Engineering dynamic biointerfaces

Andrews, Ross N.; Co, Carlos C.; Ho, Chia‐Chi

doi:10.1016/j.coche.2015.11.005

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…Dynamic control of cell behavior on material surfaces is of great importance for both fundamental research in cell biology and practical applications such as tissue engineering, drug delivery, and cell-based diagnostics. − In recent years, increasing efforts have been made to construct so-called “smart” biointerfaces with switchable functions to regulate cell–surface interactions in response to changes in external stimuli. − Among the available stimuli, light is particularly attractive due to its noninvasive and intrinsically clean nature, making it suitable to be used in biological systems without risk of compromising normal biological function. − A widely used strategy to construct photoresponsive biointerfaces is to attach photoswitchable molecules onto solid supports. For example, azobenzene (Azo) is a photoresponsive molecule that could reversibly form inclusion complexes with host molecules such as cyclodextrin (CD) in response to light of different wavelengths. , The rodlike trans Azo forms a stable complex with CD, whereas the “bent” cis Azo does not fit in the CD cavity due to size mismatch. − Several dynamic supramolecular platforms based on Azo/CD have been developed for remote control of biointerfacial interactions such as capture and release of mammalian cells , and bacteria …”

Section: Introductionmentioning

confidence: 99%

Smart Biointerface with Photoswitched Functions between Bactericidal Activity and Bacteria-Releasing Ability

Wei

Zhan

et al. 2017

ACS Appl. Mater. Interfaces

133

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Smart biointerfaces with capability to regulate cell-surface interactions in response to external stimuli are of great interest for both fundamental research and practical applications. Smart surfaces with "ON/OFF" switchability for a single function such as cell attachment/detachment are well-known and useful, but the ability to switch between two different functions may be seen as the next level of "smart". In this work reported, a smart supramolecular surface capable of switching functions reversibly between bactericidal activity and bacteria-releasing ability in response to UV-visible light is developed. This platform is composed of surface-containing azobenzene (Azo) groups and a biocidal β-cyclodextrin derivative conjugated with seven quaternary ammonium salt groups (CD-QAS). The surface-immobilized Azo groups in trans form can specially incorporate CD-QAS to achieve a strongly bactericidal surface that kill more than 90% attached bacteria. On irradiation with UV light, the Azo groups switch to cis form, resulting in the dissociation of the Azo/CD-QAS inclusion complex and release of dead bacteria from the surface. After the kill-and-release cycle, the surface can be easily regenerated for reuse by irradiation with visible light and reincorporation of fresh CD-QAS. The use of supramolecular chemistry represents a promising approach to the realization of smart, multifunctional surfaces, and has the potential to be applied to diverse materials and devices in the biomedical field.

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Section: Introductionmentioning

confidence: 99%

Smart Biointerface with Photoswitched Functions between Bactericidal Activity and Bacteria-Releasing Ability

Wei

Zhan

et al. 2017

ACS Appl. Mater. Interfaces

133

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“…Alternatively, the chemical change can be triggered by activating the substrate, thus allowing the immobilization of ligands present in solution. In some cases, the reaction can even be reversed, leading to the release of the ligand from the substrate. − These strategies rely on interfacial chemical reactions initiated by an external noninvasive stimulus, such as light or an electrical potential, ,, enabling a spatiotemporal control over the surface environment. , Dynamic control over the properties of surfaces can be performed by applying an electrical potential to generate oxidation or reduction reactions to attached molecules, leading to two different redox states. For example, a switch in surface wettability could be obtained by altering the electronic states of single-layered molecules between hydrophilic and hydrophobic states, yielding interesting substrates for microfluidic, self-cleaning, or sensor devices .…”

Section: Introductionmentioning

confidence: 99%

Stimuli-Responsive Functionalization Strategies to Spatially and Temporally Control Surface Properties: Michael vs Diels–Alder Type Additions

et al. 2018

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Stimuli-responsive self-assembled monolayers (SAMs) are used to confer switchable physical, chemical, or biological properties to surfaces through the application of external stimuli. To obtain spatially and temporally tunable surfaces, we present microcontact printed SAMs of a hydroquinone molecule that are used as a dynamic interface to immobilize different functional molecules either via Diels-Alder or Michael thiol addition reactions upon the application of a low potential. In spite of the use of such reactions and the potential applicability of the resulting surfaces in different fields ranging from sensing to biomedicine through data storage or cleanup, a direct comparison of the two functionalization strategies on a surface has not yet been performed. Although the Michael thiol addition requires molecules that are commercial or easy to synthesize in comparison with the cyclopentadiene derivatives needed for the Diels-Alder reaction, the latter reaction produces more homogeneous coverages under similar experimental conditions.

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Stimuli‐Responsive Neuronal Networking via Removable Alginate Masks

2018

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The neuron patterning method has been applied to cultured neuronal networks to achieve better reproducibility and various experimental conditions in brain research. However, as models of dynamic environments, conventional patterned neural networks having an unalterable structure formed by a preprogrammed substrate have limitations, such as replicating the changes in the physical structure of networks in vivo. This study presents a novel culture technique that can control the location of the neuronal cell bodies constituting the network and the connection of the isolated neuronal networks at a desired time point. Alginate hydrogel, which has a cell‐repellent property and is dissolvable by weak chemical treatment, is used as a background masking material for patterning. Axon growth from neural clusters to the background area formed by a microwell‐patterned alginate layer is possible due to removal of the alginate layer, triggering synchronized spontaneous firing of the neuronal clusters. This technique was combined with a microelectrode array system to observe dynamic changes in neuronal activity according to the transformed morphology of the network. Finally, the clustered network structure formed by this technique showed greatly improved spontaneous firing rates and spike amplitudes compared to the network model prepared using an existing uniform structure.

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Engineering dynamic biointerfaces

Cited by 4 publications

References 39 publications

Smart Biointerface with Photoswitched Functions between Bactericidal Activity and Bacteria-Releasing Ability

Smart Biointerface with Photoswitched Functions between Bactericidal Activity and Bacteria-Releasing Ability

Stimuli-Responsive Functionalization Strategies to Spatially and Temporally Control Surface Properties: Michael vs Diels–Alder Type Additions

Stimuli‐Responsive Neuronal Networking via Removable Alginate Masks

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