Enhanced stability of freestanding lipid bilayer and its stability criteria

Jeong, Dae-Woong; Jang, Hyunwoo; Choi, Siyoung Q.; Choi, Myung Chul

doi:10.1038/srep38158

Cited by 20 publications

(17 citation statements)

References 42 publications

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“…This tendency of the contact angle agreed with a previous report using Span 80 and lipids by Jeong et al, in which a droplet adhered to a flat interface covered with a surfactant monolayer and formed DIB between it and the contact interface [ 32 ]. They illustrated that Span 80 was removed from the contact interface during the DIB formation.…”

Section: Resultssupporting

confidence: 92%

Microfluidic Formation of Honeycomb-Patterned Droplets Bounded by Interface Bilayers via Bimodal Molecular Adsorption

et al. 2020

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Assembled water-in-oil droplets bounded by lipid bilayers are used in synthetic biology as minimal models of cell tissue. Microfluidic devices successfully generate monodispersed droplets and assemble them via droplet interface bilayesr (DIB) formation. However, a honeycomb pattern of DIB-bounded droplets, similar to epithelial tissues, remains unrealized because the rapid DIB formation between the droplets hinders their ability to form the honeycomb pattern. In this paper, we demonstrate the microfluidic formation of a honeycomb pattern of DIB-bounded droplets using two surfactants with different adsorption rates on the droplet surface. A non-DIB forming surfactant (sorbitan monooleate, Span 80) was mixed with a lipid (1,2-dioleoyl-sn-glycero-3-phosphocholine, PC), whose adsorption rate on the droplet surface and saturated interfacial tension were lower than those of Span 80. By changing the surfactant composition, we established the conditions under which the droplets initially form a honeycomb pattern and subsequently adhere to each other via DIB formation to minimize the interfacial energy. In addition, the reconstituted membrane protein nanopores at the DIBs were able to transport molecules. This new method, using the difference in the adsorption rates of two surfactants, allows the formation of a honeycomb pattern of DIB-bounded droplets in a single step, and thus facilitates research using DIB-bounded droplet assemblies.

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Section: Resultssupporting

confidence: 92%

Microfluidic Formation of Honeycomb-Patterned Droplets Bounded by Interface Bilayers via Bimodal Molecular Adsorption

et al. 2020

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“…Lipid vesicles 43 – 45 , supported lipid bilayers (SLB) 46 – 49 , and freestanding lipid bilayers 50 can provide a valuable in vitro-platform for the investigation of a variety of biological questions associated with cell membrane function, structure, and composition 51 , 52 . Of those, SLB as a lipid bilayer film on solid supports can mimic the key architectural features of cellular membranes.…”

Section: Introductionmentioning

confidence: 99%

A combination of electrochemistry and mass spectrometry to monitor the interaction of reactive species with supported lipid bilayers

Ravandeh

Kahlert

Jablonowski

et al. 2020

Sci Rep

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Reactive oxygen and nitrogen species (RONS), e.g. generated by cold physical plasma (CPP) or photodynamic therapy, interfere with redox signaling pathways of mammalian cells, inducing downstream consequences spanning from migratory impairment to apoptotic cell death. However, the more austere impact of RONS on cancer cells remains yet to be clarified. In the present study, a combination of electrochemistry and high-resolution mass spectrometry was developed to investigate the resilience of solid-supported lipid bilayers towards plasma-derived reactive species in dependence of their composition. A 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipid bilayer was undisturbed by 200 µM H2O2 (control) but showed full permeability after CPP treatment and space-occupying oxidation products such as PoxnoPC, PAzePC, and POPC hydroperoxide were found. Electron paramagnetic resonance spectroscopy demonstrated the presence of hydroxyl radicals and superoxide anion/hydroperoxyl radicals during the treatment. In contrast, small amounts of the intramembrane antioxidant coenzyme Q10 protected the bilayer to 50% and LysoPC was the only POPC derivative found, confirming the membrane protective effect of Q10. Such, the lipid membrane composition including the presence of antioxidants determines the impact of pro-oxidant signals. Given the differences in membrane composition of cancer and healthy cells, this supports the application of cold physical plasma for cancer treatment. In addition, the developed model using the combination of electrochemistry and mass spectrometry could be a promising method to study the effect of reactive species or mixes thereof generated by chemical or physical sources.

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“…To improve DIB formation with DOPC, other groups have recently shown that amphiphilic compounds (such as SDS and SPAN) used as co-surfactants with lipids promote sufficient monolayer formation and enable the assembly of stable DOPC DIBs even with large droplets. [20][21][22] These findings were explained in the context of lipid-shape factor; addition of cosurfactants with a conical shape that compliments the inverted conical shape of DOPC enables tighter monolayer packing and a better coverage of the oil-water interface. However, the presence of surface active polymers in a lipid-based monolayer or bilayer is known to alter the transport properties of the membrane and, more importantly, may not accurately mimic the membranes of living cells.…”

Section: Introductionmentioning

confidence: 99%

Evaporation-induced monolayer compression improves droplet interface bilayer formation using unsaturated lipids

et al. 2018

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In this article, we report on a new experimental methodology to enable reliable formation of droplet interface bilayer (DIB) model membranes with two types of unsaturated lipids that have proven difficult for creating stable DIBs. Through the implementation of a simple evaporation technique to condition the spontaneously assembled lipid monolayer around each droplet, we increased the success rates of DIB formation for two distinct unsaturated lipids, namely 1,2-dioleoyl--glycero-3-phosphocholine (DOPC) and 1-palmitoyl-2-oleoyl--glycero-3-phosphocholine (POPC), from less than 10% to near 100%. Separately, using a pendant drop tensiometer, we learned that: (a) DOPC and POPC monolayers do not spontaneously assemble into their tightest possible configurations at an oil-water interface, and (b) reducing the surface area of a water droplet coated with a partially packed monolayer leads to a more tightly packed monolayer with an interfacial tension lower than that achieved by spontaneous assembly alone. We also estimated from Langmuir compression isotherms obtained for both lipids that the brief droplet evaporation procedure prior to DIB formation resulted in a 6%-16% reduction in area per lipid for DOPC and POPC, respectively. Finally, the increased success rates of formation for DOPC and POPC DIBs enabled quantitative characterization of unsaturated lipid membrane properties including electrical resistance, rupture potential, and specific capacitance.

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Enhanced stability of freestanding lipid bilayer and its stability criteria

Cited by 20 publications

References 42 publications

Microfluidic Formation of Honeycomb-Patterned Droplets Bounded by Interface Bilayers via Bimodal Molecular Adsorption

Microfluidic Formation of Honeycomb-Patterned Droplets Bounded by Interface Bilayers via Bimodal Molecular Adsorption

A combination of electrochemistry and mass spectrometry to monitor the interaction of reactive species with supported lipid bilayers

Evaporation-induced monolayer compression improves droplet interface bilayer formation using unsaturated lipids

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