Engineering herbicide-resistant watermelon variety through CRISPR/Cas9-mediated base-editing

Tian, Shouwei; Jiang, Linjian; Cui, Xiaxia; Zhang, Jie; Guo, Sheng; Li, Maoying; Zhang, Haiying; Ren, Yi; Gong, Guoyi; Zong, Ming; Liu, Fan; Chen, Qijun; Xu, Yong

doi:10.1007/s00299-018-2299-0

Cited by 246 publications

(137 citation statements)

References 10 publications

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“…In another application, dCas9 can be fused to base editing enzymes such as cytidine deaminase to induce specific missense mutations at the targeted genomic region (Figure C). A successful application was reported in creating herbicide‐resistant watermelon as described earlier (Tian et al ). In addition, dCas9 can be fused to chromatin modifying enzymes including DNA methytransferase, histone deacetylase, and others to epigenetically modulate transcription at specific genomic locus (Xu et al ) (Figure D).…”

Section: Discussionmentioning

confidence: 94%

“…Watermelon ( Citrullus lanatus) is the second species in the Cucurbitaceae family with successful genome editing (Tian et al ; ). By first targeting ClPDS gene with standard CRISPR/Cas9, almost all the transgenic watermelon plants harbor ClPDS mutations, indicating almost 100% editing efficiency.…”

Section: Overview Of Fruit Crop Genome Editingmentioning

confidence: 99%

“…However, some T 1 progeny plants not only became homozygous for the edited mutation, but also lost the dCas9 transgene. These transgene‐free homozygous als mutant plants were shown to be resistant to the herbicide Tribenuron (Tian et al ). This is a very exciting development using the dCas9‐base editing platform.…”

Section: Overview Of Fruit Crop Genome Editingmentioning

confidence: 99%

“…With the sgRNA providing targetspecificity, the dCas9-base editors can create point mutations in the target DNA with high specificity and precision. This new technology was quickly adopted in plants, including watermelon (Lu and Zhu 2017;Li et al 2018a;Ren et al 2018;Tian et al 2018). Table 1 summarizes published reports of genome editing in fruit crops.…”

Section: Overview Of Crispr/cas9mentioning

confidence: 99%

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Application and future perspective of CRISPR/Cas9 genome editing in fruit crops

Zhou¹,

Wang

et al. 2019

JIPB

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Fruit crops, including apple, orange, grape, banana, strawberry, watermelon, kiwifruit and tomato, not only provide essential nutrients for human life but also contribute to the major agricultural output and economic growth of many countries and regions in the world. Recent advancements in genome editing provides an unprecedented opportunity for the genetic improvement of these agronomically important fruit crops. Here, we summarize recent reports of applying CRISPR/Cas9 to fruit crops, including efforts to reduce disease susceptibility, change plant architecture or flower morphology, improve fruit quality traits, and increase fruit yield. We discuss challenges facing fruit crops as well as new improvements and platforms that could be used to facilitate genome editing in fruit crops, including dCas9‐base‐editing to introduce desirable alleles and heat treatment to increase editing efficiency. In addition, we highlight what we see as potentially revolutionary development ranging from transgene‐free genome editing to de novo domestication of wild relatives. Without doubt, we now see only the beginning of what will eventually be possible with the use of the CRISPR/Cas9 toolkit. Efforts to communicate with the public and an emphasis on the manipulation of consumer‐friendly traits will be critical to facilitate public acceptance of genetically engineered fruits with this new technology.

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Section: Discussionmentioning

confidence: 94%

Section: Overview Of Fruit Crop Genome Editingmentioning

confidence: 99%

Section: Overview Of Fruit Crop Genome Editingmentioning

confidence: 99%

Section: Overview Of Crispr/cas9mentioning

confidence: 99%

See 2 more Smart Citations

Application and future perspective of CRISPR/Cas9 genome editing in fruit crops

Zhou¹,

Wang

et al. 2019

JIPB

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“…Watermelon (Citrullus lanatus var. lanatus) dominates the fruit market in the world [3], its growth frequently restricted by high temperatures reach more than 35℃ during summer months [4]. Bottle gourd (Lagenaria siceraria (Mol.)…”

Section: Introductionmentioning

confidence: 99%

QTL-Seq Identifies QTL for Relative Electrical Conductivity Associated with Heat Tolerance in Bottle Gourd (Lagenaria Siceraria)

Song

Huang

2019

Preprint

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Heat is a major abiotic stressor seriously affects watermelon produce; however, its effects may be mitigated through grafting onto heat tolerant bottle gourd rootstock. To understand the genetic basis and select reliable markers are necessary in the variety breeding process. In this paper, the relative electric conductivity (REC) was used as a visual indicator for heat stress to study the genetics and SNP marker of heat tolerance in bottle gourd via QTL-seq approach. The results showed that recessive inheritance and a major QTL locus controlled REC related to heat tolerance. Seven heat-tolerant loci (qHT1. 1, qHT2.1, qHT2.2, qHT5.1, qHT6.1, qHT7.1, and qHT8.1) exhibited high Δ(SNP-index) values (ranging from 0.19-0.38), and of these, the greatest value (0.32, P < 0.01) and greatest number of detected SNPs (9052) were found in chromosome 2 at qHT2.1 region (11.03 -19.25 Mb); qHT2.1was taken as a promising major QTL for heat tolerance in bottle gourd. A total of 34 putative candidate genes related to heat stress were detected within the qHT2.1 region. Three polymorphic SNPs (BG_GLEAN_10022642, BG_GLEAN_10022727, and BG_GLEAN_10022589) that are involved in pollen sterility, intracellular transport, and signal recognition were validated and exhibited significant marker-trait association that could be used in heat tolerant molecular breeding for bottle gourd. The qHT2.1region is an important finding can be used for fine mapping and discovering new genes associated with heat tolerance in bottle gourd.

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