Enhanced Cadaverine Production by Engineered Escherichia coli Using Soybean Residue Hydrolysate (SRH) as a Sole Nitrogen Source

Guo, Xianmin; Li, Mengyang; Li, Hui; Xu, Sheng; He, Xun; Ouyang, Pingkai; Chen, Kequan

doi:10.1007/s12010-020-03444-1

Cited by 5 publications

(3 citation statements)

References 27 publications

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“…Whole-cell synthesis of cadaveric amines was performed in a 1 L fermenter (Infors, Switzerland) with a conversion fluid volume of 0.3 L. The transformation broth contained 50 g/L L-lysine and 3 g DCW/L of engineered Escherichia coli DFC1001 ( Guo et al, 2020 ) with or without EPSs. Approximately 100–300 mg of EPSs were added per gram of DCW E. coli DFC1001.…”

Section: Methodsmentioning

confidence: 99%

“…Biotransformation was performed at 37°C, 300 rpm, and the pH was adjusted to 7.0 by the automatic addition of 2 M HCl. After 1 h of biotransformation, the surviving bacteria in the culture solution were calculated by plating on Luria-Bertani (LB) agar plates containing 50 μg/mL streptomycin ( Guo et al, 2020 ), followed by serial dilution. The survival rate of engineered Escherichia coli DFC1001 was defined as the percentage of viable cells after and before whole-cell synthesis.…”

Section: Methodsmentioning

confidence: 99%

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Screening of exopolysaccharide-producing Enterobacter aerogenes NJ1023 and its cadaverine biosynthesis promotion

Xie¹,

Ye²,

Wan³

et al. 2023

Front. Microbiol.

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Enterobacter aerogenes, the gram-negative bacteria belonging to the family Enterobacteriaceae, lacks the ability to synthesize chemicals. However, in this study, a strain of Enterobacter aerogenes NJ1023 screened from the soil containing petrochemicals was found to be capable of producing extracellular polysaccharides (EPSs). After purification of the polysaccharide, the chemical composition and physicochemical properties of the polysaccharide were analyzed by UV–Vis spectra, FTIR spectroscopy and GC-MS, etc. The results showed that: The molecular weight of the polysaccharide produced by this strain was only 2.7×103 Da, which was lower than that reported in other polysaccharides from the same genus. The polysaccharide produced by E. aerogenes NJ1023 mainly comprised xylose, glucose, galactose, and N-acetylglucosamine with a molar ratio of 0.27: 4.52: 1.74: 0.2, which differed from those reported from the same genus. The results demonstrated that lower incubation temperatures and shaking speeds were more favorable for EPSs synthesis, while higher incubation temperatures and shaking speeds favored cell growth. Additionally, the EPSs produced by E. aerogenes NJ1023 significantly protected the Escherichia coli cells against cadaverine stress. Overall, the discovery of EPSs produced by E. aerogenes increased the diversity of bacterial polysaccharides and broadened the potential applications of this species.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Screening of exopolysaccharide-producing Enterobacter aerogenes NJ1023 and its cadaverine biosynthesis promotion

Xie¹,

Ye²,

Wan³

et al. 2023

Front. Microbiol.

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“…To produce bio-based polyamides from sustainable resources, C. glutamicum was engineered to produce cadaverine from starch ( Tateno et al, 2007 , 2009 ) and from xylose ( Buschke et al, 2013 ). Also, other model organisms like E. coli have been successfully engineered, and cadaverine could be produced from soybean hydrolysates ( Guo et al, 2021 ). Aside from cadaverine, ω-amino acids GABA and 5AVA were produced in C. glutamicum from empty fruit bunch sugars ( Baritugo et al, 2018 ) and from a Miscanthus and a rice straw hydrolysate ( Joo et al, 2017 ; Sasikumar et al, 2021 ), respectively.…”

Section: Introductionmentioning

confidence: 99%

Utilization of a Wheat Sidestream for 5-Aminovalerate Production in Corynebacterium glutamicum

Burgardt

Prell

Wendisch

2021

Front. Bioeng. Biotechnol.

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Production of plastics from petroleum-based raw materials extensively contributes to global pollution and CO2 emissions. Biotechnological production of functionalized monomers can reduce the environmental impact, in particular when using industrial sidestreams as feedstocks. Corynebacterium glutamicum, which is used in the million-ton-scale amino acid production, has been engineered for sustainable production of polyamide monomers. In this study, wheat sidestream concentrate (WSC) from industrial starch production was utilized for production of l-lysine–derived bifunctional monomers using metabolically engineered C. glutamicum strains. Growth of C. glutamicum on WSC was observed and could be improved by hydrolysis of WSC. By heterologous expression of the genes xylAXcBCg (xylA from Xanthomonas campestris) and araBADEc from E. coli, xylose, and arabinose in WSC hydrolysate (WSCH), in addition to glucose, could be consumed, and production of l-lysine could be increased. WSCH-based production of cadaverine and 5-aminovalerate (5AVA) was enabled. To this end, the lysine decarboxylase gene ldcCEc from E. coli was expressed alone or for conversion to 5AVA cascaded either with putrescine transaminase and dehydrogenase genes patDAEc from E. coli or with putrescine oxidase gene puoRq from Rhodococcus qingshengii and patDEc. Deletion of the l-glutamate dehydrogenase–encoding gene gdh reduced formation of l-glutamate as a side product for strains with either of the cascades. Since the former cascade (ldcCEc-patDAEc) yields l-glutamate, 5AVA production is coupled to growth by flux enforcement resulting in the highest 5AVA titer obtained with WSCH-based media.

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Microbial Production of Amines and Amino Acids by Fermentation

Wendisch

Kerbs

2022

Microbial Production of High-Value Products

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Enhanced Cadaverine Production by Engineered Escherichia coli Using Soybean Residue Hydrolysate (SRH) as a Sole Nitrogen Source

Cited by 5 publications

References 27 publications

Screening of exopolysaccharide-producing Enterobacter aerogenes NJ1023 and its cadaverine biosynthesis promotion

Screening of exopolysaccharide-producing Enterobacter aerogenes NJ1023 and its cadaverine biosynthesis promotion

Utilization of a Wheat Sidestream for 5-Aminovalerate Production in Corynebacterium glutamicum

Microbial Production of Amines and Amino Acids by Fermentation

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