Enhanced indirect organogenesis and efficient shoot multiplication of Petunia hybrida

Abstract

A STUDY was initiated through various experiments aiming to optimize different stages including callus induction, shoot regeneration, shoot multiplication, rooting and acclimatization of Petunia hybrida. Callogenesis from leaves of in vitro-grown seedling was observed on MS medium supplemented with 0.1 mg/l NAA combined with BAP at various concentrations (0.0, 0.25, 0.5 and 1.0 mg/l). The results indicated that the medium with BAP at 0.5 mg/l in combination with NAA at 0.1 mg/l resulted in the highest percentage for explants producing callus (40%). Callus was transferred to regeneration medium to study the effect of three types of cytokinins including BAP, TDZ and Kin at different concentrations (0.0, 0.2, 0.4, 0.6, 0.8 and 1.0 mg/l). The results showed that BAP at 0.2 mg/l or TDZ at 0.2 or 0.4 mg/l induced the highest multiplication capacity (90%). Another experiment employed four MS basal salt strengths (full, ¾, ½ and ¼ MS) in combination with three concentrations of sucrose (20, 30 and 40 g/l). Full strength MS basal salt medium with sucrose (40 g/l) boosted multiplication performance. Multiplied shoots were studied for rooting ability. Applying sucrose at the lowest concentration (15 g/l) with full or ½ MS produced the highest rooting percent (100%), the highest number of roots (8.33) per shoot and the longest roots (12.03 cm). The well rooted plantlets were successfully acclimatized on different potting media including peat moss, vermiculite, clay and perlite where clay surpassed other growing medium mixtures with a 89% survival rate.

Clonal Propagation of Rangoon Creeper (Quisqualis indica Linn.) Via Nodal Explant Culture

“…It could be concluded that the lowest concentrations of sucrose (15 g/l) and half MS was the best for root formation. These results confirm previous findings reported by Meziani, et al (2019) on date palm and Tawfik et al (2019) on petunia. This could be interpreted by the fact that media with small osmotic potential are favorite for root…”

“…The clay soil gave a survival rate of 20% and a shoot length of 3.8 cm. On contrary to this result, Tawfik et al, (2019) reported that clay soil recorded the highest survival rate (89 %) and the longest shoots (8.80 cm) among the all three media tested for petunia plantlets.…”

Multiplication and Rooting Shoot Tips and Nodes of Petunia Plant (Petunia hybrida)

“…resulted in the most shoots per explant (2.46 shoots), the longest (6.59 cm), and the most leaves per explant (28.20) after four weeks. [9] showed that growing the shoot tips and nodes of Datura innoxia on MS medium prepared with 0.5 mg.l -1 Kin gave the highest average number of shoots 14, 20 shoots/explant after 8 weeks, with average length of 1-3 cm, [10] reported that the highest response to tissue culture of Hyoscyamus muticus L. Nodes taken from seedlings resulted from histological cultured on MS medium prepared with 1.0 mg.l -1 Kin. with the highest average of shoots 8.20 shoots/explant and an average length of 1.37 cm after 4 weeks of planting.…”