Enhanced microbial fuel cell (MFC) power outputs through Membrane Permeabilization using a branched polyethyleneimine

Soh, Sandrine M.; Lee, Dong Gyu; Mitchell, Robert J.

doi:10.1016/j.bios.2020.112623

Cited by 9 publications

(4 citation statements)

References 41 publications

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“…Figure S24 shows a strong correlation between the bioluminescence from the E. coli strain and its surviving population, implying the bioluminescence can be used as a proxy to evaluate viability. Subsequently, the efficacy of PEGtide[25.5.0] was evaluated against a number of E. coli strains that produce LPS of varying lengths (Figure d). , When compared against the wild-type cells, those strains producing the shortest LPS, i . e ., the Δ rfaC , Δ rfaD , Δ rfaE , Δ rfaF , and Δ rfaG mutants, were all more susceptible to PEGtide[25.5.0].…”

Section: Results and Discussionmentioning

confidence: 99%

“…Subsequently, the efficacy of PEGtide[25.5.0] was evaluated against a number of E. coli strains that produce LPS of varying lengths (Figure 2d). 38,39 When compared against the wild-type cells, those strains producing the shortest LPS, i.e., the ΔrfaC, ΔrfaD, ΔrfaE, ΔrfaF, and ΔrfaG mutants, were all more susceptible to PEGtide[25.5.0]. These results show that PEGtide[25.5.0] remained as effective against these mutants as the wild-type strain.…”

Section: Nmr and Gpc (Figures S8−s12)mentioning

confidence: 99%

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Antimicrobial PEGtides: A Modular Poly(ethylene glycol)-Based Peptidomimetic Approach to Combat Bacteria

et al. 2021

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Despite their high potency, the widespread implementation of natural antimicrobial peptides is still challenging due to their low scalability and high hemolytic activities. Herein, we address these issues by employing a modular approach to mimic the key amino acid residues present in antimicrobial peptides, such as lysine, leucine, and serine, but on the highly biocompatible poly(ethylene glycol) (PEG) backbone. A series of these PEG-based peptides (PEGtides) were developed using functional epoxide monomers, corresponding to each key amino acid, with several possessing highly potent bactericidal activities and controlled selectivities, with respect to their hemolytic behavior. The critical role of the composition and the structure of the PEGtides in their selectivities was further supported by coarse-grained molecular dynamic simulations. This modular approach is anticipated to provide the design principles necessary for the future development of antimicrobial polymers.

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Section: Results and Discussionmentioning

confidence: 99%

Section: Nmr and Gpc (Figures S8−s12)mentioning

confidence: 99%

Antimicrobial PEGtides: A Modular Poly(ethylene glycol)-Based Peptidomimetic Approach to Combat Bacteria

et al. 2021

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“…Auto-aggregation is associated with the development of biofilms [ 61 ], which would result if this also happened within our DCMFC. EPS promotes cell aggregation through complicated interactions and also accelerates the development of biofilms and granulation [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Statistical optimization of waste molasses-based exopolysaccharides and self-sustainable bioelectricity production for dual chamber microbial fuel cell by Bacillus piscis

Sakr,

Khater,

Kheiralla

et al. 2023

Microb Cell Fact

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Background The application of exopolysaccharide-producing bacteria (EPS) in dual chamber microbial fuel cells (DCMFC) is critical which can minimize the chemical oxygen demand (COD) of molasses with bioelectricity production. Hence, our study aimed to evaluate the EPS production by the novel strain Bacillus piscis by using molasses waste. Therefore, statistical modeling was used to optimize the EPS production. Its structure was characterized by UV, FTIR, NMR, and monosaccharides compositions. Eventually, to highlight B. piscis' adaptability in energy applications, bioelectricity production by this organism was studied in the BCMFC fed by an optimized molasses medium. Results B. piscis OK324045 characterized by 16S rRNA is a potent EPS-forming organism and yielded a 6.42-fold increase upon supplementation of molasses (5%), MgSO4 (0.05%), and inoculum size (4%). The novel exopolysaccharide produced by Bacillus sp. (EPS-BP5M) was confirmed by the structural analysis. The findings indicated that the MFC's maximum close circuit voltage (CCV) was 265 mV. The strain enhanced the performance of DCMFC achieving maximum power density (PD) of 31.98 mW m−2, COD removal rate of 90.91%, and color removal of 27.68%. Furthermore, cyclic voltammetry (CV) revealed that anodic biofilms may directly transfer electrons to anodes without the use of external redox mediators. Additionally, CV measurements made at various sweep scan rates to evaluate the kinetic studies showed that the electron charge transfer was irreversible. The SEM images showed the biofilm growth distributed over the electrode’s surface. Conclusions This study offers a novel B. piscis strain for EPS-BP5M production, COD removal, decolorization, and electricity generation of the optimized molasses medium in MFCs. The biosynthesis of EPS-BP5M by a Bacillus piscis strain and its electrochemical activity has never been documented before. The approach adopted will provide significant benefits to sugar industries by generating bioelectricity using molasses as fuel and providing a viable way to improve molasses wastewater treatment.

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“…Bioelectrochemical systems (BESs), a technology that takes advantage of electroactive microorganisms (EAMs) to transform chemical energy in wastewater into electrical energy, is widely valued for its simultaneous wastewater treatment and energy recovery (Jiang et al 2022, Soh et al 2020). As the structure, manipulation, microbiology, electrochemistry, and applications of BES have been intensively explored by previous generations for more than a decade, dozens of functions have been developed for BES (Wang &Ren 2013), involving microbial fuel cell (MFC), microbial electrolysis cell (MEC) (Shi et al 2022), and microbial electrosynthesis system (MES) (Srikanth et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Acclimation of electroactive biofilms under different operating conditions: comprehensive analysis from architecture, composition, and metabolic activity

Ma,

Dong,

Yan

et al. 2023

Environ Sci Pollut Res

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Electroactive bio lms (EABs) have aroused wide concern in waste treatment due to their unique capability of extracellular electron transfer with solid materials. The combined effect of different operating conditions on the formation, microbial architecture, composition, and metabolic activity of EABs are still unknown. In this study, the impact of three different factors (anode electrode, substrate concentration and resistance) on the acclimation and performance of EABs was investigated. The results showed that the shortest start-up time of 127.3 h and highest power density of 0.84 W m − 2 were obtained with carbon brush as electrode, low concentration of substrate (1.0 g L − 1 ) and 1000 Ω external resistance (denoted as N1). The EABs under N1 condition also represented strongest redox capacity, the lowest internal resistance, and close arrangement of bacteria. Moreover, the EABs cultured under different conditions both showed similar results, with direct electron transfer (DET) dominated from EABs to anode. Microbial community compositions indicated that EABs under N1 condition with lowest diversity and highest abundance of electroactive bacteria (46.68%). Higher substrate concentration (3.0 g L − 1 ) promoted the proliferation of some other bacteria without electroactivity, which was adverse to EABs. The metabolic analysis showed the difference of genes related to electron transfer (Cytochromes C and pili) and bio lm formation (xap) of EABs under different conditions, which further demonstrated the higher electroactivity of EABs under N1. These results provided a comprehensive understanding of the effect of different operating conditions on EABs including bio lm formation and electrochemical activity.

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Enhanced microbial fuel cell (MFC) power outputs through Membrane Permeabilization using a branched polyethyleneimine

Cited by 9 publications

References 41 publications

Antimicrobial PEGtides: A Modular Poly(ethylene glycol)-Based Peptidomimetic Approach to Combat Bacteria

Antimicrobial PEGtides: A Modular Poly(ethylene glycol)-Based Peptidomimetic Approach to Combat Bacteria

Statistical optimization of waste molasses-based exopolysaccharides and self-sustainable bioelectricity production for dual chamber microbial fuel cell by Bacillus piscis

Acclimation of electroactive biofilms under different operating conditions: comprehensive analysis from architecture, composition, and metabolic activity

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