“…Therefore each case of anaerobic digestion should be pretested, to determine the feasibility of adding the crude glycerol. Factors such as pH, temperature, redox potential, organic loading rate (OLR), hydraulic retention time (HRT), macronutrients, micronutrients, trace elements (TE), volatile fatty acids (VFA), ammonia, and electrical conductivity (EC) play an important role in the growth and activity of microorganisms (Roussel et al, 2019;Wyman et al, 2019). Recent studies have shown that microelements such as iron (Fe), zinc (Zn), chromium (Cr), copper (Cu), magnesium (Mg), nickel (Ni), manganese (Mn), barium (Ba), and others play an important role in the biogas production process (Schattauer et al, 2011;Wyman et al, 2019).…”