Enhanced immune responses and effectiveness of refined outer membrane protein vaccines against <i>Vibrio harveyi</i> in orange‐spotted grouper (<i>Epinephelus coioides</i>)

Nguyen, Hai Trong; Nguyen, Thuy Thi Thu; Chen, Yan-Chun; Vu-Khac, Hung; Wang, Pei‐Chi; Chen, Shih‐Chu

doi:10.1111/jfd.12828

Cited by 22 publications

(8 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…injection (dose = 100 µl/fish) estuary cod (Epinephelus coioides) leading to an RPS or 60% or 70% depending on the challenge culture. A recombinant fusion protein r-OmpK-OmpU controlled disease in orange-spotted grouper (RPS = 81.8%) (Nguyen et al 2018). Subsequently, the OMP To1C, which is a major adhesin, was used successfully to protect hybrid grouper (E. fuscoguttatus × E. lanceolatus) against experimental challenge (Zhu et al 2019).…”

Section: Vaccinesmentioning

confidence: 99%

Vibrio harveyi: a serious pathogen of fish and invertebrates in mariculture

Zhang

Austin

2020

Mar Life Sci Technol

205

View full text Add to dashboard Cite

Vibrio harveyi, which belongs to family Vibrionaceae of class Gammaproteobacteria, includes the species V. carchariae and V. trachuri as its junior synonyms. The organism is a well-recognized and serious bacterial pathogen of marine fish and invertebrates, including penaeid shrimp, in aquaculture. Diseased fish may exhibit a range of lesions, including eye lesions/blindness, gastro-enteritis, muscle necrosis, skin ulcers, and tail rot disease. In shrimp, V. harveyi is regarded as the etiological agent of luminous vibriosis in which affected animals glow in the dark. There is a second condition of shrimp known as Bolitas negricans where the digestive tract is filled with spheres of sloughed-off tissue. It is recognized that the pathogenicity mechanisms of V. harveyi may be different in fish and penaeid shrimp. In shrimp, the pathogenicity mechanisms involved the endotoxin lipopolysaccharide, and extracellular proteases, and interaction with bacteriophages. In fish, the pathogenicity mechanisms involved extracellular hemolysin (encoded by duplicate hemolysin genes), which was identified as a phospholipase B and could inactivate fish cells by apoptosis, via the caspase activation pathway. V. harveyi may enter the so-called viable but nonculturable (VBNC) state, and resuscitation of the VBNC cells may be an important reason for vibriosis outbreaks in aquaculture. Disease control measures center on dietary supplements (including probiotics), nonspecific immunostimulants, and vaccines and to a lesser extent antibiotics and other antimicrobial compounds.

show abstract

Section: Vaccinesmentioning

confidence: 99%

Vibrio harveyi: a serious pathogen of fish and invertebrates in mariculture

Zhang

Austin

2020

Mar Life Sci Technol

205

View full text Add to dashboard Cite

show abstract

“…Although other antigens have been pointed out as vaccine candidates, like TolC, an outer membrane protein from V. harveyi ( 85 ) and DNAj ( 86 ), several experimental vaccines using OmpK were described as efficient. For instance, vaccination of Orange-spotted groupers with a recombinant OmpK induced a higher tolerance to Vibrio infections ( 83 ); a subunit vaccine involving OmpK and OmpU fusion resulted in a protective effect against V. harveyi infection and showed potential against other species ( 87 ); E. coli based recombinant vaccines containing OmpK were efficient against challenge with multiple strains of V. harveyi , V. alginolyticus , and V. parahaemolyticus ( 86 ). Nonetheless, studies regarding either the immunogenicity of OmpK or the cross-protection effect of recombinant OmpK vaccines are not consistent.…”

Section: Discussionmentioning

confidence: 99%

Oral vaccination of fish against vibriosis using spore-display technology

et al. 2022

View full text Add to dashboard Cite

Oral vaccines are highly demanded by the aquaculture sector, to allow mass delivery of antigens without using the expensive and labor-intensive injectable vaccines. These later require individual handling of fish, provoking stress-related mortalities.One possible strategy to create injection-free vaccine delivery vehicles is the use of bacterial spores, extremely resistant structures with wide biotechnological applications, including as probiotics, display systems, or adjuvants. Bacterial spores, in particular those of Bacillus subtilis, have been shown to behave as mucosal vaccine adjuvants in mice models. However, such technology has not been extensively explored against fish bacterial disease.In this study, we used a laboratory strain of B. subtilis, for which a variety of genetic manipulation tools are available, to display at its spores surface either a Vibrio antigenic protein, OmpK, or the green fluorescence protein, GFP. When previously vaccinated by immersion with the OmpK- carrying spores, zebrafish survival upon a bacterial challenge with V. anguillarum and V. parahaemolyticus, increased up to 50 - 90% depending on the pathogen targeted. Further, we were able to detect anti-GFP-antibodies in the serum of European seabass juveniles fed diets containing the GFP-carrying spores and anti-V. anguillarum antibodies in the serum of European seabass juveniles fed the OmpK-carrying spores containing diet. More important, seabass survival was increased from 60 to 86% when previously orally vaccinated with in-feed OmpK- carrying spores. Our results indicate that B. subtilis spores can effectively be used as antigen-carriers for oral vaccine delivery in fish.

show abstract

“…The high immunogenicity of outer membrane proteins (OMP) is considered a promising candidate for the design of antimicrobial drugs and vaccines in various pathogenic strains. Numerous other studies have reported that outer membrane proteins (OMPs) are generally very immunogenic due to their exposed epitopes on the cell surface ( 176 ) and have the potential of a vaccine candidate for fish against V. anguillarum ( 177 , 178 ), Vibrio mimicus ( 179 ), V. harveyi ( 180 ), Vibrio ichthyoenteri ( 181 ), P. olivaceus ( 182 ), E. tarda ( 183 ) and A. hydrophila ( 184 ) infection. Flagellin is also well-studied and effective subunit vaccine candidate ( 185 ).…”

Section: Classification and Preparation Technology Of Aquatic Vaccinesmentioning

confidence: 99%

Current status and development prospects of aquatic vaccines

Liu

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

Diseases are a significant impediment to aquaculture’s sustainable and healthy growth. The aquaculture industry is suffering significant financial losses as a result of the worsening water quality and increasing frequency of aquatic disease outbreaks caused by the expansion of aquaculture. Drug control, immunoprophylaxis, ecologically integrated control, etc. are the principal control strategies for fish infections. For a long time, the prevention and control of aquatic diseases have mainly relied on the use of various antibiotics and chemical drugs. However, long-term use of chemical inputs not only increases pathogenic bacteria resistance but also damages the fish and aquaculture environments, resulting in drug residues in aquatic products, severely impeding the development of the aquaculture industry. The development and use of aquatic vaccines are the safest and most effective ways to prevent aquatic animal diseases and preserve the health and sustainability of aquaculture. To give references for the development and implementation of aquatic vaccines, this study reviews the development history, types, inoculation techniques, mechanisms of action, development prospects, and challenges encountered with aquatic vaccines.

show abstract

Enhanced immune responses and effectiveness of refined outer membrane protein vaccines against Vibrio harveyi in orange‐spotted grouper (Epinephelus coioides)

Cited by 22 publications

References 39 publications

Vibrio harveyi: a serious pathogen of fish and invertebrates in mariculture

Vibrio harveyi: a serious pathogen of fish and invertebrates in mariculture

Oral vaccination of fish against vibriosis using spore-display technology

Current status and development prospects of aquatic vaccines

Contact Info

Product

Resources

About