Enhanced KRT13 gene expression bestows radiation resistance in squamous cell carcinoma cells

Nguyen, Tam Quang; Hamada, Atsuko; Yamada, Kaori; Higaki, Mirai; Shintani, Tomoaki; Yoshioka, Yukio; Toratani, Shigeaki; Okamoto, Tomoyoshi

doi:10.1007/s11626-020-00542-6

Cited by 4 publications

(4 citation statements)

References 36 publications

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“…Another study showed that KRT13 expression is increased in the tumor zone with a higher degree of stemness in prostate cancer [ 49 ]. Notably, a recent study showed that enhanced KRT13 expression is associated with radioresistance in squamous carcinoma cells, consistent with our current finding in pancreatic cancer [ 36 ]. We showed that si KRT13 treatment significantly enhanced the radiosensitivity of degron (++) cells after irradiation at 4 Gy, but not enhanced at 8 Gy.…”

Section: Discussionsupporting

confidence: 92%

“…Accordingly, we took notice of KRT13 among several candidates listed in Table 1 . During preparation of this manuscript, other investigators reported that KRT13 promotes the stemness of breast cancer cells and KRT13 is associated with radioresistance and stemness in squamous cell carcinoma cells, which further emphasized the importance of KRT13 in cancer stem cells [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

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KRT13is upregulated in pancreatic cancer stem-like cells and associated with radioresistance

Takenaka

Yokoyama

Ikehata

et al. 2023

Journal of Radiation Research

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Pancreatic cancer is one of the most aggressive cancers and the seventh leading cause of cancer-associated death in the world. Radiation is performed as an adjuvant therapy as well as anti-cancer drugs. Because cancer stem-like cells (CSCs) are considered to be radioresistant and cause recurrence and metastasis, understanding their properties is required for the development of novel therapeutic strategies. To investigate the CSC properties of pancreatic cancer cells, we used a pancreatic CSC model, degron (++) cells, which have low proteasome activity. Degron (++) cells displayed radioresistance in comparison with control cells. Using Ribonucleic acid (RNA) sequencing, we successfully identified KRT13 as a candidate gene responsible for radioresistance. Knockdown of KRT13 sensitized the degron (++) cells to radiation. Furthermore, a database search revealed that KRT13 is upregulated in pancreatic cancer cell lines and that high expression of KRT13 is associated with poorer prognosis. These results indicate that a combination therapy of KRT13 knockdown and radiation could hold therapeutic promise in pancreatic cancer.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

KRT13is upregulated in pancreatic cancer stem-like cells and associated with radioresistance

Takenaka

Yokoyama

Ikehata

et al. 2023

Journal of Radiation Research

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“…For up-regulated genes, KRT family and S100 family were reported that they were related to cell proliferation, cell apoptosis, metastasis, radiation resistance characteristics in many cancers. For instance, enhanced KRT13 gene expression bestows radiation resistance in squamous cell carcinoma cells ( Nguyen et al, 2021 ). KRT13 also promotes stemness and drives metastasis in breast cancer through a plakoglobin/c-Myc signaling pathway ( Yin et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

Role of TRPV1 ion channel in cervical squamous cell carcinoma genesis

Wang

Dong

Tian

et al. 2022

Front. Mol. Biosci.

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The transient receptor potential (TRP) family is a widely expressed superfamily of ion channels that regulate intracellular Ca2+ homeostasis and signal transduction. Abnormal expression of TRPV1 is closely related to malignant tumors of the female reproductive system such as breast, ovarian, cervical and endometrial cancers. In this study, we found a significant reduction of TRPV1 expression in cervical squamous cell carcinoma and this expression is inversely association with the risk of cervical squamous cell carcinoma. Furthermore, TRPV1 is involved in cell differentiation, iron death, inflammatory response, and metabolic regulation in cervical squamous cell carcinoma. Meanwhile TRPV1 is positively correlated with T cells and negatively associated with macrophages, indicating that TRPV is associated with tumor cell immunity. Therefore, TRPV1 may be a potential marker of cervical cancer and a promising anti-cancer drug candidate.

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“…Part of these genes have been reported in basic studies of pancreatic cancer and are closely related to the invasion and metastasis of pancreatic cancer according to the literature, including MYC ( Sodir et al, 2020 ), CDH2 ( Shintani et al, 2008 ), SNAI1 ( Dai et al, 2017 ), YAP1, SOX2 ( Zhang et al, 2017 ). Besides, these genes, ADAMTS2 ( Dey et al, 2020 ), KRT9 ( Andolino et al, 2018 ), KRT13 ( Nguyen et al, 2021 ), LY6D ( Barros-Silva et al, 2018 ), had been reported in a variety of cancers. GO enrichment analysis ( Table 1 ) was performed on the intersections.…”

Section: Resultsmentioning

confidence: 99%

Identification of DDX31 as a Potential Oncogene of Invasive Metastasis and Proliferation in PDAC

Xie

Liu

Ding

et al. 2022

Front. Cell Dev. Biol.

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Background: Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest malignant tumors worldwide and has poor prognosis. DEAD box proteins31 (DDX31) participate in cellular processes involving RNA secondary structure changes. However, the functions of DDX31 in PDAC remain to be elucidated.Methods: The key gene DDX31 was identified using a combination of a risk model and weighted gene co-expression network analysis (WGCNA) with R software. The biological functions of DDX31 in PDAC were investigated through bioinformatics analysis and in vitro experiments.Results: Combining with WGCNA and risk model, DDX31 was identified as a potential factor of the invasive metastasis properties of PDAC, and its expression was closely related to the malignant differentiation of PDAC. The results of gene set enrichment analysis (GSEA) showed that DDX31 was correlated with cell invasive metastasis and proliferation by activating MAPK signaling pathway. The inhibition of DDX31 inhibited the invasion and migration of PDAC cells. Survival analysis showed that DDX31 expression was negatively associated with the poor prognosis in patients with PDAC.Interpretation:DDX31 may be a potential factor for PDAC. The inhibition of DDX31 may be a potential way to treat PDAC.

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Enhanced KRT13 gene expression bestows radiation resistance in squamous cell carcinoma cells

Cited by 4 publications

References 36 publications

KRT13is upregulated in pancreatic cancer stem-like cells and associated with radioresistance

KRT13is upregulated in pancreatic cancer stem-like cells and associated with radioresistance

Role of TRPV1 ion channel in cervical squamous cell carcinoma genesis

Identification of DDX31 as a Potential Oncogene of Invasive Metastasis and Proliferation in PDAC

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