Engineering biomolecular systems: Controlling the self-assembly of gelatin to form ultra-small bioactive nanomaterials

Suresh, Dhananjay; Suresh, Agasthya; Kannan, Raghuraman

doi:10.1016/j.bioactmat.2022.02.035

Cited by 15 publications

(9 citation statements)

References 77 publications

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“…Peaks also appear at the respective positions in the measured surface potentials. We may only speculate about the nature of these anomalies but they may be caused by interlinked molecules clumping at the surface [54,77,78] , for which they might be avoidable by an optimized adsorption procedure. In the experiment, the peaks were evaded by selecting a spot at which the separation a could be reduced to below 50 nm without snap to contact.…”

Section: B Experimental Resultsmentioning

confidence: 99%

Efficient Reduction of Casimir Forces by Self-assembled Bio-molecular Thin Films

Sedmik

Urech

Zalevsky

et al. 2023

Preprint

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Casimir forces, related to London-van der Waals forces, arise if the spectrum of electromagnetic fluctuations is restricted by boundaries. There is great interest both from fundamental science and technical applications to control these forces on the nano scale. Scientifically, the Casimir effect being the only known quantum vacuum effect manifesting between macroscopic objects, allows to investigate the poorly known physics of the vacuum. In this work, we experimentally investigate the influence of self-assembled molecular bio and organic thin films on the Casimir force between a plate and a sphere. We find that molecular thin films, despite being a mere few nanometers thick, reduce the Casimir force by up to 14%. To identify the molecular characteristics leading to this reduction, five different bio-molecular films with varying chemical and physical properties were investigated. Spectroscopic data reveal a broad absorption band whose presence can be attributed to the mixing of electronic states of the underlying gold layer and those of the molecular film due to charge rearrangement in the process of self-assembly. Using Lifshitz theory we calculate that the observed change in the Casimir force is consistent with the appearance of the new absorption band due to the formation of molecular layers. The desired Casimir force reduction can be tuned by stacking several monolayers, using a simple self-assembly technique in a solution. The molecules - each a few nanometers long - can penetrate small cavities and holes, and cover any surface with high efficiency. This process seems compatible with current methods in the production of micro-electromechanical systems (MEMS), which cannot be miniaturized beyond a certain size due to `stiction' caused by the Casimir effect. Our approach could therefore readily enable further miniaturization of these devices.

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Section: B Experimental Resultsmentioning

confidence: 99%

Efficient Reduction of Casimir Forces by Self-assembled Bio-molecular Thin Films

Sedmik

Urech

Zalevsky

et al. 2023

Preprint

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“…Suresh et al self-assembled gelatin chains using anionic, phosphate-based cross-linkers to product ultrasmall GNPs (G x ) with a size of 10 nm. 119 The "�NH 2 " groups on the main chain of collagen were protonated and linked using ionic cross-linkers. Small ionic cross-linkers could selectively facilitate the connection of ammonium ions within the same chain rather than between different chains.…”

Section: Nanoprecipitationmentioning

confidence: 99%

Chemical and Structural Engineering of Gelatin-Based Delivery Systems for Therapeutic Applications: A Review

Jia,

Fan,

Chen

et al. 2024

Biomacromolecules

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As a biodegradable and biocompatible protein derived from collagen, gelatin has been extensively exploited as a fundamental component of biological scaffolds and drug delivery systems for precise medicine. The easily engineered gelatin holds great promise in formulating various delivery systems to protect and enhance the efficacy of drugs for improving the safety and effectiveness of numerous pharmaceuticals. The remarkable biocompatibility and adjustable mechanical properties of gelatin permit the construction of active 3D scaffolds to accelerate the regeneration of injured tissues and organs. In this Review, we delve into diverse strategies for fabricating and functionalizing gelatin-based structures, which are applicable to gene and drug delivery as well as tissue engineering. We emphasized the advantages of various gelatin derivatives, including methacryloyl gelatin, polyethylene glycolmodified gelatin, thiolated gelatin, and alendronate-modified gelatin. These derivatives exhibit excellent physicochemical and biological properties, allowing the fabrication of tailor-made structures for biomedical applications. Additionally, we explored the latest developments in the modulation of their physicochemical properties by combining additive materials and manufacturing platforms, outlining the design of multifunctional gelatin-based micro-, nano-, and macrostructures. While discussing the current limitations, we also addressed the challenges that need to be overcome for clinical translation, including high manufacturing costs, limited application scenarios, and potential immunogenicity. This Review provides insight into how the structural and chemical engineering of gelatin can be leveraged to pave the way for significant advancements in biomedical applications and the improvement of patient outcomes.

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“…This information will allow readers to fully evaluate the correct choice of parameters based on the particles/solvent system being analyzed. 39 The selection of accumulation time could be done manually or automatically when the software determines the time required to collect a good quality correlation curve. If an experimentalist sets the accumulation time in a manual mode, we suggest that the number of collected counts during the experiment should be close to 1 000 000.…”

Section: Bad Quality Dls Experiments and Misinterpretation Of The Rep...mentioning

confidence: 99%

Dynamic light scattering and transmission electron microscopy in drug delivery: a roadmap for correct characterization of nanoparticles and interpretation of results

Filippov,

Khusnutdinov,

Murmiliuk

et al. 2023

Mater. Horiz.

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Engineering biomolecular systems: Controlling the self-assembly of gelatin to form ultra-small bioactive nanomaterials

Cited by 15 publications

References 77 publications

Efficient Reduction of Casimir Forces by Self-assembled Bio-molecular Thin Films

Efficient Reduction of Casimir Forces by Self-assembled Bio-molecular Thin Films

Chemical and Structural Engineering of Gelatin-Based Delivery Systems for Therapeutic Applications: A Review

Dynamic light scattering and transmission electron microscopy in drug delivery: a roadmap for correct characterization of nanoparticles and interpretation of results

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