Enhanced Photodynamic Therapy by Improved Light Energy Capture Efficiency of Porphyrin Photosensitizers

Tian, Zejie; Li, Hui; Liu, Zhenhua; Yang, Lichao; Zhang, Chaoyang; He, Jun; Ai, Wenbin; Liu, Yunmei

doi:10.1007/s11864-023-01120-0

Cited by 12 publications

(6 citation statements)

References 105 publications

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“…Therefore, it may be possible to develop fluorescent probes that can specifically capture survivin in vivo by fusing them with the SSFPs developed in this study. Additionally, SSFPs may be applied to survivin-targeting activatable photodynamic cancer therapy using appropriate FRET-activatable probes with a photosensitizer . This study is a pilot investigation into the development of linear peptides that may encounter challenges related to metabolic stability.…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, SSFPs may be applied to survivin-targeting activatable photodynamic cancer therapy using appropriate FRET-activatable probes with a photosensitizer. 28 This study is a pilot investigation into the development of linear peptides that may encounter challenges related to metabolic stability. Therefore, toxicity and stability assessments of these peptides were not conducted, as they are intended for use only in in vitro experiments.…”

Section: Evaluation Of Ssfps As Fret Probesmentioning

confidence: 99%

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Peptide-Based Turn-On Fluorescent Probes for Highly Specific Detection of Survivin Protein in the Cancer Cells

Fuchigami,

Nakayama,

Miyanari

et al. 2024

Chemical & Biomedical Imaging

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Survivin is highly expressed in most human cancers, making it a promising target for cancer diagnosis and treatment. In this study, we developed peptide probes consisting of Bor 65−75 , a highaffinity survivin-binding peptide, and a survivin protein segment using peptide linkers as survivin-sensitive fluorescent probes (SSFPs). All conjugates were attached to 5(6)-carboxyfluorescein (FAM) at the Cterminal as a fluorophore and to 4((4(dimethylamino)phenyl)azo)benzoic acid (DABCYL) at the N-terminal as a quencher. Fluorescence (or Forster) resonance energy transfer (FRET) quenching via intramolecular binding of Bor 65−75 with survivin protein segment could be diminished by the approach of survivin to SSFPs, which dissociate Bor 65−75 from SSPF and increased the distance between FAM and DABCYL. A binding assay using recombinant human survivin protein (rSurvivin) demonstrated moderate to high affinity of SSFPs for survivin (dissociation constants (K d ) = 121−1740 nM). Although the SSFPs (0.5 μM) had almost no fluorescence under baseline conditions, a dose-dependent increase in fluorescence intensity was observed in the presence of rSurvivin (0.1−2.0 μM). In particular, the proline-rich SSFP (SSFP5) showed the highest (2.7-fold) fluorescence induction at 2.0 μM survivin compared to the signals in the absence of survivin. Confocal fluorescence imaging demonstrated that SSFP5 exhibited clear fluorescence signals in survivin-positive MDA-MB-231 cells, whereas no marked fluorescence signals were observed in survivin-negative MCF-10A cells. Collectively, these results suggest that SSFPs can be used as survivin-specific FRET imaging probes.

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Section: Resultsmentioning

confidence: 99%

Section: Evaluation Of Ssfps As Fret Probesmentioning

confidence: 99%

Peptide-Based Turn-On Fluorescent Probes for Highly Specific Detection of Survivin Protein in the Cancer Cells

Fuchigami,

Nakayama,

Miyanari

et al. 2024

Chemical & Biomedical Imaging

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“…Porphyrin’s name comes from porphyra, a Greek word that means purple, because porphyrins are usually red or bright purple. Porphyrins are widely used in PDT owing to the unique photosensitive porphyrins [ 62 ]. In fact, several studies were carried out with porphyrins as photosensitizers in PDT.…”

Section: Nanotechnology In Photodynamic Therapymentioning

confidence: 99%

Recent Innovations of Mesoporous Silica Nanoparticles Combined with Photodynamic Therapy for Improving Cancer Treatment

Nady,

Hassan,

Amin

et al. 2023

Pharmaceutics

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Cancer is a global health burden and is one of the leading causes of death. Photodynamic therapy (PDT) is considered an alternative approach to conventional cancer treatment. PDT utilizes a light-sensitive compound, photosensitizers (PSs), light irradiation, and molecular oxygen (O2). This generates cytotoxic reactive oxygen species (ROS), which can trigger necrosis and/ or apoptosis, leading to cancer cell death in the intended tissues. Classical photosensitizers impose limitations that hinder their clinical applications, such as long-term skin photosensitivity, hydrophobic nature, nonspecific targeting, and toxic cumulative effects. Thus, nanotechnology emerged as an unorthodox solution for improving the hydrophilicity and targeting efficiency of PSs. Among nanocarriers, mesoporous silica nanoparticles (MSNs) have gained increasing attention due to their high surface area, defined pore size and structure, ease of surface modification, stable aqueous dispersions, good biocompatibility, and optical transparency, which are vital for PDT. The advancement of integrated MSNs/PDT has led to an inspiring multimodal nanosystem for effectively treating malignancies. This review gives an overview of the main components and mechanisms of the PDT process, the effect of PDT on tumor cells, and the most recent studies that reported the benefits of incorporating PSs into silica nanoparticles and integration with PDT against different cancer cells.

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“…On the other hand, the lights for the excitation of such complexes fall mostly in the blue range (400–430 nm) (Table 1), 10,13–15 and the low-efficiency of such lights for deep-tissue excitation of the probe significantly limits the practical applications of such probes. The efforts to enlarge the spectral window for probe-excitation, 16–19 have only been met with limited success with a sole encouraging case of Yb( iii ) emission being co-sensitized by porphyrin and arguably the most extensively used fluorescence dye of boron-dipyrromethene (BODIPY) in the 450-to-560 nm range. 18 Other dyes with strong visible light absorption have been explored for a similar capacity.…”

Section: Introductionmentioning

confidence: 99%

Lanthanide complexes with an azo-dye chromophore ligand: syntheses, crystal structures, and near-infrared luminescence by long-wavelength excitation

Chen,

Feng,

Zhu

et al. 2024

Dalton Trans.

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Enhanced Photodynamic Therapy by Improved Light Energy Capture Efficiency of Porphyrin Photosensitizers

Cited by 12 publications

References 105 publications

Peptide-Based Turn-On Fluorescent Probes for Highly Specific Detection of Survivin Protein in the Cancer Cells

Peptide-Based Turn-On Fluorescent Probes for Highly Specific Detection of Survivin Protein in the Cancer Cells

Recent Innovations of Mesoporous Silica Nanoparticles Combined with Photodynamic Therapy for Improving Cancer Treatment

Lanthanide complexes with an azo-dye chromophore ligand: syntheses, crystal structures, and near-infrared luminescence by long-wavelength excitation

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