Enhanced bioactivity of bone morphogenetic protein-2 with low dose of 2-N, 6-O-sulfated chitosan in vitro and in vivo

Expression of bone morphogenetic protein-2 after using chitosan gel with different molecular weight on wound healing process of dental extraction

“…6,12 Chitosan is the partially deacetylated form poly-(1,4)-2-amino-2-deoxy-D-glucose) of chitin, and is a potential biomaterial for bone tissue engineering application. 1,10,12 In the present study, the chitosan powder was mixed with blood of each person and filled in dental socket, and it found that bone tissue regeneration will be faster in chitosan-filled socket than untreated dental socket. 13 In our study it was used chitosan gel material with different molecular weight to analyzed expressions of BMP-2 Rattus norvegicus on wound healing process of dental extraction.…”

“…It plays critical roles in osteogenesis and bone metabolism. 1 The bone matrix is rich in growth factors, among which bone morphogenetic proteins (BMP s ), that are synthesized and secreted by osteoblast and incorporated into the matrix during bone formation. The BMP s , released during osteoclastic bone resorption, are capable of inducing differentiation of mesenchymal cells into osteoblasts (osteinduction), stimulating bone formation in both remodeling and repairing process.…”

“…The BMP s , released during osteoclastic bone resorption, are capable of inducing differentiation of mesenchymal cells into osteoblasts (osteinduction), stimulating bone formation in both remodeling and repairing process. 1,2 Chitosan is carbohydrate copolymer composed of glucosamine and N-acetyl-D-glucosamine units. Chitosan has a number of properties including biocompatibility, biodegradability, mucoadhesiveness and wound healing capabilities that make it useful as a biomaterial.…”

Lack of effect of bone morphogenetic protein 2 and 4 gene polymorphisms on bone density in postmenopausal Turkish women

“…BMPs may modulate the action of IGF-I via the type 1 IGF receptor and/or IGF binding proteins (Takahashi et al, 2007). 2-N, 6-O-sulfated chitosan (26SCS) is a more potent enhancer of BMP-2 bioactivity and induces osteoblastic differentiation in vitro and in vivo by promoting the BMP-2 signaling pathway, suggesting that 26SCS could be used as the synergistic factor of BMP-2 for bone regeneration (Zhou et al, 2009). It has been shown that the fibrillin-microfibril network is essential in the extracellular control of BMP signaling (Sengle et al, 2008).…”

In vivomodulatory effects of chitooligosaccharide nanoparticles on mouse serum cytokines and splenocytes

“…2-N,6-O-SC was prepared according to Zhou et al [17]. In brief, 50 mL of formamide (MW 45.04 Da; Wako, Osaka, Japan) and 2 mL formic acid (MW 46.03 Da; Wako, Osaka, Japan) was added to 500 mL three-necked bottomed flask containing 0.5 g chitosan.…”