Enhanced Keratinocyte Cell Attachment to Atelocollagen Thin Films Through Air and Nitrogen Plasma Treatment

García, Jorge López; Pachernı́k, Jiřı́; Lehocký, Marián; Junkar, Ita; Humpolíček, Petr; Sáha, Petr

doi:10.1007/978-3-642-19038-4_16

Cited by 8 publications

(8 citation statements)

References 18 publications

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“…As far as cell morphology is concerned, Figure 2 reveals the HaCaT cell growth on atelocollagen films with and without phenolic extracts, which shows cell aggregates in form of ripple-like areas adhered on the film surfaces. It may be also observed that some group of cells do not have the typical keratinocyte cell shapes, an anomaly that is probably a consequence of cell damage caused by the high concentration of phenols in the atelocollagen matrix [45,46].…”

Section: Resultsmentioning

confidence: 99%

Polyphenolic Extracts of Edible Flowers Incorporated onto Atelocollagen Matrices and Their Effect on Cell Viability

et al. 2013

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Abstract:The phenolic extract of chives flowers (Allium schoenoprasum, Liliaceae), introduced Sage (Salvia pratensis, Lamiaceae), European elderberry (Sambucus nigra, Caprifoliaceae) and common dandelion (Taraxacum officinale, Asteraceae) were characterised by High Performance Liquid Chromatography and incorporated in different concentrations onto atelocollagen thin films. In order to assess the biological impact of these phenolic compounds on cell viability, human immortalised non-tumorigenic keratinocyte cell line was seeded on the thin films and cell proliferation was determined by using an MTT assay. In addition, their antimicrobial activity was estimated by using an agar diffusion test. Data indicated the concomitance between cell viability and concentration of polyphenols. These findings suggest that these phenolic-endowed atelocollagen films might be suitable for tissue engineering applications, on account of the combined activity of polyphenols and collagen.

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Section: Resultsmentioning

confidence: 99%

Polyphenolic Extracts of Edible Flowers Incorporated onto Atelocollagen Matrices and Their Effect on Cell Viability

et al. 2013

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“…This phenomenon is a consequence of breaking bonds and free radicals formation that once the samples are withdrawn from the plasma reactor trigger the reaction between atmospheric oxygen and free radicals. The hydrophobic character of PTFE was altered making the specimens more hydrophilic after each treatment [ 21 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

Physical and Morphological Changes of Poly(tetrafluoroethylene) after Using Non-Thermal Plasma-Treatments

et al. 2018

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A commercial formulation of poly(tetrafluoroethylene) (PTFE) sheets were surface modified by using non-thermal air at 40 kHz frequency (DC) and 13.56 MHz radiofrequency (RF) at different durations and powers. In order to assess possible changes of PTFE surface properties, zeta potential (ζ), isoelectric points (IEPs) determinations, contact angle measurements as well as Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM) imaging were carried out throughout the experimentation. The overall outcome indicated that ζ-potential and surface energy progressively changed after each treatment, the IEP shifting to lower pH values and the implicit differences, which are produced after each distinct treatment, giving new surface topographies and chemistry. The present approach might serve as a feasible and promising method to alter the surface properties of poly(tetrafluoroethylene).

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“…It is foreseen through wound healing, transplantation and cell culture studies that HaCaT cells may be used as an in vitro model for highly proliferative epidermis in tissue engineering. The spontaneously immortalised HaCaT cell line has been a widely employed keratinocyte model due to its ease of propagation and near normal phenotype, but protocols for differentiation and gene delivery into HaCaT cells are extensively found in the literature [ 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

HaCaT Keratinocytes Response on Antimicrobial Atelocollagen Substrates: Extent of Cytotoxicity, Cell Viability and Proliferation

López-García

Lehocký

Humpolíček

et al. 2014

JFB

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The effective and widely tested biocides: Benzalkonium chloride, bronopol, chitosan, chlorhexidine and irgasan were added in different concentrations to atelocollagen matrices. In order to assess how these antibacterial agents influence keratinocytes cell growth, cell viability and proliferation were determined by using MTT assay. Acquired data indicated a low toxicity by employing any of these chemical substances. Furthermore, cell viability and proliferation were comparatively similar to the samples where there were no biocides. It means that regardless of the agent, collagen-cell-attachment properties are not drastically affected by the incorporation of those biocides into the substrate. Therefore, these findings suggest that these atelocollagen substrates enhanced by the addition of one or more of these agents may render effectiveness against bacterial stains and biofilm formation, being the samples referred to herein as “antimicrobial substrates” a promising view in the design of novel antimicrobial biomaterials potentially suitable for tissue engineering applications.

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Enhanced Keratinocyte Cell Attachment to Atelocollagen Thin Films Through Air and Nitrogen Plasma Treatment

Cited by 8 publications

References 18 publications

Polyphenolic Extracts of Edible Flowers Incorporated onto Atelocollagen Matrices and Their Effect on Cell Viability

Polyphenolic Extracts of Edible Flowers Incorporated onto Atelocollagen Matrices and Their Effect on Cell Viability

Physical and Morphological Changes of Poly(tetrafluoroethylene) after Using Non-Thermal Plasma-Treatments

HaCaT Keratinocytes Response on Antimicrobial Atelocollagen Substrates: Extent of Cytotoxicity, Cell Viability and Proliferation

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