Enhanced peak responses due to solvent interactions in high-performance liquid chromatography

Sample Preparation and High-Performance Liquid Chromatographic Analysis of Deoxyribonucleoside Triphosphates in Individual Rat Embryos

“…1, Sample). An explanation for these anomalies may lie in the composition of the sample solvent, which can have a significant impact on the chromatographic behavior of sample components, particularly those eluting early (12)(13)(14). The aberrant behavior observed is most likely due to a transient disturbance of chromatographic conditions introduced by injection of sample bolus into the fluid stream.…”

Theoretical study of system peaks in linear chromatography

“…Vacancy chromatography, which is a special case of system peaks where the sample contains only the weak solvent, was described much earlier, in gas chromatography (2). The occurrence of system peaks has been explained by 0003-2700/90/0362-0923$02.50/0 © 1990 American Chemical Society solvent displacement, solvophobic interactions in the mobile phase, preferential evaporation of one component of the mixed mobile phase during storage (3), preferential solvation (4), intramolecular hydrogen bonding, or hydrophobic interactions (5). The origin and properties of system peaks have been reviewed recently by Levin and Grushka (6,7).…”

“…The purpose of the present paper is an in-depth, quantitative discussion of the system peaks for nonionic systems, under experimental conditions such that the phenomenon appears to be linear. Although a number of important papers have been published in this area (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14), there is still no theoretical model permitting a quantitative prediction of the system peaks.…”

Simultaneous determination of triamcinolone acetonide and oxymetazoline hydrochloride in nasal spray formulations by HPLC

“…TAA has been determined by many analytical methods, such as visible and derivative spectrophotometry [4,5], highperformance liquid chromatography (HPLC) [6][7][8] and gas chromatography [9]. OXY has been determined by high-performance liquid chromatography [10][11][12].…”