Enhanced antibacterial and anticancer properties of Se-NPs decorated TiO2 nanotube film

Abstract

Selenium nanoparticle modified surfaces attract increasing attention in the field of tissue engineering. Selenium exhibits strong anticancer, antibacterial and anti-inflammatory properties and it maintains relatively low off-target cytotoxicity. In our paper, we present the fabrication, characterization and cytocompatibility of titanium oxide (TiO2) nanotube surface decorated with various surface densities of chemically synthesized selenium nanoparticles. To evaluate antibacterial and anti-cancer properties of such nanostructured surface, gram negative bacteria E. coli, cancerous osteoblast like MG-63 cells and non-cancerous fibroblast NIH/3T3 were cultured on designed surfaces. Our results suggested that selenium nanoparticles improved antibacterial properties of titanium dioxide nanotubes and confirmed the anticancer activity towards MG-63 cells, with increasing surface density of nanoparticles. Further, the selenium decorated TiO2 nanotubes suggested deteriorating effect on the cell adhesion and viability of non-cancerous NIH/3T3 cells. Thus, we demonstrated that selenium nanoparticles decorated TiO2 nanotubes synthesized using sodium selenite and glutathione can be used to control bacterial infections and prevent the growth of cancerous cells. However, the higher surface density of nanoparticles adsorbed on the surface was found to be cytotoxic for non-cancerous NIH/3T3 cells and thus it might complicate the integration of biomaterial into the host tissue. Therefore, an optimal surface density of selenium nanoparticles must be found to effectively kill bacteria and cancer cells, while remaining favorable for normal cells.

In vitro evaluation of bone cements impregnated with selenium nanoparticles stabilized by phosphatidylcholine (PC) for application in bone

“…Similarly, as depicted by Perni et al, when the totality of the paraben is released from HA, but only about 5% of the initial amount of propylparaben is released from PMMA. 44 Furthermore, the percentage of the released selenium proportionally increased with increasing surface density of nanoparticles as in research done by Bilek et al 45 For instance, after 4 days, the SeNPs release from 15PC-SeNPs-TCP began to improve by 97.46 ppb when compared to (Figure 9), the numbers of viable E. coli bacteria on the surfaces of the TCP, especially on 15TCP, significantly reduced with respect to PMMA. The bacterial count for PMMA was found to be 34.2 CFU.10 5 mL À1 , when for 15PC-SeNPs-TCP was 0.2 CFU.10 5 mL À1 (p < 0.005).…”

“…44 Furthermore, the percentage of the released selenium proportionally increased with increasing surface density of nanoparticles as in research done by Bilek et al. 45 For instance, after 4 days, the SeNPs release from 15PC-SeNPs-TCP began to improve by 97.46 ppb when compared to 25.82 ppb from 2.5PC-SeNPs-TCP. Similarly, released selenium at the end of 3 days was 18.8 ppb for 2.5PC-SeNPs-PMMA when 121 ppb for 15PC-SeNPs-PMMA.…”

Selenium Nanoparticles as Candidates for Antibacterial Substitutes and Supplements against Multidrug-Resistant Bacteria

“…Among them, selenium nanoparticles (SeNPs) have attracted increased attention. Selenium (Se) is an essential trace element for humans (about 50 µg/day), and antimicrobial studies using different forms of Se, such as selenite and SeNPs, have been reported [28][29][30][31][32][33]. Se is a significant element, because it is an important cofactor for antioxidant enzymes such as glutathione peroxidase and thioredoxin reductase [34,35].…”

Study of 1D Titanate‐Based Materials –New Modification of the Synthesis Procedure and Surface Properties‐Recent Applications

“…TiNTs have potential applications in several fields; they are found in biomaterials, gas sensors, adsorbents, heterogeneous catalysis ( photocatalysis, oxidation reactions, organic synthesis, biomass,…), biological activities to remove bacteria from water, solar cells, Li battery production, and many others. In particular, TiNTs have been used as a catalytic support for different active species (metals, oxides and sulfides).…”