Enhanced neuroprotective effect by combination of bromocriptine and Hypericum perforatum extract against MPTP-induced neurotoxicity in mice

Mohanasundari, M; Srinivasan, M.; Sethupathy, S; Sabesan, M

doi:10.1016/j.jns.2006.06.018

Cited by 65 publications

(33 citation statements)

References 27 publications

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“…The mice of both groups soon recovered, as seen in other studies [20]. The ability to balance and walk on a rotating rod is widely used as a measure of motor skill and coordination [13]. MPTP-injected mice displayed a reduced mean hind limb stride length compared with their forelimbs, resembling the reduced stride length in PD gait [14].…”

Section: Discussionsupporting

confidence: 68%

“…The fourth group was treated with Ws orally for 28 days. At the end of the experiment (28 days after the first MPTP injection), the animals were analysed in behavioral studies such as the Rotarod test [8], Hang test [13] and stride length measurement [14]. The mice were killed by cervical dislocation on the following day, and their brains were dissected via the method described by Glowinski and Iversen [15] to procure the midbrains for the analyses of TBARS [16], SOD [17], CAT [18] and protein [19].…”

Section: Experimental Designmentioning

confidence: 99%

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The neuroprotective effect of Withania somnifera root extract in MPTP-intoxicated mice: An analysis of behavioral and biochemical varibles

Sankar

Manivasagam

Krishnamurti

et al. 2007

Cellular and Molecular Biology Letters

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Abstract:We studied the influence of Withania somnifera (Ws) root extract (100 mg/kg body weight) on parkinsonism induced by 1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine (MPTP; i.p, 20 mg/kg body weight for 4 days), via the analysis of behavioral features and the oxidant-antioxidant imbalance in the midbrain of mice. A significant alteration in behavior, increased levels of thiobarbituric acid reactive substance (TBARS), and increased activities of superoxide dismutase (SOD) and catalase (CAT) were noticed in this region of brain in MPTP-treated mice. Oral treatment with the root extract resulted in a significant improvement in the mice's behavoiur and antioxidant status, along with a significant reduction in the level of lipid peroxidation. The results indicated that at least part of the chronic stress-induced pathology may be due to oxidative stress, which is mitigated by Ws. Further studies are needed to assess the precise mechanism to support the clinical use of the plant as an antiparkinsonic drug.

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Section: Discussionsupporting

confidence: 68%

Section: Experimental Designmentioning

confidence: 99%

The neuroprotective effect of Withania somnifera root extract in MPTP-intoxicated mice: An analysis of behavioral and biochemical varibles

Sankar

Manivasagam

Krishnamurti

et al. 2007

Cellular and Molecular Biology Letters

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“…Previous studies have also showed necrosis of cells, cellular oedema, local microglial activation, rosenthal fibres and extensive gliosis in MPTP treated mice. 33,17 Local microglial activation within the nigrostriatum is a secondary reaction to dopaminergic neuro degeneration. Microglial activation can also exacerbate ROS production.…”

Section: Histopathological Evaluationmentioning

confidence: 99%

Effect of silybin in 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine (MPTP) induced parkinsonism in mice

Justin¹,

Gobalakrishnan

Asirvatham³

2017

Int J Basic Clin Pharmacol

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Background: Parkinson disease (PD) is a major neurological disorder known since ancient times. Though drugs are available for therapy, still effective drug targeting the etiopathogenesis is always going on.Methods: After obtaining permission from animal ethics committee, the mice were divided into four groups of eight each (normal control, experimental control with normal diet only, silybin 300mg/kg, silybin 600mg/kg). At the end of 55 days the mice were subjected to overnight fasting followed by plasma and liver biochemical analysis.Results: The mice treated with 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine (MPTP) developed motor dysfunction and behavioural changes similar to PD, which was tested with rotarod test, photoactometer test and hang test which revealed the impaired performance, hypo-locomotion and impaired neuromuscular strength respectively. Treatment with silybin reversed the motor dysfunction significantly (p≤0.001) in a dose dependent manner. Biochemical analysis measured the oxidant (TBARS, SOD, CAT) and antioxidants (GPx and GSH) which revealed the oxidant activity of MPTP and antioxidant activity of silybin. Histopathological evaluation showed the cytoprotective effect of silybin.Conclusions: Silybin by its antioxidant property has a neuroprotective activity both in motor activity and behaviourally in the MPTP induced Parkinson disease in mice. Hence present study offers a conclusive evidence that silybin is a neuroprotective. Diet supplemented with silybin can protect against neurodegenerative disorders and prevent the progression of neurodegeneration.

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“…The hanging test was performed as previously described by [20] . Briefly, mice were placed on a horizontal grid and inverted upside down.…”

Section: Hanging Testmentioning

confidence: 99%

In Invivo model of Parkinson’s syndrome, concurrent administrations of anti-inflammatory drugs changed behavioral deficits

Oluwole

Olajire²

2015

Inflamm Cell Signal

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In most of the laboratory and occupational studies, Parkinson's syndrome (PS) is the major outcome of paraquat (PQ) toxicities. Nevertheless, the search for novel therapy against PS is still enormous. We therefore examined the effects of anti-inflammatory drugs against PS. Swiss mice (30-35g) were pretreated orally with saline, distilled water, acetylsalicylate acid (10mg/kg) or prednisolone (5mg/kg) for 7days. They were concurrently treated for another 5 weeks against intraperitoneal injection of 0.01ml and 0.02ml of (10mg/kg) PQ which were given twice a week to induced PS. Subsequently, movement deficits in mice were closely examined by using the spontaneous motor activity test, the catalepsy, and hanging tests. Thereafter, the mice brains were dissected for the biochemical tests. The data were analyzed using ANOVA, and the values were statistically significant at (p<0.0001, p<0.05). In conclusion, aspirin and prednisolone produced strong antioxidant effects that may be associated with change in the behavioral deficits.

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Enhanced neuroprotective effect by combination of bromocriptine and Hypericum perforatum extract against MPTP-induced neurotoxicity in mice

Cited by 65 publications

References 27 publications

The neuroprotective effect of Withania somnifera root extract in MPTP-intoxicated mice: An analysis of behavioral and biochemical varibles

The neuroprotective effect of Withania somnifera root extract in MPTP-intoxicated mice: An analysis of behavioral and biochemical varibles

Effect of silybin in 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine (MPTP) induced parkinsonism in mice

In Invivo model of Parkinson’s syndrome, concurrent administrations of anti-inflammatory drugs changed behavioral deficits

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