Enhanced extraction of proteins using cholinium‐based ionic liquids as phase‐forming components of aqueous biphasic systems

Quental, Maria V.; Caban, Magda; Pereira, Matheus M.; Stepnowski, Piotr; Coutinho, João A. P.; Freire, Mara G.

doi:10.1002/biot.201500003

Cited by 98 publications

(125 citation statements)

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“…It was previously reported that [N 111(2OH) ]Cl is the salting out agent in PPG based ABS. 26 As shown in Figure 1A, the presence of a non salting out species, such as a carboxylic acid, seems to decrease the ability of ABS formation by [N 111(2OH) ]Cl. However, when the binodal curves are presented as a function of the isolated [N 111(2OH) ]Cl concentration, Figure 1C, there is an almost complete superposition of the solubility curves.…”

Section: ■ Results and Discussionmentioning

confidence: 94%

Are Aqueous Biphasic Systems Composed of Deep Eutectic Solvents Ternary or Quaternary Systems?

Passos

Tavares

Ferreira

et al. 2016

ACS Sustainable Chem. Eng.

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Deep eutectic solvents (DES) have emerged in the past few years as a new class of solvents with promising applications in several fields. In the present work, the application of DES (formed by binary mixtures of cholinium chloride and carboxylic acids or urea) as phase forming components of aqueous biphasic systems (ABS) is inves tigated. The mechanisms associated with the phases demixing of ABS composed of DES, as well as the DES stability in aqueous solutions, are investigated to address the critical question whether DES based ABS are in fact ternary or quaternary mixtures. It is shown that the DES integrity is destroyed in ABS by the disruption of the hydrogen bonding interactions of the complex (a result of the isolated components preferential solvation by water), and as confirmed by a nonstoichiometric partition of the DES components between the coexisting phases. As a result, there are no "real" DES based ABS; instead, there is the formation of ABS composed of four components, where the carboxylic acid used as the hydrogen bond donor species seems to act as an additive. Finally, it is shown that these ABS have an outstanding potential to be used in extraction processes, as it is here demonstrated with the complete separation of two dyes. However, the volatile nature of short chain carboxylic acids and the nonstoichiometric partition of the DES components in ABS make the development of recovery and recycling steps more difficult to accomplish. The formation of DES results from the formation of strong hydrogen bonds between the two starting materials, namely, a hydrogen bond donor (HBD) and a hydrogen bond acceptor (HBA). The formation of ion−HBD complexes leads to a lower entropic difference of the phase transition and to the further depression of the freezing temperature.5−7 The first reported DES (composed of [N 111(2OH) ]Cl and urea) 1 remains the most widely investigated. However, DES can be formed by mixing other substituted tetralkylammonium or phosphonium salts with a HBD, such as amines, carboxylic acids, and carbohydrates, among others. 8−10Many DES share some of the unique characteristics of ionic liquids (ILs), namely, a low volatility, high conductivity, wide liquid temperature range, and high solvation ability for a large number of compounds.3,4 DES are also able to overcome some of the disadvantages related to ILs. They are easier to prepare, by a simple mixture of the starting materials at moderate temperatures, without requiring a reaction step. Furthermore, the addition of an agent able to disrupt hydrogen bonding interactions can lead to the recovery of at least one of the initial compounds. 3,4,11,12 The starting materials are usually cheaper, toxicologically well characterized, and often derived from renewable resources, which results in low cost and more environmentally friendly products. 3,4,11,12 As in ILs, innumer ous combinations of the starting materials can be attempted, and therefore, DES may also be classified as "designer solvents". Since DES have fewer restrictions ...

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Section: ■ Results and Discussionmentioning

confidence: 94%

Are Aqueous Biphasic Systems Composed of Deep Eutectic Solvents Ternary or Quaternary Systems?

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Tavares

Ferreira

et al. 2016

ACS Sustainable Chem. Eng.

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“…The hydration of the bottom phase main component also is important in the separation of hydrophobic molecules . In the case of PEG400‐Ch DHp, the high affinity of the IL for water may lead to a higher partitioning of pigments to the top phase. However, interactions between the polymer and the IL play an important role in the partitioning of biomolecules, making the partitioning of the molecules more difficult to predict .…”

Section: Resultsmentioning

confidence: 99%

“…In the PEG400‐Ch DHp system, proteins were concentrated mainly in the interface and in the bottom phase when using both cultivation conditions. Previous studies have reported the preference of pure proteins to the bottom phase when using ATPS combinations with a more hydrophobic polymer (PPG400) and cholinium‐based ILs . Li et al .…”

Section: Resultsmentioning

confidence: 99%

Selective and mild fractionation of microalgal proteins and pigments using aqueous two‐phase systems

Ruiz

Emmery

Wijffels

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUNDMicroalgal biomass is generally used to produce a single product instead of valorizing all of the cellular components. The biomass production and downstream processes are too expensive if only one product is valorized. A new approach was proposed for the simultaneous and selective partitioning of pigments and proteins from disrupted Neochloris oleoabundans cultivated under saline and freshwater conditions.RESULTSAn aqueous two‐phase system composed of polyethylene glycol and cholinium dihydrogen phosphate selectively separated microalgal pigments from microalgal proteins. 97.3 ± 1.0% of lutein and 51.6 ± 2.3% of chlorophyll were recovered in the polymer‐rich phase. Simultaneously, up to 92.2 ± 2.0% of proteins were recovered in a third phase (interface) in between the aqueous phases (interface). The recovered proteins, including Rubisco with a molecular weight of ∼560 kDa, seem to be intact and pigments did not suffer degradation, demonstrating the mildness of this system for fractionating microalgal biomolecules.CONCLUSIONThe ability of aqueous two‐phase systems (ATPSs) to simultaneously and efficiently fractionate different biomolecules in a mild manner from disrupted microalgae is demonstrated. This is an important step towards the development of a multiproduct microalgae biorefinery. © 2018 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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“…Other useful advantages of these substances over common organic solvents are their non-flammability, low vapor pressure, and thermal stability. These compounds are synthesized by either cationic/anionic exchange (Bonhôte et al, 1996) or by the neutralization reaction of an acid and a base (Quental et al, 2015). Ionic liquids are also regarded as designer solvents since their properties can be easily changed by modifying its cation or anion.…”

Section: Ionic Liquid Extractionmentioning

confidence: 99%

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

Chua

Schenk

2017

Bioresource Technology

131

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Please cite this article as: Chua, E.T., Schenk, P.M., A biorefinery for Nannochloropsis: induction, harvesting, and extraction of EPA-rich oil and high-value protein, Bioresource Technology (2017), doi: http://dx.doi.org/10. 1016/ j.biortech.2017.05.124 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractMicroalgae have been studied as biofactories for almost four decades. Yet, even until today, many aspects of microalgae farming and processing are still considered exploratory because of the uniqueness of each microalgal species. Thus, it is important to develop the entire process of microalgae farming: from culturing to harvesting, and down to extracting the desired high-value products. Based on its rapid growth and high oil productivities, Nannochloropsis sp. is of particular interest to many industries for the production of highvalue oil containing omega-3 fatty acids, specifically eicosapentaenoic acid (EPA), but also several other products. This review compares the various techniques for induction, harvesting, and extraction of EPA-rich oil and high-value protein explored by academia and industry to develop a multi-product Nannochloropsis biorefinery. Knowledge gaps and opportunities are discussed for culturing and inducing fatty acid biosynthesis, biomass harvesting, and extracting EPA-rich oil and high-value protein from the biomass of Nannochloropsis sp.

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Enhanced extraction of proteins using cholinium‐based ionic liquids as phase‐forming components of aqueous biphasic systems

Cited by 98 publications

References 79 publications

Are Aqueous Biphasic Systems Composed of Deep Eutectic Solvents Ternary or Quaternary Systems?

Are Aqueous Biphasic Systems Composed of Deep Eutectic Solvents Ternary or Quaternary Systems?

Selective and mild fractionation of microalgal proteins and pigments using aqueous two‐phase systems

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

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