Enhanced in vitro caulogenesis and quantitative estimation of aloin through HPLC in Aloe vera

Abstract

Aloe vera (Liliaceae) is well known for its medicinal properties. It contains flavonoids, saponins, tannins, alkaloids and other compounds which exhibit medicinal, cosmetic and pharmacological properties. There is a lack of production of aloe leaf to meet the industry demand and therefore the means to facilitate large-scale aloe cultivation schemes need to be developed. Because sexual reproduction by seeds is almost ineffective in Aloe vera due to male sterility and vegetative propagation by offshoots is only possible during the growing seasons, there is a need to develop a propagation method to facilitate large-scale cultivation. In vitro regeneration of medicinal plants is important and there is the potential for the production of high-quality plant-based medicine. In the present study, an attempt has been made to enhance in vitro caulogenesis and aloin content in plants in the presence of a precursor (tryptophan) in the nutrient media. For in vitro culture establishment and shoot bud multiplication, MS basal media were used supplemented with different concentrations and different combinations of growth regulators such as BAP (6-benzylaminopurine) and IAA (3-indole acetic acid). Shoot proliferation was optimal in MS medium containing 2.0 mg l ¡1 BAP. For evaluation of in vitro enhancement of aloin production the precursor tryptophan was added to the nutrient media at different levels (5, 10, 15 and 20 mg l ¡1 ). Addition of 20 mg l ¡1 tryptophan induced a 2.43-fold increase in aloin content in multiple shoots cultures of Aloe barbadensis.

Propagation Techniques and Agronomic Requirements for the Cultivation of Barbados Aloe (Aloe vera (L.) Burm. F.)—A Review

“…Pandhair et al (2011) showed that total sugars, fructose, sucrose, starch and phenol content were higher in micropropagated leaf gel as compared to sucker-propagated plants. Addition of 20 mg L −1 tryptophan induced a 2.43-fold increase in aloin content in multiple shoots cultures (Sharma V. et al, 2013). …”