Enhanced heat and drought tolerance in transgenic rice seedlings overexpressing OsWRKY11 under the control of HSP101 promoter

Wu, Xiujie; Shiroto, Yoko; Kishitani, Sachie; Ito, Yukihiro; Toriyama, Kinya

doi:10.1007/s00299-008-0614-x

Cited by 459 publications

(249 citation statements)

References 49 publications

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“…The regulation of stress-related genes with constitutive promoters (i.e., CaMV35S promoter, actin promoter, and ubiquitin promoter) in the plant biotechnology industry is considered undesirable as it may lead to unwanted and hence wasteful metabolisms, causing ultimately a reduction in the growth and yield of transgenic plants (Ito et al 2006). Wu et al (2009) employed OsHsp100 promoter for driving OsWRKY11 gene for imparting desiccation tolerance to rice seedlings.…”

Section: Discussionmentioning

confidence: 99%

OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB (Hsp100) gene in rice (Oryza sativa L.)

Singh

Mittal

Lavania

et al. 2012

Cell Stress and Chaperones

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ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an important chaperone protein in rice. Cellular expression of OsClpB-cyt transcript is governed by heat stress, metal stress, and developmental cues. Transgenic rice plants produced with 2 kb OsClpB-cyt promoter driving Gus reporter gene showed heat-and metal-regulated Gus expression in vegetative tissues and constitutive Gus expression in calli, flowering tissues, and embryonal half of seeds. Rice seedlings regenerated with OsClpB-cyt promoter fragment with deletion of its canonical heat shock element sequence (HSE −273 to −280 ) showed not only heat shock inducibility of Gus transcript/protein but also constitutive expression of Gus in vegetative tissues. It thus emerges that the only classical HSE present in OsClpB-cyt promoter is involved in repressing expression of OsClpB-cyt transcript under unstressed control conditions. Yeast onehybrid assays suggested that OsHsfA2c specifically interacts with OsClpB-cyt promoter. OsHsfA2c also showed binding with OsClpB-cyt and OsHsfB4b showed binding with OsClpB-cyt; notably, interaction of OsHsfB4b was seen for all three OsClpB/Hsp100 protein isoforms (i.e., ClpBcytoplasmic, ClpB-mitochondrial, and ClpB-chloroplastic).Furthermore, OsHsfB4b showed interaction with OsHsfA2c. This study suggests that OsHsfA2c may play a role as transcriptional activator and that OsHsfB4b is an important part of this heat shock responsive circuitry.

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Section: Discussionmentioning

confidence: 99%

OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB (Hsp100) gene in rice (Oryza sativa L.)

Singh

Mittal

Lavania

et al. 2012

Cell Stress and Chaperones

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“…Stunted growth is the common phenotype observed when a TF gene is overexpressed in transgenic plants. This has been observed in soybean (Barbosa et al, 2013), tomato (Pan et al, 2012), rice (Wu et al, 2009), tobacco (Kasuga et al, 2004), and chrysanthemum (Hong et al, 2006). With the selection of different promoters, it is possible to express transgenes in defined temporal and spatial manners.…”

Section: Mechanisms Of Regulation Of Different Classes Of Tfsmentioning

confidence: 99%

“…Heterologous overexpression of OsWRKY45 in Arabidopsis resulted in enhanced resistance to the bacterial pathogen Pseudomonas syringae and improved tolerance to salt and drought stresses in transgenic plants (Qiu and Yu, 2009). Overexpression of OsWRKY11 driven by an HSP101 promoter conferred enhanced tolerance to heat and drought in seedlings after heat pretreatment at 37/22°C (12 h/12 h) for 2 weeks (Wu et al, 2009). This again illustrates the importance of promoter choice in strategies that use transgenes to confer drought tolerance.…”

Section: Wrky Tf Familymentioning

confidence: 99%

The Potential of Transcription Factor-Based Genetic Engineering in Improving Crop Tolerance to Drought

Rabara¹,

Tripathi

Rushton³

2014

OMICS: A Journal of Integrative Biology

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Drought is one of the major constraints in crop production and has an effect on a global scale. In order to improve crop production, it is necessary to understand how plants respond to stress. A good understanding of regulatory mechanisms involved in plant responses during drought will enable researchers to explore and manipulate key regulatory points in order to enhance stress tolerance in crops. Transcription factors (TFs) have played an important role in crop improvement from the dawn of agriculture. TFs are therefore good candidates for genetic engineering to improve crop tolerance to drought because of their role as master regulators of clusters of genes. Many families of TFs, such as CCAAT, homeodomain, bHLH, NAC, AP2/ERF, bZIP, and WRKY have members that may have the potential to be tools for improving crop tolerance to drought. In this review, the roles of TFs as tools to improve drought tolerance in crops are discussed. The review also focuses on current strategies in the use of TFs, with emphasis on several major TF families in improving drought tolerance of major crops. Finally, many promising transgenic lines that may have improved drought responses have been poorly characterized and consequently their usefulness in the field is uncertain. New advances in high-throughput phenotyping, both greenhouse and field based, should facilitate improved phenomics of transgenic lines. Systems biology approaches should then define the underlying changes that result in higher yields under water stress conditions. These new technologies should help show whether manipulating TFs can have effects on yield under field conditions.

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“…While overexpression of GmWRKY21 in Arabidopsis increased cold tolerance, transgenic Arabidopsis plants overexpressing GmWRKY54 gene enhanced drought tolerance (Zhou et al 2008). In rice seedlings, overexpression of OsWRKY11 gene improved drought tolerance (Wu et al 2009). These findings confirm the involvement of WRKY genes in the regulation of abiotic stress response in different species.…”

Section: Studies Involving Individual Wrky Tfs Inmentioning

confidence: 99%

Identification of a drought- and cold-stress inducibleWRKYgene in the cold-hardyCitrusrelativePoncirus trifoliata

Şahin‐Çevik

Moore

2013

New Zealand Journal of Crop and Horticultural Science

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WRKY transcription factors (TFs) are involved in regulation of abiotic and biotic stress responsive genes and play a role in abiotic and biotic stress tolerance in plants. Although commercial Citrus species are sensitive to cold and drought stresses, an interfertile Citrus relative, Poncirus trifoliata, is resistant to cold and more drought tolerant than most commercial Citrus cultivars. An expressed sequence tag clone sharing homology with WRKY-type TFs was previously isolated from a cDNA library constructed using two-day cold-acclimated P. trifoliata cv. Rubidoux seedlings. In this study, a full-length sequence of this WRKY gene was cloned and isolated from 2-day cold-acclimated P. trifoliata plants using the rapid amplification of cDNA ends (RACE) method. The full-length PtrWRKY1 cDNA is 1807 bp and encodes a polypeptide of 405 amino acids containing a single WRKY domain with a Cys 2 His 2 motif, which is a signature of WRKY TFs. A homologous open reading frame (ORF) sequence was isolated from Citrus grandis, CgWRKY1, sharing 98% identity at the nucleotide level with PtrWRKY1. The expression of PtrWRKY1 and CgWRKY1 was investigated in response to cold and drought stresses by northern blot analysis. While the expression of both genes was induced in response to drought, only PtrWRKY1 expression was induced in response to cold. This differential expression in cold-hardy Poncirus and cold-sensitive pummelo suggests that PtrWRKY1 gene may play a role in adaptation and tolerance to cold stress in Poncirus.

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Enhanced heat and drought tolerance in transgenic rice seedlings overexpressing OsWRKY11 under the control of HSP101 promoter

Cited by 459 publications

References 49 publications

OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB (Hsp100) gene in rice (Oryza sativa L.)

OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB (Hsp100) gene in rice (Oryza sativa L.)

The Potential of Transcription Factor-Based Genetic Engineering in Improving Crop Tolerance to Drought

Identification of a drought- and cold-stress inducibleWRKYgene in the cold-hardyCitrusrelativePoncirus trifoliata

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