2003
DOI: 10.1038/nbt850
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Engineering tolerance and accumulation of lead and cadmium in transgenic plants

Abstract: We have studied the utility of the yeast protein YCF1, which detoxifies cadmium by transporting it into vacuoles, for the remediation of lead and cadmium contamination. We found that the yeast YCF1-deletion mutant DTY167 was hypersensitive to Pb(II) as compared with wild-type yeast. DTY167 cells overexpressing YCF1 were more resistant to Pb(II) and Cd(II) than were wild-type cells, and accumulated more lead and cadmium. Analysis of transgenic Arabidopsis thaliana plants overexpressing YCF1 showed that YCF1 is … Show more

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Cited by 369 publications
(174 citation statements)
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“…Vacuoles were prepared from Arabidopsis mesophyll protoplasts as described previously (44,45). Transport studies with Arabidopsis mesophyll vacuoles were performed as described for barley (44,45) but using silicone oil poly(dimethylsiloxane-co-methylphenylsiloxane) 550 (Aldrich) instead of AR200.…”
Section: Isolation Of Intact Vacuoles From Arabidopsis Protoplasts Anmentioning
confidence: 99%
See 1 more Smart Citation
“…Vacuoles were prepared from Arabidopsis mesophyll protoplasts as described previously (44,45). Transport studies with Arabidopsis mesophyll vacuoles were performed as described for barley (44,45) but using silicone oil poly(dimethylsiloxane-co-methylphenylsiloxane) 550 (Aldrich) instead of AR200.…”
Section: Isolation Of Intact Vacuoles From Arabidopsis Protoplasts Anmentioning
confidence: 99%
“…Transport studies with Arabidopsis mesophyll vacuoles were performed as described for barley (44,45) but using silicone oil poly(dimethylsiloxane-co-methylphenylsiloxane) 550 (Aldrich) instead of AR200. The assay for apoPC 2 Transport rates were calculated by subtracting the radioactivity taken up after 20 min from that after 2.5 min incubation.…”
Section: Isolation Of Intact Vacuoles From Arabidopsis Protoplasts Anmentioning
confidence: 99%
“…The preparation of intact Arabidopsis mesophyll vacuoles was based on previously described procedures (Frangne et al, 2002;Song et al, 2003), which were further optimized. All experimental steps were performed on ice, and all centrifugations were carried out without break, unless otherwise stated.…”
Section: Isolation Of Arabidopsis Mesophyll Vacuolesmentioning
confidence: 99%
“…A suggested means to overcome this limitation, making phytoremediation a feasible technology, is to transfer the genes responsible for the tolerance and hyperaccumulation of heavy metals to fast-growing, high-biomass plants (Meagher, 2000). Although progress has been made towards the achievement of this goal, and transgenic plants with enhanced tolerance to and accumulation of cadmium and other heavy metals have been produced (Zhu et al, 1999;Domínguez-Solis et al, 2001;Gong et al, 2003;Song et al, 2003), our molecular knowledge about metal hyperaccumulation remains limited, and investigation is still needed to understand the genetic mechanisms responsible for heavy metal accumulation and detoxification. Genes belonging to different functional categories have been characterized in relation to their role in cadmium tolerance and accumulation.…”
Section: Introductionmentioning
confidence: 99%