Enhanced cardioprotective effects by coexpression of two isoforms of hepatocyte growth factor from naked plasmid DNA in a rat ischemic heart disease model

Abstract

These results demonstrate that transfer of the genomic-cDNA hybrid expressing both isoforms of the HGF gene might provide higher therapeutic effects than the cDNA sequence producing HGF(728) alone in the treatment of ischemic heart disease.

Phase 1/2 Open-label Dose-escalation Study of Plasmid DNA Expressing Two Isoforms of Hepatocyte Growth Factor in Patients With Painful Diabetic Peripheral Neuropathy

“…The safety and efficacy of VM202 for cardiovascular diseases have been demonstrated previously in extensive preclinical studies [7][8][9][10][11][12][13] as well as three independent phase I trials. [14][15][16] VM202 is a plasmid DNA containing a therapeutic gene, HGF-X7, which encodes two isoforms of hepatocyte growth factor (HGF).…”

“…[14][15][16] VM202 is a plasmid DNA containing a therapeutic gene, HGF-X7, which encodes two isoforms of hepatocyte growth factor (HGF). 7 HGF is a multifunctional, mesenchymederived cytokine with potent angiogenic and antifibrotic activities. [17][18][19][20][21][22] To maximize its therapeutic effects, this plasmid DNA was designed to simultaneously express two naturally occurring isoforms of HGF, HGF 723 and HGF 728 , which consist of 723 and 728 amino acids, respectively.…”

The cytokines of pulmonary fibrosis: Much learned, much more to learn

“…The relevance of HGF to pulmonary fibrosis has been emphasized by the findings of impaired activation of pro-HGF in patients with IPF [214] and in the bleomycin model in mice [215]. There have been substantial developments in exploring HGF and its engineered derivatives as promising therapeutic agents for fibrosis [212,216,217].…”

Assessment of Genetic Mutations DMD, DYSF, EMD, LMNA, DUX4, DMPK, ZNF9, PABPN1 Genes Induction Duchenne Muscular Dystrophy

“…The resulting mixture for 24 hours at -20°C was incubated. [21][22][23][24][25][26][27] 2. Then for 45min at 4°C and was centrifuged at 12000 rpm era.…”