Enhanced gene expression in mouse lung by prolonging the retention time of intravenously injected plasmid DNA

Song, Young K.; Liu, F; Liu, D

doi:10.1038/sj.gt.3300770

Cited by 85 publications

(37 citation statements)

References 23 publications

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“…Interestingly, the large increase in luciferase activity was obtained with either DOTAP/pCMV␤n or DOTAP/pCLuc complexes used as the first dose. These results are similar to those obtained by pre-injecting cationic lipids and subsequently either naked DNA 34 or DNA/lipid complexes. 35 A possible explanation is that aggregates between DNA complexes and serum proteins are trapped in lung capillaries and thereby retain the DNA complexes injected later.…”

Section: Figure 6 Identification Of Transfected Lung Cells In Vivo Fosupporting

confidence: 87%

Comparison between cationic polymers and lipids in mediating systemic gene delivery to the lungs

et al. 1999

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Airway inflammation frequently found in congenital and mice efficiently transfected primarily the lungs and to a acquired lung diseases may interfere with gene delivery by lesser extent, heart, spleen, kidney and liver. The other direct administration through either instillation or aerosol.vectors mediated lower to undetectable levels of luciferase Systemic delivery by the intravenous administration repexpression in the lungs, with DOTAP Ͼ GL67/DOPE Ͼ PEI resents an alternative route of delivery that might bypass 25K Ͼ DOTMA/DOPE. A double injection protocol with a this barrier. A nonviral approach for transfecting various 15-min interval between the two doses of DOTAP/DNA airway-derived cell lines in vitro showed that cationic polycomplexes was investigated and showed a relevant role of mers (PEI 22K and 25K) and lipids (DOTAP, GL-67/DOPE) the first injection in transfecting the lungs. A two log are able to transfect with high efficiency the reporter genes increase in luciferase expression was obtained either when firefly luciferase and E. coli lacZ. Notably, two properties the two doses were comprised of luciferase plasmid or predicted that cationic vectors would be useful for a syswhen an irrelevant plasmid was used in the first injection. temic gene delivery approach to the lung: (1) transfectionThe double injection of luciferase/PEI 22K complexes was not inhibited or increased when cells were incubated determined higher transgene levels than a single dose, but with cationic lipids or polymers in the presence of serum; a clear difference using an irrelevant plasmid as first dose and (2) cationic vectors protected plasmid DNA from was not observed. Using lacZ as a reporter gene, it was DNase degradation. A single injection of DNA complexed shown that only cells in the alveolar region, including type II to the cationic polymer PEI 22K into the tail vein of adult penumocytes, stained positively for the transgene product.

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Section: Figure 6 Identification Of Transfected Lung Cells In Vivo Fosupporting

confidence: 87%

Comparison between cationic polymers and lipids in mediating systemic gene delivery to the lungs

et al. 1999

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“…During the first hour, there was little change in this distribution, except a progressive NP release from lungs to liver and spleen. Two reasons can explain this biodistribution pattern (17). The first one is a nonspecific electrostatic NP interaction with the negative charges displayed by plasmatic membranes of capillary endothelial cells, such as sulfated proteoglycans and glycosylaminoglycans.…”

Section: Resultsmentioning

confidence: 99%

Nanoprobes with near-infrared persistent luminescence for in vivo imaging

Chermont

Chanéac

Séguin

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

763

501

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Fluorescence is increasingly used for in vivo imaging and has provided remarkable results. Yet this technique presents several limitations, especially due to tissue autofluorescence under external illumination and weak tissue penetration of low wavelength excitation light. We have developed an alternative optical imaging technique by using persistent luminescent nanoparticles suitable for small animal imaging. These nanoparticles can be excited before injection, and their in vivo distribution can be followed in real-time for more than 1 h without the need for any external illumination source. Chemical modification of the nanoparticles' surface led to lung or liver targeting or to long-lasting blood circulation. Tumor mass could also be identified on a mouse model. biodistribution ͉ in vivo optical imaging ͉ nanoparticles ͉ phosphorescent nanoparticles

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“…Recently, it was shown that intravenous administration of naked plasmid DNA produced certain amount of gene expression in the liver (8) and the lung (9), although efficiency of this process is relatively low. Song et al (9) reported that pretreatment of cationic liposomes (DOTAP) enhanced transfection efficiency of systemically injected naked plasmid DNA (50 g/20 g of a mouse) into mouse lung. They proposed that pretreated cationic liposome play a role in formation of transient microembolization in pulmonary capillary beds and prolong retention time of naked plasmid DNA that may enhance gene transfer efficiency due to providing longer time to plasmid DNA to contact with host cells.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Expressions and Histological Characteristics of Intravenously Administered Plasmid DNA in Rat Lung

Rha

2001

J Korean Med Sci

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INTRODUTIONIntravenous delivery of therapeutic genes for gene therapy has an advantage for lung tumor that is deeply located in the thorax. But there are few successful methods for intravenous delivery of genes into the lung. Several methods using viral (1, 2) and liposomal (3-7) vectors are now studied intensively for intravenous delivery of genes into the lung. Templeton et al. (6) demonstrated that DOTAP-cholesterol-plasmid DNA complex showed high affinity in mouse lung after intravenous infusion. DOTAP is a well-known cationic lipid for making lipid-DNA complex and is used in many gene transfection researches. Recently, the ability for intravenously administered naked plasmid DNA to be expressed at substantial levels in mammalian organs was demonstrated (8-10). Also several supportive methods for enhancement of intravenously administered naked plasmid DNA transfection were introduced. Song et al. (9) demonstrated that intravenously pretreated DOTAP, which formed transient microembolism on pulmonary capillary beds, prolonged the retention time of naked plasmid DNA and produced more transfection efficiency of plasmid DNA. Liu et al. (10) demonstrated that rapid intravenous injection of a large volume of naked plasmid DNA solution produced efficient gene transfection. In this study, we have compared the transfection efficiencies and histological characteristics after intravenous administration 1) of naked plasmid DNA alone, 2) naked plasmid DNA after DOTAP pretreatment, and 3) DOTAPcholesterol-plasmid DNA complex.

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Enhanced gene expression in mouse lung by prolonging the retention time of intravenously injected plasmid DNA

Cited by 85 publications

References 23 publications

Comparison between cationic polymers and lipids in mediating systemic gene delivery to the lungs

Comparison between cationic polymers and lipids in mediating systemic gene delivery to the lungs

Nanoprobes with near-infrared persistent luminescence for in vivo imaging

Enhanced Expressions and Histological Characteristics of Intravenously Administered Plasmid DNA in Rat Lung

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