Enhanced <i>in situ</i> detection and imaging of lipids in biological tissues by using 2,3-dicyanohydroquinone as a novel matrix for positive-ion MALDI-MS imaging

Liu, Yaqin; Chen, Lulu; Qin, Liang; Han, Manman; Li, Jinming; Luo, Fei‐Xian; Xue, Kun; Feng, Jinchao; Zhou, Yijun; Wang, Xinli

doi:10.1039/c9cc06961e

Cited by 33 publications

(29 citation statements)

References 20 publications

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“…According to previous studies, , the absorption spectra of the CA, SA, and FA solutions were measured on a Jasco UV–vis spectrometer (Model V-550, Tokyo, Japan). Solid-phase UV–vis absorption spectra of CA, SA, and FA were acquired on a UV-3600 Plus spectrophotometer (Shimadzu, Japan).…”

Section: Methodsmentioning

confidence: 99%

A Caffeic Acid Matrix Improves In Situ Detection and Imaging of Proteins with High Molecular Weight Close to 200,000 Da in Tissues by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

Liu

Han

et al. 2021

Anal. Chem.

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To our knowledge, this was the first study in which caffeic acid (CA) was successfully evaluated as a matrix to enhance the in situ detection and imaging of endogenous proteins in three biological tissue sections (i.e., a rat brain and Capparis masaikai and germinating soybean seeds) by matrix-assisted laser desorption/ ionization mass spectrometry imaging (MALDI-MSI). Our results show several properties of CA, including strong ultraviolet absorption, a super-wide MS detection mass range close to 200,000 Da, micrometer-sized matrix crystals, uniform matrix deposition, and high ionization efficiency. More high-molecularweight (HMW) protein ion signals (m/z > 30,000) could be clearly detected in biological tissues with the use of CA, compared to two commonly used MALDI matrices, i.e., sinapinic acid (SA) and ferulic acid (FA). Notably, CA shows excellent performance for HMW protein in situ detection from biological tissues in the mass range m/z > 80,000, compared to the use of SA and FA. Furthermore, the use of a CA matrix also significantly enhanced the imaging of proteins on the surface of selected biological tissue sections. Three HMW protein ion signals (m/z 50,419, m/z 65,874, and m/z 191,872) from a rat brain, two sweet proteins (mabinlin-2 and mabinlin-4) from a Capparis masaikai seed, and three HMW protein ion signals (m/z 94,838, m/z 134,204, and m/z 198,738) from a germinating soybean seed were successfully imaged for the first time. Our study proves that CA has the potential to become a standard organic acid matrix for enhanced tissue imaging of HMW proteins by MALDI-MSI in both animal and plant tissues.

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Section: Methodsmentioning

confidence: 99%

A Caffeic Acid Matrix Improves In Situ Detection and Imaging of Proteins with High Molecular Weight Close to 200,000 Da in Tissues by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

Liu

Han

et al. 2021

Anal. Chem.

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“…2,3-Dicyanohydroquinone (DCH) is originally known as a fluorescent dye and was applied by Liu and coworkers [41] as a new matrix for imaging of lipids in biological tissues. In contrast to commonly used matrices, more lipids could be detected from mouse brain and germinating Chinese yew seed tissue sections in positive ion mode.…”

Section: Rationally Designed and Synthesized Novel Matricesmentioning

confidence: 99%

Recent developments of novel matrices and on-tissue chemical derivatization reagents for MALDI-MSI

2020

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Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a fast-growing technique for visualization of the spatial distribution of the small molecular and macromolecular biomolecules in tissue sections. Challenges in MALDI-MSI, such as poor sensitivity for some classes of molecules or limited specificity, for instance resulting from the presence of isobaric molecules or limited resolving power of the instrument, have encouraged the MSI scientific community to improve MALDI-MSI sample preparation workflows with innovations in chemistry. Recent developments of novel small organic MALDI matrices play a part in the improvement of image quality and the expansion of the application areas of MALDI-MSI. This includes rationally designed/synthesized as well as commercially available small organic molecules whose superior matrix properties in comparison with common matrices have only recently been discovered. Furthermore, on-tissue chemical derivatization (OTCD) processes get more focused attention, because of their advantages for localization of poorly ionizable metabolites and their‚ in several cases‚ more specific imaging of metabolites in tissue sections. This review will provide an overview about the latest developments of novel small organic matrices and on-tissue chemical derivatization reagents for MALDI-MSI.

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“…To ensure and improve the ionization efficiency of 2-HG from glioma tissue microenvironment for the detection on a MALDI mass spectrometer, the selection and application of matrix is one of the most essential steps. 28 In a previous study, 1,5-DAN was reported as the most efficient matrix to detect 2-HG in glioma tissues compared to other matrices, including 2,5-dihydroxybenzoic acid (DHB), maleic anhydride proton sponge (MAPS) and 9-aminoacridine (9-AA). 20 However, 1,5-DAN is a well-known cancerogen.…”

Section: Selection and Optimization Of Matrix For 2-hg Detectionmentioning

confidence: 99%

Absolute quantification of 2‐hydroxyglutarate on tissue by matrix‐assisted laser desorption/ionization mass spectrometry imaging for rapid and precise identification of isocitrate dehydrogenase mutations in human glioma

Lan

Wang

et al. 2021

Intl Journal of Cancer

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Isocitrate dehydrogenase (IDH) gene mutations are important predictive molecular markers to guide surgical strategy in brain cancer therapy. Herein, we presented a method using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) for absolute quantification of 2-hydroxyglutarate (2-HG) on tissues to identify IDH mutations and evaluate tumor residue. This analytical method was tested among 34 glioma patients and validated with gold standard clinical technologies. The cut-off value of 2-HG was set as 0.81 pmol/μg to identify IDH mutant (IDHmt) gliomas with 100% specificity and sensitivity. In addition, 2-HG levels and tumor cell density (TCD) showed positive correlation in IDHmt gliomas by this spatial method. This MALDI MSI-based absolute quantification method has great potentiality for incorporating into surgical workflow in the future.

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Enhanced in situ detection and imaging of lipids in biological tissues by using 2,3-dicyanohydroquinone as a novel matrix for positive-ion MALDI-MS imaging

Abstract: DCH (2,3-dicyanohydroquinone), a fluorescent dye, was reported as a novel and powerful (+) MALDI matrix for lipid tissue imaging.

Cited by 33 publications

References 20 publications

A Caffeic Acid Matrix Improves In Situ Detection and Imaging of Proteins with High Molecular Weight Close to 200,000 Da in Tissues by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

A Caffeic Acid Matrix Improves In Situ Detection and Imaging of Proteins with High Molecular Weight Close to 200,000 Da in Tissues by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

Recent developments of novel matrices and on-tissue chemical derivatization reagents for MALDI-MSI

Absolute quantification of 2‐hydroxyglutarate on tissue by matrix‐assisted laser desorption/ionization mass spectrometry imaging for rapid and precise identification of isocitrate dehydrogenase mutations in human glioma

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