Enhance Growth and Biochemical Composition of Nannochloropsis oceanica, Cultured under Nutrient Limitation, Using Commercial Agricultural Fertilizers

Abstract

Microalgae culture media should be economic, allow for high growth, satisfy the needs of microalgal cells and easy to prepare. In this study, we evaluate the effect of different media formula prepared from commercial agricultural fertilizers (CAGF), comparing to F/2 Guillard standard medium as a control medium, on growth (cell density, CD; dry weight, DW and specific growth rate, µ) and biochemical composition (lipid, protein, and carbohydrate) of Nannochloropsis oceanica. Comparing to N/P ratio (9.6) and actually quantity (12.36 g/l and 1.29 g/l, respectively) of F/2 standard medium, six N/P ratios (19.2, 9.6, 9.6, 4.8, 3.2 and 1.6) were prepared from Nitric Acid (N-Nt) or Ammonium Sulphate (N-Am), as a nitrogen source, with phosphoric acid (P), as a phosphorus source, for culturing media of N. oceanica. The results investigated that some CAGF media achieved significant (P ≤ 0.05) growth and biochemical composition higher than F/2. Comparing to lipid percentage (30.70 %) of F/2, the lipid percentage of N. oceanica cultured on different CAGF media were ranging from 18.40% to 46.12%, depending on nutrient limitation, nitrogen source, N/P ratios and actually atom concentrations. Finally, the use of CAGF constitutes a viable alternative of F/2 medium to reduce the production costs N. oceanica, the commonly used in marine hatcheries and also other biotechnological applications.

Potential Applications of Native Cyanobacterium Isolate (Arthrospira platensis NIOF17/003) for Biodiesel Production and Utilization of Its Byproduct in Marine Rotifer (Brachionus plicatilis) Production

“…Matta et al [72] cited that the lipid content of A. platensis ranging from 4 to 16%. However, the differences between our results and those obtained by other studies, even those isolated from the same site of El-Khadra Lake, may be due to different culture systems, scales, extraction methods, environmental conditions [21,36,37,[68][69][70][71][72][73], or available nutrient limitation [73].…”

“…The aquatic organisms are a sustainable source of PUFA, MUFA, SFAs, palmitic acid, and fatty acid methyl esters [74,75]. Among all aquatic organisms, microalgal cells are the most sustainable, attractive, and promising source [1][2][3][17][18][19][20]69,72,73]. Cohen [76] stated that about 50 percent of the total lipid of Arthrospira is fatty acids.…”

Effect of Pterocladia capillacea Seaweed Extracts on Growth Parameters and Biochemical Constituents of Jew’s Mallow

“…Marine algae are considered very important bioindicator for the marine ecosystem [13][14][15]. Many studies have reported that the constituents, diversity, and communities of marine algae are affected by variations in environmental parameters and nutrient limitation [65][66][67][68][69][70]. During the last few years, the attention on scientific and commercial interest to biotechnological applications of algae as a sustainable source and global commercial for aquaculture [71][72][73][74][75], biofuel [12,76], extracts [77,78], food supplement, pharmaceuticals, and cosmetics were increased [12].…”

“…However, our data is in the same line of the results presented by Khairy and El-Shafay [13] who found that, during spring season of 2010, the highest component is carbohydrate (50.49%), followed by protein (23.72%) and lipid (2.71%). Many authors reported that the biochemical constituents of marine algae are affected by variations in environmental conditions and nutrient availability [65][66][67][68][69][70].…”