Engraftment for CD34 selected stem cell products is not compromised by cryopreservation

Abstract

Cryopreservation of a CD34+-selected product can be safely used in a combined transplant with UCB and does not affect engraftment time.

Cryopreservation of adult unrelated donor products in hematopoietic cell transplantation: the OneMatch experience and systematic review of the literature

“…A subset of studies provided data on hematopoietic engraftment and the impact of cryopreservation on cell yield in comparison to controls. No apparent difference in time to platelet (PLT) recovery (10 studies reporting median days to PLT count of >20 3 10 9 /L; 14,17,18,21,28,[30][31][32][33][34] five studies reporting median days to PLT count of >50 3 10 9 /L 7,19,26,37,38 ) or neutrophil engraftment (18 studies reporting median days to neutrophil count of >0.5 3 10 9 / Rescheduling issue 3 Fig. 1.…”

“…L 7,14,15,[17][18][19]21,[26][27][28][30][31][32][33][34][36][37][38] ) was reported in any of the studies in comparison to controls (Table 5). Moreover, no apparent difference in CD341 cells infused (eight studies 9,14,[17][18][19]21,29,31 ) was reported for patients receiving cryopreserved products compared to controls receiving fresh products ( Table 5). The yield of CD341 cells available after thawing was 31% to 100% (data reported in eight studies 9,13,21,24,26,[35][36][37] ).…”

“…The yield of CD341 cells available after thawing was 31% to 100% (data reported in eight studies 9,13,21,24,26,[35][36][37] ). Only 13 studies 12,13,[16][17][18][19][20]26,30,32,35,36,38 (40% of studies, reporting on 575 patients or 57.9% of total) reported reasons for performing cryopreservation. While most studies reported more than one reason for performing cryopreservation, few specified the precise number of products for each cited reason.…”

“…Number of days to ANC of >0.5 3 10 9 /L (n 5 18 studies) 9-21. 12,13,[16][17][18][19][20]26,30,32,35,36,38 Reason for cryopreservation request was possible. The impact of cryopreservation on hematopoietic engraftment appears negligible when compared to controls although more systematic reporting in studies that includes mean values for rates of engraftment and yields of viable total cells and CD341 cells will be needed to increase confidence and allow for pooled metaanalysis.…”

Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model

“…In an article published in 1970, Mazur described various factors that can cause injury to cells during cryopreservation [ 28 ]. Even though cryopreservation has been studied in detail over the last four decades, there are conflicting results leading to positive and negative consequences [ 27 , 29 , 30 ]. In the present study, we evaluated the effects of cryopreservation on the cellular viability, composition, and phenotype of the various subpopulations of mice bone marrow mononuclear cells (BM-MNCs).…”