Enhanced Cellulosic Ethanol Production from Mild-Alkali Pretreated Rice Straw in SSF Using &lt;I&gt;Clavispora&lt;/I&gt; NRRL Y-50464

Chapla, Digantkumar; Parikh, Bhumika S.; Liu, Lewis Z.; Cotta, Michael A.; Kumar, Adepu K.

doi:10.1166/jbmb.2015.1533

Cited by 10 publications

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“…Y-50464 was evaluated on rice straw after different pretreatment procedures using mild-alkali, dilute-acid, or deep eutectic solvents. Ethanol production from mild-alkalipretreated rice straw SSFs was significantly higher than those from dilute-acid-pretreated rice straw SSFs at 10, 15, and 20% solids loadings [24]. For dilute-acid-pretreated rice straw, NRRL Y-50464 produced 16.8 g/L ethanol after 120 h from 15% solids loading.…”

Section: Rice Straw Ethanol Fermentation By Strain Nrrlmentioning

confidence: 89%

“…pretreated rice straw sugars. erefore, mild-alkali is preferred to dilute-acid for pretreating rice straw for ethanol conversion by SSF [24].…”

Section: Rice Straw Ethanol Fermentation By Strain Nrrlmentioning

confidence: 99%

“…Lignin has been well known as a major factor limiting enzymatic biomass deconstruction and higher titers of cellulosic ethanol production, and it was also observed from corn stover conversion using NRRL Y-50464 [21,57]. Mildalkali-and natural deep eutectic solvent-pretreated rice straw resulted in significantly higher ethanol production compared to dilute-acid-pretreated rice straw, which was due to the lignin removal by these methods [24,25]. A significant amount of glucan was also observed to be unavailable for ethanol conversion in corncob residues [36].…”

Section: Glucose-6pmentioning

confidence: 99%

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Cellulosic Ethanol Production Using a Dual Functional Novel Yeast

Liu

Dien

2022

International Journal of Microbiology

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Reducing the cost of cellulosic ethanol production, especially for cellulose hydrolytic enzymes, is vital to growing a sustainable and efficient cellulosic ethanol industry and bio-based economy. Using an ethanologenic yeast able to produce hydrolytic enzymes, such as Clavispora NRRL Y-50464, is one solution. NRRL Y-50464 is fast-growing and robust, and tolerates inhibitory compounds 2-furaldehyde (furfural) and 5-hydroxymethyl-2-furaldehyde (HMF) associated with lignocellulose-to-fuel conversion. It produces three forms of β-glucosidase isozymes, BGL1, BGL2, and BGL3, and ferment cellobiose as the sole carbon source. These β-glucosidases exhibited desirable enzyme kinetic parameters and high levels of enzyme-specific activity toward cellobiose and many oligosaccharide substrates. They tolerate the product inhibition of glucose and ethanol, and are stable to temperature and pH conditions. These characteristics are desirable for more efficient cellulosic ethanol production by simultaneous saccharification and fermentation. NRRL Y-50464 provided the highest cellulosic ethanol titers and conversion rates at lower cellulase loadings, using either pure cellulose or agricultural residues, as so far reported in the literature. This review summarizes NRRL Y-50464 performance on cellulosic ethanol production from refined cellulose, rice straw, and corn stover processed in various ways, in the presence or absence of furfural and HMF. This dual functional yeast has potential to serve as a prototype for the development of next-generation biocatalysts. Perspectives on continued strain development and process engineering improvements for more efficient cellulosic ethanol production from lignocellulosic materials are also discussed.

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Section: Rice Straw Ethanol Fermentation By Strain Nrrlmentioning

confidence: 89%

“…pretreated rice straw sugars. erefore, mild-alkali is preferred to dilute-acid for pretreating rice straw for ethanol conversion by SSF [24].…”

Section: Rice Straw Ethanol Fermentation By Strain Nrrlmentioning

confidence: 99%

Section: Glucose-6pmentioning

confidence: 99%

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Cellulosic Ethanol Production Using a Dual Functional Novel Yeast

Liu

Dien

2022

International Journal of Microbiology

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Improved cellulosic ethanol production from corn stover with a low cellulase input using a β-glucosidase-producing yeast following a dry biorefining process

Geberekidan

Zhang

Liu

et al. 2018

Bioprocess Biosyst Eng

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Technical Assessment of Cellulosic Ethanol Production Using β-Glucosidase Producing Yeast Clavispora NRRL Y-50464

Liu

Cotta

2015

Bioenerg. Res.

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Reducing the cost of cellulosic ethanol production, especially the use of expensive exogenous cellulose hydrolytic enzymes such as cellulase and β-glucosidase, is a critical challenge and vital for a sustainable advanced biofuel industry. Here, we report a novel ethanologenic yeast strain Clavispora NRRL Y-50464 that produces sufficient innate β-glucosidase enzyme activity for cellulosic ethanol production by simultaneous saccharification and fermentation (SSF). In a bottled SSF, strain Y-50464 produced 40.44 g/L ethanol from pure cellulose within 72 h at a conversion rate of 0.04 g/ L/h, applying conventional cellulase without supplementary β-glucosidase. Ethanol conversion from delignified corn stover by Y-50464 showed significantly higher titers and rates at various solids loading levels than that from conventional pretreated corn stover with over 40 to 60 % improved efficiency in a bottled SSF. However, the bottled SSF was inefficient for mixing higher levels of cellulose feedstock and should be replaced by a more suitable experimental apparatus. In a 2-L bioreactor SSF using conventional dilute acid pretreated corn stover, strain Y-50464 produced 32 g/L ethanol from 20 % solids loading at 48 h applying cellulase alone without addition of β-glucosidase. This represented a conversion rate of 0.088 g/L/h, the highest rate so far for cellulosic ethanol production from lignocellulosic materials. Elimination of β-glucosidase in cellulose-to-ethanol fermentation would be expected to reduce cost of cellulose conversion. The robustness, fast growth rate, and the capability of producing both ethanol and β-glucosidase illustrated the potential of strain Y-50464 as a potential candidate biocatalyst for advanced biofuel production from lignocellulosic biomass.

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Enhanced Cellulosic Ethanol Production from Mild-Alkali Pretreated Rice Straw in SSF Using <I>Clavispora</I> NRRL Y-50464

Cited by 10 publications

References 0 publications

Cellulosic Ethanol Production Using a Dual Functional Novel Yeast

Cellulosic Ethanol Production Using a Dual Functional Novel Yeast

Improved cellulosic ethanol production from corn stover with a low cellulase input using a β-glucosidase-producing yeast following a dry biorefining process

Technical Assessment of Cellulosic Ethanol Production Using β-Glucosidase Producing Yeast Clavispora NRRL Y-50464

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