Enhanced alcohol yields in batch cultures of Clostridium acetobutylicum using a three-electrode potentiometric system with methyl viologen as electron carrier

Peguin, S.; Delorme, P.; Goma, G.; Soucaille, Philippe

doi:10.1007/bf00134624

Cited by 43 publications

(24 citation statements)

References 16 publications

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“…Reductions in acid production and yield were observed. These results coincide with previously reported findings (Peguin et al, 1994). The effect of using different methyl viologen addition times was also studied and is discussed later.…”

Section: Ab Fermentation Under Various Conditionssupporting

confidence: 93%

“…This can be achieved by decreasing the agitation rate , increasing the H 2 partial pressure (Mollah and Stuckey, 1992;, or CO gassing (Datta and Zeikus, 1985;Meyer et al, 1986). In this study, an electron carrier, methyl viologen, was used to alter the electron flow (Kim and Kim, 1988;Peguin et al, 1994;Mutharasan, 1986, 1987). Methyl viologen was chosen because it satisfies a number of requirements as a good electron mediator (Allen and Bowen, 1985), and it can replace FdH 2 in all reactions in which this protein is involved.…”

Section: Ab Fermentation Under Various Conditionsmentioning

confidence: 99%

See 1 more Smart Citation

Metabolism analysis and on-line physiological state diagnosis of acetone–butanol fermentation

Chauvatcharin

Siripatana

Seki

et al. 1998

Biotechnol. Bioeng.

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Section: Ab Fermentation Under Various Conditionssupporting

confidence: 93%

Section: Ab Fermentation Under Various Conditionsmentioning

confidence: 99%

Metabolism analysis and on-line physiological state diagnosis of acetone–butanol fermentation

Chauvatcharin

Siripatana

Seki

et al. 1998

Biotechnol. Bioeng.

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“…To our knowledge, the purely electrochemical reduction of organics to ethanol has not been described in literature. Bioelectrochemical reductions of organics to ethanol, however, have been described (Peguin et al 1994;Shin et al 2002;Steinbusch et al 2009). Recently, Steinbusch et al (2009) found that acetate could be reduced to ethanol by mixed cultures in presence of a redox mediator such as methyl viologen.…”

Section: Ethanolmentioning

confidence: 99%

New applications and performance of bioelectrochemical systems

Hamelers

Heijne

Sleutels

et al. 2009

Appl Microbiol Biotechnol

260

134

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Bioelectrochemical systems (BESs) are emerging technologies which use microorganisms to catalyze the reactions at the anode and/or cathode. BES research is advancing rapidly, and a whole range of applications using different electron donors and acceptors has already been developed. In this mini review, we focus on technological aspects of the expanding application of BESs. We will analyze the anode and cathode half-reactions in terms of their standard and actual potential and report the overpotentials of these half-reactions by comparing the reported potentials with their theoretical potentials. When combining anodes with cathodes in a BES, new bottlenecks and opportunities arise. For application of BESs, it is crucial to lower the internal energy losses and increase productivity at the same time. Membranes are a crucial element to obtain high efficiencies and pure products but increase the internal resistance of BESs. The comparison between production of fuels and chemicals in BESs and in present production processes should gain more attention in future BES research. By making this comparison, it will become clear if the scope of BESs can and should be further developed into the field of biorefineries.

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“…Microbial electrolysis cells (MECs) have been used to produce gaseous electrofuels such as hydrogen and methane (9,32,38). Other bioelectrochemical systems (BESs) have produced ethanol and butanol (28,34). There is a growing interest in using such technologies to produce longer-chain hydrocarbon fuels and other higher-value products (23,28,30).…”

mentioning

confidence: 99%

“…Other bioelectrochemical systems (BESs) have produced ethanol and butanol (28,34). There is a growing interest in using such technologies to produce longer-chain hydrocarbon fuels and other higher-value products (23,28,30).…”

mentioning

confidence: 99%

Enrichment of Microbial Electrolysis Cell Biocathodes from Sediment Microbial Fuel Cell Bioanodes

Pisciotta

Zaybak

Call

et al. 2012

Appl Environ Microbiol

174

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Electron-accepting (electrotrophic) biocathodes were produced by first enriching graphite fiber brush electrodes as the anodes in sediment-type microbial fuel cells (sMFCs) using two different marine sediments and then electrically inverting the anodes to function as cathodes in two-chamber bioelectrochemical systems (BESs). Electron consumption occurred at set potentials of ؊439 mV and ؊539 mV (versus the potential of a standard hydrogen electrode) but not at ؊339 mV in minimal media lacking organic sources of energy. Results at these different potentials were consistent with separate linear sweep voltammetry (LSV) scans that indicated enhanced activity (current consumption) below only ca. ؊400 mV. MFC bioanodes not originally acclimated at a set potential produced electron-accepting (electrotrophic) biocathodes, but bioanodes operated at a set potential (؉11 mV) did not. CO 2 was removed from cathode headspace, indicating that the electrotrophic biocathodes were autotrophic. Hydrogen gas generation, followed by loss of hydrogen gas and methane production in one sample, suggested hydrogenotrophic methanogenesis. There was abundant microbial growth in the biocathode chamber, as evidenced by an increase in turbidity and the presence of microorganisms on the cathode surface. Clone library analysis of 16S rRNA genes indicated prominent sequences most similar to those of Eubacterium limosum (Butyribacterium methylotrophicum), Desulfovibrio sp. A2, Rhodococcus opacus, and Gemmata obscuriglobus. Transfer of the suspension to sterile cathodes made of graphite plates, carbon rods, or carbon brushes in new BESs resulted in enhanced current after 4 days, demonstrating growth by these microbial communities on a variety of cathode substrates. This report provides a simple and effective method for enriching autotrophic electrotrophs by the use of sMFCs without the need for set potentials, followed by the use of potentials more negative than ؊400 mV.

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Enhanced alcohol yields in batch cultures of Clostridium acetobutylicum using a three-electrode potentiometric system with methyl viologen as electron carrier

Cited by 43 publications

References 16 publications

Metabolism analysis and on-line physiological state diagnosis of acetone–butanol fermentation

Metabolism analysis and on-line physiological state diagnosis of acetone–butanol fermentation

New applications and performance of bioelectrochemical systems

Enrichment of Microbial Electrolysis Cell Biocathodes from Sediment Microbial Fuel Cell Bioanodes

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