Enhanced neuronal regeneration by the combination of cannabidiol (CBD) with CB1 and CB2 antagonists following peripheral nerve axotomy.

Abstract

We have previously shown that cannabidiol (CBD) is neuroprotective following peripheral axotomy. Nevertheless, the role of endogenous cannabinoid receptors has not been investigated, what may in turn enhance the therapeutic use of substances derived from Cannabis. We show herein that CBD treatment following axotomy downregulates vesicular glutamate transporter 1 (VGLUT-1) and growth associated protein (GAP-43) expression. Such effect is reversed by the application of CB1 and CB2 antagonists, indicating that endogenous cannabinoid receptors are involved in the regenerative response to injury. Interestingly, motor function recovery was improved with the selective combination of CBD and receptor antagonists. The findings of the present work may be used to improve the effects of cannabinoid treatment following nervous system lesion, what may in turn enhance translational medicine application.