Engineering the Extracellular Environment: Strategies for Building 2D and 3D Cellular Structures

Guillame-Gentil, Orane; Semenov, Oleg V.; Roca, Ana Sala; Groth, Thomas; Zahn, Raphael; Vörös, János; Zenobi‐Wong, Marcy

doi:10.1002/adma.201001747

Cited by 159 publications

(141 citation statements)

References 252 publications

(183 reference statements)

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“…hormones, cytokines, growth factors) to be presented to cells, and iii) possess tuned biodegradability. [2][3] The issues above are challenging for the fabrication of artificial ECM but are essential for successful applications in tissue engineering, diagnostics, and animal-free studies.…”

mentioning

confidence: 99%

Biodegradation-Resistant Multilayers Coated with Gold Nanoparticles. Toward a Tailor-made Artificial Extracellular Matrix

Prokopović

Vikulina

Sustr

et al. 2016

ACS Appl. Mater. Interfaces

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Abstract:Polymer multicomponent coatings such as multilayers mimic extracellular matrix (ECM) that attracts significant attention for their use as functional supports for advanced cell culture and tissue engineering. Herein, biodegradation and molecular transport in hyaluronan/polylysine multilayers coated with gold nanoparticles was described. Nanoparticle 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 2 coating acts as semipermeable barrier that governs molecular transport into/from the multilayers and makes them biodegradation resistant. Model protein lysozyme (mimics of ECM soluble signals) diffuses in the multilayers as fast and slow diffusing populations existing in an equilibrium. Such composite system may have high potential to be exploited as degradationresistant drug delivery platforms suitable for cell-based applications.The extracellular matrix (ECM) provides not only a structural support for cellular growth, but also a biochemical milieu, which together define cell fate. 1 Artificial ECM is designed to mimic real ECM and to achieve cellular functions in laboratory designed materials similar as in nature.Artificial ECM consists of degradable supports made of natural or (semi)synthetic polymers which: i) recapitulate physical-chemical properties of real ECM, ii) host bioactive signaling molecules (e.g. hormones, cytokines, growth factors) to be presented to cells, and iii) possess tuned biodegradability. 2-3 The issues above are challenging for the fabrication of artificial ECM but are essential for successful applications in tissue engineering, diagnostics, and animal-free studies.Introduced in the early nineties, 4 layer-by-layer deposition proofed to be extremely useful in the field of bio-functional coatings. It is powerful technique for the fabrication of polymer-based multilayer films with dimensions from nano to micro, adjusted composition, and tunable cellular response. [5][6][7][8][9][10][11] Control over cellular functions such as adhesion, differentiation, and proliferation, 12-15 along with reservoir properties for a variety of biomolecules such as proteins, growth factors, genes, and drugs 7, 16-20 makes these films an attractive platform for cell-based applications.Despite the evident biological potential to employ the multilayers to recapitulate ECM, 3 the options to tune multilayer biodegradability are mostly limited to multilayer chemical 13,[21][22] that can significantly change multilayer properties. There is also a lack in understanding the mechanism of biomolecule-multilayer interaction that obviously defines biomolecule presentation/release from multilayers to cells and, as a result, cellular response. [23][24] In this work a new approach for the design of artificial ECM with controlled biodegradation based on the coating of multilayers with gold nanoparticles (GNPs) is present. The multilayers wer...

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mentioning

confidence: 99%

Biodegradation-Resistant Multilayers Coated with Gold Nanoparticles. Toward a Tailor-made Artificial Extracellular Matrix

Prokopović

Vikulina

Sustr

et al. 2016

ACS Appl. Mater. Interfaces

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“…The deposition cycles were repeated until the desired number of bilayers had been prepared. In this study (HA/PLL) 24 films have been produced. The films were stored in TRIS buffer at 4 1C.…”

Section: Preparation Of (Ha/pll) 24 Filmsmentioning

confidence: 99%

“…The ''refreshment'' allows the minimization of the possible reorganization effects during film storage. 24 films with microgels (HA/PLL) 24 films were exposed to 1 mL of 2 mg mL À1 microgel solution in TRIS buffer for 1 h followed by extensive washing with the buffer.…”

Section: Preparation Of (Ha/pll) 24 Filmsmentioning

confidence: 99%

“…A mixture of PLL-FITC with PLL (1 : 150 molar ratio) with a final polymer concentration of 0.5 mg mL À1 in TRIS buffer is added to the (HA/PLL) 24 -MG film deposited onto cover glass. The fluorescence increase in the film has been measured using a fluorescent microscope (Cell-R, Olympus).…”

Section: Pll Diffusion Into the (Ha/pll) 24 Filmmentioning

confidence: 99%

“…22,23 Extensive reviews on the reservoir properties of the multilayers can be found elsewhere. 6,7,10,24 Multilayers assembled from the biopolymers hyaluronic acid (HA) and poly-L-lysine (PLL) are some of the most studied systems. The HA/PLL multilayers are non-toxic for a variety of cell lines and have been employed to control cell adhesion via chemical or physical cues.…”

Section: Introductionmentioning

confidence: 99%

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Temperature-induced molecular transport through polymer multilayers coated with PNIPAM microgels

Vikulina

Aleed

Paulraj

et al. 2015

Phys. Chem. Chem. Phys.

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Polyelectrolyte multilayers serve as effective reservoirs for bioactive molecules which are stored and released from the multilayers for cellular applications. However, control over the release without significantly affecting the multilayers and biomolecules is still a challenge. On the other hand, externally stimulated release would make the multilayers promising for the development of stimuli-sensitive planar carriers with release performance switched on demand. In this study soft composite films are designed by coating hyaluronic acid/poly-L-lysine (HA/PLL) multilayers with temperature responsive poly-(N-isopropylacrylamide) (PNIPAM) microgels. Microgels are flattened and immersed into the multilayers to maximize the number of contacts with the surrounding polyelectrolytes (HA and PLL). The microgel coating serves as an efficient switchable barrier for the PLL transport into the multilayers. PLL diffusion into the film is significantly hindered at room temperature but is dramatically enhanced at 40 1C above the volume phase transition temperature (VPTT) of PNIPAM at 32 1C associated with microgel shrinkage. Scanning force microscopy micrographs show that the mechanism of volume phase transition on soft surfaces cannot be directly deduced from the processes taking place at solid substrates.

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Self‐Assembled Monolayers as Model Biosurfaces

Laromaine¹,

Mace²

2013

Organic Nanomaterials

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Engineering the Extracellular Environment: Strategies for Building 2D and 3D Cellular Structures

Cited by 159 publications

References 252 publications

Biodegradation-Resistant Multilayers Coated with Gold Nanoparticles. Toward a Tailor-made Artificial Extracellular Matrix

Biodegradation-Resistant Multilayers Coated with Gold Nanoparticles. Toward a Tailor-made Artificial Extracellular Matrix

Temperature-induced molecular transport through polymer multilayers coated with PNIPAM microgels

Self‐Assembled Monolayers as Model Biosurfaces

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