Enhanced PC12 Cells Proliferation with Self-Assembled S-Layer Proteins Scaffolds

Babolmorad, Ghazal; Emtiazi, Giti; Ghaedi, Kamran; Jodeiri, Mohamad

doi:10.1007/s12010-014-1248-9

Cited by 7 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Babolmorad et al (2015) reported that the viability of PC12 cells on the PLO 9% S-layer scaffold showed higher growth than the control (PLO-laminin) only after 24 hrs of cell seeding. 29 They could not do their experiments for more than 24 hrs. It may be due to the S-layer was not sterilized by formalin gas, so the scaffold might be contaminated.…”

Section: Cell-scaffolds Interaction and Metabolic Activitiesmentioning

confidence: 99%

<p>The Efficacy of Electrospun PAN/Kefiran Nanofiber and Kefir in Mammalian Cell Culture: Promotion of PC12 Cell Growth, Anti-MCF7 Breast Cancer Cells Activities, and Cytokine Production of PBMC</p>

Jenab

Roghanian

Ghorbani

et al. 2020

IJN

Self Cite

View full text Add to dashboard Cite

Background: Kefiran is a useful polysaccharide made of branched glucogalactose which is produced by microorganisms. Here the anti-MCF-7 breast cancer cells activity of kefiran and cytokine productions (IL-6) of peripheral blood mononuclear cells (PBMC) treated by kefiran was studied. Also, the effect of using kefiran as a useful and cost-effective scaffold in neural stem cell culture (PC12 cell culture) was investigated. Material and Methods: Kefiran was produced from raw milk with 0.5% fat and 10 g of kefir grains. After incubation for 48 hrs at room temperature, the solvent collected (crude kefiran). These samples were kept at 100°C for 1 hr (boiled kefiran) and the supernatant was precipitated by ethanol (pure kefiran). Then, the electrospun nanofibers, pure polyacrylonitrile (PAN), PAN/kefiran 5%, and PAN/kefiran 10% were fabricated and used as scaffolds in the cell culture. The structure of fabricated was studied by SEM and the cytokine production (IL-6) in vitro in the cell culture supernatant of PBMC line after treatment with kefiran (1mg/mL, 5 mg/mL) and kefiran-PAN 5% and 10% were carried out by enzyme-linked immunosorbent assay (ELISA). The attachment of PC12 cells was examined by inverted microscope. Also, cytotoxicity of kefiran for PC12 and MCF7 cells and morphological changes of PC12 cells were evaluated by MTT and Cresyl violet staining (Nissl staining) respectively. Results: The mean diameter of fabricated PAN/kefiran 5% and 10% nanofibers were 310.2±43.97 nm. The contact angle measurement results (26.9± 1.9 for the pure PAN scaffold vs 12.3± 1.13 for the PAN/kefiran) revealed enhanced hydrophilicity of scaffolds upon the incorporation of kefiran and PAN. Seeding of PC12 cells on the scaffolds showed that fabrication of kefiran into PAN led to the enhancement of cell attachment, proliferation, and morphological changes. Also, the promotion of PBMC growth and decreasing of MCF7 cell lines viability were shown through MTT assay. No significant changes were measured for the level of IL-6 in PAN/kefiran 5% treated cells compared to the control (p ≥ 0.05). Conclusion: These results suggest superior properties of kefiran/PAN nanofibrous scaffolds for the neural stem cell culture especially for repairing injured spinal cord. Also, the pure kefiran could be used for the enhancement of PBMC growth and reducing the MCF7 cancerous cells growth. So, using biocompatible, anti-bacterial, and anti-tumor kefiran/ PAN nanofibers for regenerative medicine seems promising.

show abstract

Section: Cell-scaffolds Interaction and Metabolic Activitiesmentioning

confidence: 99%

<p>The Efficacy of Electrospun PAN/Kefiran Nanofiber and Kefir in Mammalian Cell Culture: Promotion of PC12 Cell Growth, Anti-MCF7 Breast Cancer Cells Activities, and Cytokine Production of PBMC</p>

Jenab

Roghanian

Ghorbani

et al. 2020

IJN

Self Cite

View full text Add to dashboard Cite

show abstract

“…Finally DF determines a general function that should be maximum following selection of optimum value of affective variables with consideration of their interaction . The main advantages of DFs are the ability to obtain qualitative and quantitative responses by simple and fast transformation of various responses for one measurement …”

Section: Methodsmentioning

confidence: 99%

“…[40,41] The main advantages of DFs are the ability to obtain qualitative and quantitative responses by simple and fast transformation of various responses for one measurement. [42]…”

Section: Experimental Designmentioning

confidence: 99%

Synthesis of carbon nanotube‐based nanocomposite and application for wastewater treatment by ultrasonicated adsorption process

Azqhandi

Shekari

Ghalami‐Choobar

2018

Applied Organom Chemis

View full text Add to dashboard Cite

The sorption of methylene blue (MB) and basic yellow 28 (BY28) dyes in water on Ag@ZnO/MWCNT (Ag‐doped ZnO loaded on multiwall carbon nanotubes) nanocomposite is investigated in a batch process, optimizing starting initial dye concentration, sonication time and adsorbent mass. Isotherms and kinetic behaviours of MB and BY28 adsorption onto Ag@ZnO/MWCNT were explained by extended Freundlich and pseudo‐second‐order kinetic models. Ag@ZnO/MWCNT was synthesized and characterized using X‐ray diffraction, energy‐dispersive X‐ray spectroscopy, field emission scanning electron microscopy and Brunauer–Emmett–Teller analysis. According to the experimental data, adaptive neuro‐fuzzy inference system (ANFIS), generalized regression neural network (GRNN), backpropagation neural network (BPNN), radial basic function neural network (RBFNN) and response surface methodology (RSM) were developed, and applied to forecast the removal performance of the sorbent. The influence of process variables (i.e. sonication time, initial dye concentration, adsorbent mass) on the removal of MB and BY28 was considered by central composite rotatable design of RSM, GRNN, ANFIS, BPNN and RBFNN. The performances of the developed ANFIS, GRNN, BPNN and RBFNN models were compared with RSM mathematical models in terms of the root mean square error, coefficient of determination, absolute average deviation and mean absolute error. The coefficients of determination calculated from the validation data for ANFIS, GRNN, BPNN, RBFNN and RSM models were 0.9999, 0.9997, 0.9883, 0.9898 and 0.9608 for MB and 0.9997, 0.9990, 0.9859, 0.9895 and 0.9593 for BY28 dye, respectively. The ANFIS model was found to be more precise compared to the other models. However, the GRNN method is much easier than the ANFIS method and needs less time for analysis. So, it has potential in chemometrics and it is feasible that the GRNN algorithm could be applied to model real systems. The monolayer adsorption capacity of MB and BY28 was 292.20 and 287.02 mg g−1, respectively.

show abstract

“…A broad majority of these studies obtained an advantage of the topography imaging aspects of the technique. In them, the reported research showed the analysis of a large range of materials such as natural and synthetic polymers [52,53,54], collagen [55], silk fibroin [56,57,58], cellulose [59], peptides [60,61], graphene and carbon nanotubes (CNT) [62,63], particles [64,65], or patterned structures [66,67]. This list, kept short for compact design reasons, could include more field-related articles.…”

Section: Afm and Cell Scaffolds: Literature Reviewmentioning

confidence: 99%

Characterization of Cell Scaffolds by Atomic Force Microscopy

Iturri

Toca-Herrera

2017

Polymers

View full text Add to dashboard Cite

This review reports on the use of the atomic force microscopy (AFM) in the investigation of cell scaffolds in recent years. It is shown how the technique is able to deliver information about the scaffold surface properties (e.g., topography), as well as about its mechanical behavior (Young's modulus, viscosity, and adhesion). In addition, this short review also points out the utilization of the atomic force microscope technique beyond its usual employment in order to investigate another type of basic questions related to materials physics, chemistry, and biology. The final section discusses in detail the novel uses that those alternative measuring modes can bring to this field in the future.

show abstract

Enhanced PC12 Cells Proliferation with Self-Assembled S-Layer Proteins Scaffolds

Cited by 7 publications

References 15 publications

<p>The Efficacy of Electrospun PAN/Kefiran Nanofiber and Kefir in Mammalian Cell Culture: Promotion of PC12 Cell Growth, Anti-MCF7 Breast Cancer Cells Activities, and Cytokine Production of PBMC</p>

<p>The Efficacy of Electrospun PAN/Kefiran Nanofiber and Kefir in Mammalian Cell Culture: Promotion of PC12 Cell Growth, Anti-MCF7 Breast Cancer Cells Activities, and Cytokine Production of PBMC</p>

Synthesis of carbon nanotube‐based nanocomposite and application for wastewater treatment by ultrasonicated adsorption process

Characterization of Cell Scaffolds by Atomic Force Microscopy

Contact Info

Product

Resources

About