Enhanced cell viability in hyaluronic acid coated poly(lactic-co-glycolic acid) porous scaffolds within microfluidic channels

Zamboni, Fernanda; Keays, Marie; Hayes, Sheri L.; Albadarin, Ahmad B.; Walker, Gavin; Kiely, Patrick A.; Collins, Maurice N.

doi:10.1016/j.ijpharm.2017.09.053

Cited by 69 publications

(33 citation statements)

References 43 publications

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“…In the case of HA, the use of human keratinocytes (La Gatta et al, 2016 ), HaCaT (Sacco et al, 2016 ; Sun et al, 2017 ); murine cell lines [NIH-3T3 (Sigen et al, 2018 ), or L929 (Zamboni et al, 2017 )] have been reported. It is advisable to employ cell type homologous with the tissue/organ concerns for the specific application, i.e., endothelial cells for testing stents (Choi et al, 2016 ; Hauser et al, 2017 ), human dermal fibroblasts and epidermal keratinocytes for wound healing (D'Agostino et al, 2015 ), adipose-derived stem cells for dermal fillers or gels (Guo et al, 2017 ; Stellavato et al, 2017 ) and chondrocytes for cartilage knee repair (Brittberg, 2014 ).…”

Section: Biological Testing and Safety Assessmentmentioning

confidence: 99%

An Effective Translation: The Development of Hyaluronan-Based Medical Products From the Physicochemical, and Preclinical Aspects

Huerta-Ángeles

Nešporová

Ambrožová

et al. 2018

Front. Bioeng. Biotechnol.

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This review shows the steps toward material selection focalized on the design and development of medical devices based on hyaluronan (HA). The selection is based on chemical and mechanical properties, biocompatibility, sterilization, safety, and scale-up costs. These facts play a vital role in the industrialization process. Approved medical devices containing-HA are illustrated to identify key parameters. The first part of this work involves the steps toward a complete characterization of chemical and mechanical aspects, reproducibility of the processes and scale up. In a second stage, we aimed to describe the preclinical in vitro and in vivo assays and selected examples of clinical trials. Furthermore, it is important to keep in mind the regulatory affairs during the research and development (R&D) using standardization (ISO standards) to achieve the main goal, which is the functionality and safety of the final device. To keep reproducible experimental data to prepare an efficient master file for the device, based on quality and recorded manufacturing data, and a rigorous R&D process may help toward clinical translation. A strong debate is still going on because the denominated basic research in HA field does not pay attention to the purity and quality of the raw materials used during the development. So that, to achieve the next generation of devices is needed to overcome the limitations of state of art in terms of efficacy, biodegradability, and non-toxicity.

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Section: Biological Testing and Safety Assessmentmentioning

confidence: 99%

An Effective Translation: The Development of Hyaluronan-Based Medical Products From the Physicochemical, and Preclinical Aspects

Huerta-Ángeles

Nešporová

Ambrožová

et al. 2018

Front. Bioeng. Biotechnol.

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“…Previous attempts based on physical, chemical, and enzymatic crosslinking mechanisms to synthesize collagen/HA hybrid scaffolds involved a single‐step fabrication which usually yields homogeneous microstructure . On the other hand, our cross‐linking technique is a multistep approach based on carbodiimide chemistry to facilitate heterogeneous microstructure.…”

Section: Introductionmentioning

confidence: 99%

Collagen type I and hyaluronic acid based hybrid scaffolds for heart valve tissue engineering

et al. 2019

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Tissue engineers have achieved limited success so far in designing an ideal scaffold for aortic valve; scaffolds lack in mechanical compatibility, appropriate degradation rate, and microstructural similarity. This paper, therefore, has demonstrated a carbodiimide‐based sequential crosslinking technique to prepare aortic valve extracellular matrix mimicking (ECM) hybrid scaffolds from collagen type I and hyaluronic acid (HA), the building blocks of heart valve ECM, with tailorable crosslinking densities. Swelling studies revealed that crosslinking densities of parent networks increased with increasing the concentration of the crosslinking agents whereas crosslinking densities of hybrid scaffolds averaged from those of parent collagen and HA networks. Hybrid scaffolds also offered a wide range of pore size (66‐126 μm) which fulfilled the criteria for valvular tissue regeneration. Scanning electron microscopy and images of Alcian blue‐Periodic acid Schiff stained samples suggested that our crosslinking technique yielded an ECM mimicking microstructure with interlaced bands of collagen and HA in the hybrid scaffolds. The mutually reinforcing networks of collagen and HA also resulted in increased bending moduli up to 1660 kPa which spanned the range of natural aortic valves. Cardio sphere‐derived cells (CDCs) from rat hearts showed that crosslinking density affected the available cell attachment sites on the surface of the scaffold. Increased bending moduli of CDCs seeded scaffolds up to two folds (2‐6 kPa) as compared to the non‐seeded scaffolds (1 kPa) suggested that an increase in crosslinking density of the scaffolds could not only increase the in vitro bending modulus but also prevented its disintegration in the cell culture medium.

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“…The major functions of HA in this phase are the modulation of inflammatory cells and fibroblast cell migration, modulation of the synthesis of proinflammatory cytokines, and phagocytosis of invading microorganisms. In this phase, HA degradation products, such as low-molar-mass HA (2.5 × 10 5 Da), can enhance early inflammation [ 18 , 19 ]. The HA fragments, which accumulate due to degradation of high-molar-mass HA, can initiate toll receptor-2 and toll receptor-4 induction of pro-inflammatory cytokines IL-6, TNF-α, and IL-1β.…”

Section: Introductionmentioning

confidence: 99%

MitoQ Loaded Chitosan-Hyaluronan Composite Membranes for Wound Healing

et al. 2018

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Two self-associating biopolymers, namely chitosan (Ch) and a high-molar-mass hyaluronan (HA), were used to prepare membranes with the aim to protect and to enhance the healing of injured skin. A mitochondrially-targeted antioxidant—MitoQ—was incorporated into the mixture of biopolymers prior to their self-association. These three-component membranes were evaluated in detail utilising surface roughness measurements, contact angle measurements, hemocompatibility, and thrombogenicity analyses. Furthermore, in vivo application of Ch/HA/MitoQ membranes was assessed on injured rabbit and rat skin utilizing histological methods. The results showed that the prepared thrombogenic Ch/HA/MitoQ membranes had higher roughness, which allowed for greater surface area for tissue membrane interaction during the healing processes, and lower cytotoxicity levels than controls. MitoQ-loaded composite membranes displayed superior healing properties in these animal models compared to control membranes.

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Enhanced cell viability in hyaluronic acid coated poly(lactic-co-glycolic acid) porous scaffolds within microfluidic channels

Cited by 69 publications

References 43 publications

An Effective Translation: The Development of Hyaluronan-Based Medical Products From the Physicochemical, and Preclinical Aspects

An Effective Translation: The Development of Hyaluronan-Based Medical Products From the Physicochemical, and Preclinical Aspects

Collagen type I and hyaluronic acid based hybrid scaffolds for heart valve tissue engineering

MitoQ Loaded Chitosan-Hyaluronan Composite Membranes for Wound Healing

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