Enhanced accumulation of individual ganoderic acids in a submerged culture of Ganoderma lucidum by the overexpression of squalene synthase gene

Zhou, Jinhua; Ji, Senlin; Ren, Meng-Fei; He, Yilong; Jing, Xiaoran; Xu, Junwei

doi:10.1016/j.bej.2014.06.008

Cited by 57 publications

(45 citation statements)

References 29 publications

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“…This has been demonstrated as a promising approach for the production of various compounds including polysaccharides (Fang and Zhong, 2002, Wagner et al., 2003, Habijanic et al., 2015). Genetically engineering strains to increase the production of ganodermic acids in submerged cultures has also enjoyed some success (Zhou et al., 2014, Xu et al., 2015, Li et al., 2016). As well as being a faster approach, the use of submerged cultures allows improved control over various fermentation conditions such as nutrient availability, pH and oxygen, which allows optimisation and greater reproducibility.…”

Section: Medicinal Basidiomycetesmentioning

confidence: 99%

The good, the bad and the tasty: The many roles of mushrooms

Mattos-Shipley¹,

Ford²,

Alberti³

et al. 2016

Studies in Mycology

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Fungi are often inconspicuous in nature and this means it is all too easy to overlook their importance. Often referred to as the “Forgotten Kingdom”, fungi are key components of life on this planet. The phylum Basidiomycota, considered to contain the most complex and evolutionarily advanced members of this Kingdom, includes some of the most iconic fungal species such as the gilled mushrooms, puffballs and bracket fungi. Basidiomycetes inhabit a wide range of ecological niches, carrying out vital ecosystem roles, particularly in carbon cycling and as symbiotic partners with a range of other organisms. Specifically in the context of human use, the basidiomycetes are a highly valuable food source and are increasingly medicinally important. In this review, seven main categories, or ‘roles’, for basidiomycetes have been suggested by the authors: as model species, edible species, toxic species, medicinal basidiomycetes, symbionts, decomposers and pathogens, and two species have been chosen as representatives of each category. Although this is in no way an exhaustive discussion of the importance of basidiomycetes, this review aims to give a broad overview of the importance of these organisms, exploring the various ways they can be exploited to the benefit of human society.

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Section: Medicinal Basidiomycetesmentioning

confidence: 99%

The good, the bad and the tasty: The many roles of mushrooms

Mattos-Shipley¹,

Ford²,

Alberti³

et al. 2016

Studies in Mycology

View full text Add to dashboard Cite

show abstract

“…Genetic transformation systems, including Agrobacterium tumefacien ‐mediated and polyethylene glycol (PEG)‐mediated transformations, have been developed in G. lucidum (Shi et al , ; Xu et al , ; Xu and Zhong, ) and have been successfully used to enhance the production of bioactive compounds, such as ganoderic acids and polysaccharides (Zhou et al , ; Li et al , ; Zhang et al , , ; Fei et al , ; Xu et al , ). Gene silencing systems have also been used for the partial suppression of genes of interest in G. lucidum (Mu et al , ; Liu et al , ; Zhu et al , ).…”

Section: Introductionmentioning

confidence: 99%

Dual sgRNA‐directed gene deletion in basidiomycete Ganoderma lucidum using the CRISPR/Cas9 system

Liu

Sun

You

et al. 2020

Microbial Biotechnology

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Ganoderma lucidum is an important medicinal mushroom in traditional Chinese medicine. However, the lack of adequate genetic tools has hindered molecular genetic research in and the genetic modification of this species. Here, we report that the presence of an intron is necessary for the efficient expression of the heterologous phosphinothricin‐resistance and green fluorescent protein genes in G. lucidum. Moreover, we improved the CRISPR/Cas9‐mediated gene disruption frequency in G. lucidum by adding an intron upstream of the Cas9 gene. Our results showed that the disruption frequency of the orotidine 5’‐monophosphate decarboxylase gene (ura3) in transformants containing the glyceraldehyde‐3‐phosphate dehydrogenase gene intron in the Cas9 plasmid is 14–18 in 107 protoplasts, which is 10.6 times higher than that in transformants without any intron sequence. Furthermore, genomic fragment deletions in the ura3 and GL17624 genes were achieved via a dual sgRNA‐directed CRISPR/Cas9 system in G. lucidum. We achieved a ura3 deletion frequency of 36.7% in G. lucidum. The developed method provides a powerful platform to generate gene deletion mutants and will facilitate functional genomic studies in G. lucidum.

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“…In 2014, the triterpenoid biosynthesis pathway in A. cinnamomea was validated through genomic and transcriptome analyses; this pathway is similar to the MVA pathway in Ganoderma lucidum [13]. Several enzymes, such as squalene synthase (SQS) [14], HMG-CoA reductase (HMGR) [15], mevalonate pyrophosphate decarboxylase (MVD) [16], and farnesyldiphosphate synthase (FPS) [17], are implicated in the MVA pathway. A previous study showed that the synthesis of triterpenoids in A. cinnamomea is complex.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and expression analysis of mevalonate pyrophosphate decarboxylase inAntrodia cinnamomea

Lin

Shao

et al. 2016

MATEC Web of Conferences

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Abstract. Mevalonate pyrophosphate decarboxylase (MVD) plays important roles in triterpenoid biosynthesis via the mevalonate pathway. A novel MVD gene was isolated and identified in Antrodia cinnamomea (Ac-mvd). The fulllength cDNA contained an open reading frame with a length of 1,209 bp and encoded a 402-amino acid polypeptide with a molecular mass of 43.3 kDa and a theoretical pI of 8.23. As incubation time was prolonged for 28 days, the triterpenoid content in the mycelium gradually increased and reached 39.192 ± 2.025 mg/g; the triterpenoid content of the fruiting body grew in the hay of Cinnamomum kanehirae (ACFB-CK), was 49.391 ± 2.675 mg/g, which was significantly different from other samples (P < 0.05). qRT-PCR revealed that the highest expression levels of Ac-mvd in the mycelium of Antrodia cinnamomea were detected on the 7th day. The expression levels gradually decreased as culture time was extended from 14 days to 42 days.

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Enhanced accumulation of individual ganoderic acids in a submerged culture of Ganoderma lucidum by the overexpression of squalene synthase gene

Cited by 57 publications

References 29 publications

The good, the bad and the tasty: The many roles of mushrooms

The good, the bad and the tasty: The many roles of mushrooms

Dual sgRNA‐directed gene deletion in basidiomycete Ganoderma lucidum using the CRISPR/Cas9 system

Molecular cloning and expression analysis of mevalonate pyrophosphate decarboxylase inAntrodia cinnamomea

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