Engineering Cell Surfaces via Liposome Fusion

Dutta, Dipak Kumar; Pulsipher, Abigail; Luo, Wei; Mak, Hugo; Yousaf, Muhammad N.

doi:10.1021/bc200236m

Cited by 72 publications

(88 citation statements)

References 47 publications

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“…1). The interfacial oxime reaction is fast, chemoselective, occurs at physiological conditions (37 °C, pH 7) and requires no catalyst272829. Furthermore, the resulting oxime bond has no side reactions with biomacromolecules, is bio-orthogonal and therefore does not interfere with native biological processes30.…”

Section: Resultsmentioning

confidence: 99%

Scaffold Free Bio-orthogonal Assembly of 3-Dimensional Cardiac Tissue via Cell Surface Engineering

Rogozhnikov

O’Brien

Elahipanah

et al. 2016

Sci Rep

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There has been tremendous interest in constructing in vitro cardiac tissue for a range of fundamental studies of cardiac development and disease and as a commercial system to evaluate therapeutic drug discovery prioritization and toxicity. Although there has been progress towards studying 2-dimensional cardiac function in vitro, there remain challenging obstacles to generate rapid and efficient scaffold-free 3-dimensional multiple cell type co-culture cardiac tissue models. Herein, we develop a programmed rapid self-assembly strategy to induce specific and stable cell-cell contacts among multiple cell types found in heart tissue to generate 3D tissues through cell-surface engineering based on liposome delivery and fusion to display bio-orthogonal functional groups from cell membranes. We generate, for the first time, a scaffold free and stable self assembled 3 cell line co-culture 3D cardiac tissue model by assembling cardiomyocytes, endothelial cells and cardiac fibroblast cells via a rapid inter-cell click ligation process. We compare and analyze the function of the 3D cardiac tissue chips with 2D co-culture monolayers by assessing cardiac specific markers, electromechanical cell coupling, beating rates and evaluating drug toxicity.

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Section: Resultsmentioning

confidence: 99%

Scaffold Free Bio-orthogonal Assembly of 3-Dimensional Cardiac Tissue via Cell Surface Engineering

Rogozhnikov

O’Brien

Elahipanah

et al. 2016

Sci Rep

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“…Cell surface tailoring was achieved by a straightforward new liposome fusion method to incorporate complementary bio-orthogonal molecules capable of an intercellular click chemical reaction upon physical cell-cell contact1314. The external cell surface click conjugation between cells proceeds at physiological conditions in the presence of serum and allows for stable cell interconnectivity.…”

Section: Resultsmentioning

confidence: 99%

Remote Control of Tissue Interactions via Engineered Photo-switchable Cell Surfaces

Luo

Pulsipher

Dutta

et al. 2014

Sci Rep

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We report a general cell surface molecular engineering strategy via liposome fusion delivery to create a dual photo-active and bio-orthogonal cell surface for remote controlled spatial and temporal manipulation of microtissue assembly and disassembly. Cell surface tailoring of chemoselective functional groups was achieved by a liposome fusion delivery method and quantified by flow cytometry and characterized by a new cell surface lipid pull down mass spectrometry strategy. Dynamic co-culture spheroid tissue assembly in solution and co-culture tissue multilayer assembly on materials was demonstrated by an intercellular photo-oxime ligation that could be remotely cleaved and disassembled on demand. Spatial and temporal control of microtissue structures containing multiple cell types was demonstrated by the generation of patterned multilayers for controlling stem cell differentiation. Remote control of cell interactions via cell surface engineering that allows for real-time manipulation of tissue dynamics may provide tools with the scope to answer fundamental questions of cell communication and initiate new biotechnologies ranging from imaging probes to drug delivery vehicles to regenerative medicine, inexpensive bioreactor technology and tissue engineering therapies.

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“…Dutta and colleagues demonstrated this strategy on fibroblasts. Various functional groups or fluorescent dyes can be transferred from the liposomes onto cells [86]. A similar approach has been applied to MSC for surface modification.…”

Section: Techniques For Engineering Mscmentioning

confidence: 99%

Engineering mesenchymal stem cells for regenerative medicine and drug delivery

Park

Suryaprakash

Lao

et al. 2015

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Researchers have applied mesenchymal stem cells (MSC) to a variety of therapeutic scenarios by harnessing their multipotent, regenerative, and immunosuppressive properties with tropisms toward inflamed, hypoxic, and cancerous sites. Although MSC-based therapies have been shown to be safe and effective to a certain degree, the efficacy remains low in most cases when MSC are applied alone. To enhance their therapeutic efficacy, researchers have equipped MSC with targeted delivery functions using genetic engineering, therapeutic agent incorporation, and cell surface modification. MSC can be genetically modified virally or non-virally to overexpress therapeutic proteins that complement their innate properties. MSC can also be primed with non-peptidic drugs or magnetic nanoparticles for enhanced efficacy and externally regulated targeting, respectively. Furthermore, MSC can be functionalized with targeting moieties to augment their homing toward therapeutic sites using enzymatic modification, chemical conjugation, or non-covalent interactions. These engineering techniques are still works in progress, requiring optimization to improve the therapeutic efficacy and targeting effectiveness while minimizing any loss of MSC function. In this review, we will highlight the advanced techniques of engineering MSC, describe their promise and the challenges of translation into clinical settings, and suggest future perspectives on realizing their full potential for MSC-based therapy.

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Engineering Cell Surfaces via Liposome Fusion

Cited by 72 publications

References 47 publications

Scaffold Free Bio-orthogonal Assembly of 3-Dimensional Cardiac Tissue via Cell Surface Engineering

Scaffold Free Bio-orthogonal Assembly of 3-Dimensional Cardiac Tissue via Cell Surface Engineering

Remote Control of Tissue Interactions via Engineered Photo-switchable Cell Surfaces

Engineering mesenchymal stem cells for regenerative medicine and drug delivery

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