Engineering Rubisco to enhance CO2 utilization

Table_1.DOCX

“…Rubisco can also catalyze the addition of O 2 to ribulose 1,5-bisphosphate, resulting in production of 2-phosphoglycoate, a process called photorespiration, which reduces carbon fixation rates. It has been widely accepted that the CBB cycle in photosynthetic organism is bottlenecked by the slow reaction kinetics of rubisco which fixes only 1-22 CO 2 molecules per second per active site (Zhao et al, 2024), in part due to the requirement for specificity of CO 2 over O 2 binding. However, carboxylation rates are faster in cyanobacterial species, which is likely linked to the lower requirement for CO 2 specificity, as rubisco is enclosed in the CO 2 enriched carboxysome (Savir et al, 2010).…”