chemo/photodynamic therapy. Specifically, the hydrophobic docetaxel (DOC), which can upregulate the expression of CD47 in TNBC cells, is loaded in the nanomicelles (designated as P‐Pep) assembled from a protoporphyrin IX (PPIX)‐labeled amphiphilic chimeric peptide of Fmoc‐K(PPIX)‐AWSATWSNYWRH, obtaining the ca. 196 nm CPU. Aided by the enhanced permeability and retention effect and guidance of CD47‐binding peptide sequence, CPU is verified to prefer to accumulate in CD47 overexpressed TNBC cells, which can recruit more CPU by upregulating CD47 expression post‐treatment of DOC. Both in vitro and in vivo results demonstrate the superior tumor targeting ability, which can extensively amplify chemotherapeutic and photodynamic therapeutic effects while evading obvious adverse effects. The self‐promoted targeting strategy will inspire the design of nanodrugs for the personalized therapy of tumors with high heterogeneity and resistance.

show abstract

Engineering bioluminescent bacteria to boost photodynamic therapy and systemic anti-tumor immunity for synergistic cancer treatment

Cited by 80 publications

References 43 publications

Bacteria loaded with glucose polymer and photosensitive ICG silicon-nanoparticles for glioblastoma photothermal immunotherapy

Bacteria loaded with glucose polymer and photosensitive ICG silicon-nanoparticles for glioblastoma photothermal immunotherapy

Recent advances in bacteria-mediated cancer therapy

Self‐Promoted Targeting Delivery of Nanodrug Through Chemotherapeutic Upregulation of CD47 for Triple Negative Breast Cancer Therapy

Contact Info

Product

Resources

About